LEWIS FLETCHER BUSS
Projetos de Pesquisa
Unidades Organizacionais
LIM/46 - Laboratório de Parasitologia Médica, Hospital das Clínicas, Faculdade de Medicina
4 resultados
Resultados de Busca
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- Epidemiology of COVID-19 after Emergence of SARS-CoV-2 Gamma Variant, Brazilian Amazon, 2020-2021(2022) NICOLETE, Vanessa C.; RODRIGUES, Priscila T.; FERNANDES, Anderson R. J.; CORDER, Rodrigo M.; TONINI, Juliana; BUSS, Lewis F.; SALES, Flavia C.; FARIA, Nuno R.; SABINO, Ester C.; CASTRO, Marcia C.; FERREIRA, Marcelo U.The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) Gamma variant has been hypothesized to cause more severe illness than previous variants, especially in children. Successive SARS-CoV-2 IgG serosurveys in the Brazilian Amazon showed that agespecific attack rates and proportions of symptomatic SARS-CoV-2 infections were similar before and after Gamma variant emergence.
- Epidemiological and clinical characteristics of the COVID-19 epidemic in Brazil(2020) SOUZA, William Marciel de; BUSS, Lewis Fletcher; CANDIDO, Darlan da Silva; CARRERA, Jean-Paul; LI, Sabrina; ZAREBSKI, Alexander E.; PEREIRA, Rafael Henrique Moraes; PRETE JR., Carlos A.; SOUZA-SANTOS, Andreza Aruska de; PARAG, Kris V.; BELOTTI, Maria Carolina T. D.; VINCENTI-GONZALEZ, Maria F.; MESSINA, Janey; SALES, Flavia Cristina da Silva; ANDRADE, Pamela dos Santos; NASCIMENTO, Vitor Heloiz; GHILARDI, Fabio; ABADE, Leandro; GUTIERREZ, Bernardo; KRAEMER, Moritz U. G.; BRAGA, Carlos K. V.; AGUIAR, Renato Santana; ALEXANDER, Neal; MAYAUD, Philippe; BRADY, Oliver J.; MARCILIO, Izabel; GOUVEIA, Nelson; LI, Guangdi; TAMI, Adriana; OLIVEIRA, Silvano Barbosa de; PORTO, Victor Bertollo Gomes; GANEM, Fabiana; ALMEIDA, Walquiria Aparecida Ferreira de; FANTINATO, Francieli Fontana Sutile Tardetti; MACARIO, Eduardo Marques; OLIVEIRA, Wanderson Kleber de; NOGUEIRA, Mauricio L.; PYBUS, Oliver G.; WU, Chieh-Hsi; CRODA, Julio; SABINO, Ester C.; FARIA, Nuno RodriguesBrazil has one of the fastest-growing COVID-19 epidemics in the world. De Souza et al. report epidemiological, demographic and clinical findings for COVID-19 cases in the country during the first 3 months of the epidemic. The first case of COVID-19 was detected in Brazil on 25 February 2020. We report and contextualize epidemiological, demographic and clinical findings for COVID-19 cases during the first 3 months of the epidemic. By 31 May 2020, 514,200 COVID-19 cases, including 29,314 deaths, had been reported in 75.3% (4,196 of 5,570) of municipalities across all five administrative regions of Brazil. TheR(0)value for Brazil was estimated at 3.1 (95% Bayesian credible interval = 2.4-5.5), with a higher median but overlapping credible intervals compared with some other seriously affected countries. A positive association between higher per-capita income and COVID-19 diagnosis was identified. Furthermore, the severe acute respiratory infection cases with unknown aetiology were associated with lower per-capita income. Co-circulation of six respiratory viruses was detected but at very low levels. These findings provide a comprehensive description of the ongoing COVID-19 epidemic in Brazil and may help to guide subsequent measures to control virus transmission.
- Declining antibody levels to Trypanosoma cruzi correlate with polymerase chain reaction positivity and electrocardiographic changes in a retrospective cohort of untreated Brazilian blood donors(2020) BUSS, Lewis F.; SILVA, Lea Campos de Oliveira-da; MOREIRA, Carlos H. V.; MANULI, Erika R.; SALES, Flavia C.; MORALES, Ingra; GERMANIO, Clara Di; ALMEIDA-NETO, Cesar de; BAKKOUR, Sonia; CONSTABLE, Paul; PINTO-FILHO, Marcelo M.; RIBEIRO, Antonio L.; BUSCH, Michael; SABINO, Ester C.Author summary Infection with the single-celled parasite Trypanosoma cruzi (Chagas disease) is thought to be lifelong. However, only a third of infected people develop Chagas cardiomyopathy-the main disease manifestation. This may reflect the different extent to which individuals control the parasite, with some potentially clearing it entirely. In chronically infected immunocompetent patients, a marker of parasite burden is the quantity of antibody against T. cruzi in the blood: more parasite, more immune stimulation, more antibody. In this study we show how antibody levels change over many years in a cohort of untreated patients with Chagas disease. We find that among individuals with falling or low/borderline antibody levels there was a lower rate of parasite detection in the blood and a lower rate of cardiomyopathy. 60% of subjects with falling antibody levels had no evidence of active disease, twice as many as among patients with other antibody trajectories (stable or rising). Our findings support an account of the natural history of Chagas disease in which a proportion of those infected achieve a greater control of the parasite, with some individuals potentially clearing it completely. Background Although infection with Trypanosoma cruzi is thought to be lifelong, less than half of those infected develop cardiomyopathy, suggesting greater parasite control or even clearance. Antibody levels appear to correlate with T. cruzi (antigen) load. We test the association between a downwards antibody trajectory, PCR positivity and ECG alterations in untreated individuals with Chagas disease. Methodology/Principal findings This is a retrospective cohort of T. cruzi seropositive blood donors. Paired blood samples (index donation and follow-up) were tested using the VITROS Immunodiagnostic Products Anti-T.cruzi (Chagas) assay (Ortho Clinical Diagnostics, Raritan NJ) and PCR performed on the follow-up sample. A 12-lead resting ECG was performed. Significant antibody decline was defined as a reduction of > 1 signal-to-cutoff (S/CO) unit on the VITROS assay. Follow-up S/CO of < 4 was defined as borderline/low. 276 untreated seropositive blood donors were included. The median (IQR) follow-up was 12.7 years (8.5-16.9). 56 (22.1%) subjects had a significant antibody decline and 35 (12.7%) had a low/borderline follow-up result. PCR positivity was lower in the falling (26.8% vs 52.8%, p = 0.001) and low/borderline (17.1% vs 51.9%, p < 0.001) antibody groups, as was the rate of ECG abnormalities. Falling and low/borderline antibody groups were predominantly composed of individuals with negative PCR and normal ECG findings: 64% and 71%, respectively. Conclusions/Significance Low and falling antibody levels define a phenotype of possible spontaneous parasite clearance.
- Evolution and epidemic spread of SARS-CoV-2 in Brazil(2020) CANDIDO, Darlan S.; CLARO, Ingra M.; JESUS, Jaqueline G. de; SOUZA, William M.; MOREIRA, Filipe R. R.; DELLICOUR, Simon; MELLAN, Thomas A.; PLESSIS, Louis du; PEREIRA, Rafael H. M.; SALES, Flavia C. S.; MANULI, Erika R.; THEZE, Julien; ALMEIDA, Luiz; MENEZES, Mariane T.; VOLOCH, Carolina M.; FUMAGALLI, Marcilio J.; COLETTI, Thais M.; SILVA, Camila A. M.; RAMUNDO, Mariana S.; AMORIM, Mariene R.; HOELTGEBAUM, Henrique H.; MISHRA, Swapnil; GILL, Mandev S.; CARVALHO, Luiz M.; BUSS, Lewis F.; JR, Carlos A. Prete; ASHWORTH, Jordan; I, Helder Nakaya; PEIXOTO, Pedro S.; BRADY, Oliver J.; NICHOLLS, Samuel M.; TANURI, Amilcar; ROSSI, Atila D.; V, Carlos K. Braga; GERBER, Alexandra L.; GUIMARAES, Ana Paula de C.; JR, Nelson Gaburo; ALENCAR, Cecila Salete; FERREIRA, Alessandro C. S.; LIMA, Cristiano X.; LEVI, Jose Eduardo; GRANATO, Celso; FERREIRA, Giulia M.; JR, Ronaldo S. Francisco; GRANJA, Fabiana; GARCIA, Marcia T.; MORETTI, Maria Luiza; JR, Mauricio W. Perroud; CASTINEIRAS, Terezinha M. P. P.; LAZARI, Carolina S.; HILL, Sarah C.; SANTOS, Andreza Aruska de Souza; SIMEONI, Camila L.; FORATO, Julia; SPOSITO, Andrei C.; SCHREIBER, Angelica Z.; SANTOS, Magnun N. N.; SA, Camila Zolini de; SOUZA, Renan P.; RESENDE-MOREIRA, Luciana C.; TEIXEIRA, Mauro M.; HUBNER, Josy; LEME, Patricia A. F.; MOREIRA, Rennan G.; NOGUEIRA, Mauricio L.; FERGUSON, Neil M.; COSTA, Silvia F.; PROENCA-MODENA, Jose Luiz; VASCONCELOS, Ana Tereza R.; BHATT, Samir; LEMEY, Philippe; WU, Chieh-Hsi; RAMBAUT, Andrew; LOMAN, Nick J.; AGUIAR, Renato S.; PYBUS, Oliver G.; SABINO, Ester C.; FARIA, Nuno RodriguesBrazil currently has one of the fastest-growing severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) epidemics in the world. Because of limited available data, assessments of the impact of nonpharmaceutical interventions (NPIs) on this virus spread remain challenging. Using a mobility-driven transmission model, we show that NPIs reduced the reproduction number from >3 to 1 to 1.6 in Sao Paulo and Rio de Janeiro. Sequencing of 427 new genomes and analysis of a geographically representative genomic dataset identified >100 international virus introductions in Brazil. We estimate that most (76%) of the Brazilian strains fell in three clades that were introduced from Europe between 22 February and 11 March 2020. During the early epidemic phase, we found that SARS-CoV-2 spread mostly locally and within state borders. After this period, despite sharp decreases in air travel, we estimated multiple exportations from large urban centers that coincided with a 25% increase in average traveled distances in national flights. This study sheds new light on the epidemic transmission and evolutionary trajectories of SARS-CoV-2 lineages in Brazil and provides evidence that current interventions remain insufficient to keep virus transmission under control in this country.