VERA LUIZA CAPELOZZI
Projetos de Pesquisa
Unidades Organizacionais
Departamento de Patologia, Faculdade de Medicina - Docente
LIM/03 - Laboratório de Medicina Laboratorial, Hospital das Clínicas, Faculdade de Medicina
LIM/03 - Laboratório de Medicina Laboratorial, Hospital das Clínicas, Faculdade de Medicina
196 resultados
Resultados de Busca
Agora exibindo 1 - 10 de 196
conferenceObject In Situ Evidence Of Pulmonary Endothelial Activation In Patients With Granulomatosis With Polyangiitis And Systemic Sclerosis(2015) KAWANO-DOURADO, L.; AB'SABER, A. M.; CAPELOZZI, V.; BARBAS, C. S. V.conferenceObject MODELING INFLAMMATORY MEDIATORS AND APOPTOSIS BY PLEURAL MESOTHELIAL CELLS EXPOSED TO TALC PARTICLES(2014) ACENCIO, Milena Marques Pagliarelli; TEIXEIRA, Lisete Ribeiro; CAPELOZZI, Vera Luiza; SILVA, Bruna Rocha; ALVARENGA, Vanessa Adelia; SILVA, Carlos Rocha; VARGAS, Francisco Suso; MARCHI, EvaldoconferenceObject Proliferative biomarkers in Idiopathic pulmonary fibrosis: Clinical, radiological and functional significance(2012) PARRA, E. R.; CORNATI, M.; CAPELOZZI, V. L.Introduction: The natural course of idiopathic pulmonary fibrosis (IPF) could be predicted by proliferative markers of the fibrotic process, such as myofibroblasts and interleukins (IL)-13 and IL14. Our primary aim was to determine whether these proliferative markers influence the course of IPF measured by a radiological/functional score. Materials and Methods: Twenty-eight patients with biopsy-proven IPF, who underwent pulmonary evaluation by high-resolution computed tomography (HRCT) fibrosis score and pulmonary function tests, were included in the study. Five normal lung tissues (NLT) were included. Biomarkers in lung tissue were detected by immuno-histochemistry and quantified by histomorphometry for myofibroblasts including alpha-smooth muscle actin (a-SMA), anti-interleukin (IL)-4 and IL-13. Results: Myofibrobalst amount, IL-4 and IL-13 expression were higher in IPF than in NLT (P < 0.01). Myofibroblast expression of a-SMA was positively correlated to IL-14 and IL-13 expression. Lung tissue from patients with high HRCT fibrosis scores expressed significantly greatera-SMA+, IL-4 and IL-13 when compared to patients with low HRCT fibrosis scores (P < 0.05). Negative correlations were found between myofibroblasta-SMA+, vital capacity and diffusing capacity of the lung for carbon monoxide. Conclusions: Proliferative markers, detected by immunohistochemistry, in lung tissue allowed the recognition of a dichotomous distribution of HRCT fibrosis course and influenced pulmonary function tests, suggesting that they may be promising markers of prognosis in these patients.conferenceObject Time-Controlled Adaptive Ventilation Versus Volume-Controlled Ventilation Under Similar Levels of Positive End-Release Pressure and Mean Respiratory System Pressure in Experimental Pneumonia(2020) SILVA, P. L.; MAGALHAES, R. F.; CRUZ, D. G.; ANTUNES, M. A.; FERNANDES, M. V. S.; OLIVEIRA, M. V. de; BRAGA, C. L.; SATALIN, J.; ANDREWS, P.; HABASHI, N.; NIEMAN, G.; GONGALVES-DE-ALBUQUERQUE, C.; SILVA, A. R.; RIBEIRO, R. V.; CAPELOZZI, V. L.; CRUZ, F. F.; SAMARY, C. S.; ROCCO, P. R.conferenceObject Increased decorin and type V collagen in SSc pulmonary fibrosis(2012) TEODORO, W.; VELOSA, A. P.; MARCELINO, A.; MARTIN, P.; CARRASCO, S.; GOLDENSTEIN-SCHAINBERG, C.; PARRA, E.; YOSHINARI, N.; CAPELOZZI, V.Objective: To evaluate COL V and decorin expression in pulmonary tissue and to characterize biochemical profile of COLV from lung fibroblasts culture from SSc patients. Method: We evaluated COL V and decorin expression and tridimensional reconstruction (3D) of 6 patients with SSc without pulmonary hypertension that underwent surgical lung biopsy and as control was obtained lung fragments from 6 normal individuals who died from trauma. COL V amount in lung sections was evaluated with immunofluorescence. To biochemical characterization of COL V from lung fibroblasts culture was used quantitative immunoblot. Results: It was found that the structure of COLV fibers was distorted and strongly thickened in lung tissue from SSc patients compared with thin fibers pattern in the healthy controls. Decorin was distributed around COL V fibrils in the bronchovascular interstitium and vascular walls. Histomorphometric analysis of SSc lung demonstrated increased expression of both COL V and decorin when compared to the control (p<0.01). The semiquantitative imunoblot detected an increased high molecular weight COLV fraction in patients when compared to the control. Conclusion: The over expression and unusual organization of COLV fibers with biochemical changes associated to increased decorin indicates that matrix signalization pathway is involved in COLV fibrillogenesis process in SSc pulmonary fibrosis.conferenceObject Intrapleural Target Therapies (anti-VEGF And Anti-EGFR) Reduce Malignant Pleural Effusion And Morbidity In An Experimental Model(2016) TEIXEIRA, L. R.; ACENCIO, M. M. P.; ALVARENGA, V. A.; PUKA, J.; LEITE, H. F. Z.; MILSONI, P. F.; FERNEZLIAN, S. M.; SANTOS, A. B. G.; MEDEIROS, M. C. R.; MARCHI, E.; CAPELOZZI, V. L.conferenceObject Combining Inhibitor of DNA - Binding Proteins and Angigenic Markers Expression Predict Long Term Survival of Patients with Non-Small Cell Lung Cancer(2013) ANTONANGELO, L.; TUMA, T. S.; VARGAS, F. S.; PARRA, E. R.; TERRA, R. M.; ACENCIO, M.; CAPELOZZI, V. L.Background: Inhibitor of DNA binding (Id) proteins is an emerging promise as biologic marker on oncogenic transformation, cancer progression and tumor angiogenesis, the last, by the regulation of vascular endothelial growth factor (VEGF) expression. Design: We evaluated Ids (1, 2 and 3), VEGF expression and microvessel density (CD34+) in tumor and stromal cells and their impact on survival of 85 patients with surgically excised lung squamous cell carcinoma and adenocarcinoma. Immunohistochemistry and morphometry were used for the quantitation and Kaplan-Meyer survival curves and Cox regression for the statistical analyses. Results: It was found that high Id-1 and VEGF expression and high microvessel density were associated with worse prognosis (Log Rank Test, p<0.001). The Cox model controlled for histological type, age, lymph node stage, Ids, VEGF and microvessel density demonstrated that age, lymph node stage, Id1 and Id3 expression and vascular density were significantly associated with overall survival. A point at the median for Id1, Id3 and vascular density divided patients into 2 groups of different prognosis. Those with higher expression of Id1, Id3 and vascular density had a higher risk of death than those with lower Id-1, Id-3 and microvessel density. Conclusions: Inhibitor of DNA binding (Id) proteins and vascular density are strongly related to prognosis, suggesting that treatment strategies aimed for preventing high Ids synthesis, or local responses to angiogenesis may have impact on NSLC survival.conferenceObject Pathophysiological features of lung and skin in human SSc and collagen V/C57LB6 mouse model(2018) TEODORO, W. Paganelli Rosolia; VELOSA, A. P. Pereira; QUEIROZ, Z. A. de Jesus; SANTOS, L. Araujo dos; CATANOZI, S.; SANTOS FILHO, A. dos; BUENO, C.; VENDRAMINI, M. Borges Galhardo; FERNEZLIAN, S. de Moraes; EHER, E. Miristene; LOPES, F. Degobbi T. Q. S.; CAPELOZZI, V. L.conferenceObject Collagen V activation and matrix extracellular remodeling mediated pulmonary fibrosis in experimental models of bleomycin and 3-5-di-tert-4-hidroxitoluene(2013) LOPES, Deborah; MARTINS, Vanessa; TEODORO, Walcy; ROGER, Edwin; CAPELOZZI, Vera LuizaconferenceObject Airway-Centered Fibroblastic Foci Contributes to Differential Diagnosis of Bronchiolocentric Interstitial Pneumonitis(2020) FABRO, A. T.; BATAH, S.; ALDA, M.; FELIX, R.; FIGUEIRA, R.; SBRAGIA, L.; MACHADO-RUGOLO, J.; BADDINI-MARTINEZ, J.; SANTOS, M.; CAPELOZZI, V.