MARIA LUIZA NOGUEIRA DIAS GENTA

(Fonte: Lattes)
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Instituto do Câncer do Estado de São Paulo, Hospital das Clínicas, Faculdade de Medicina - Médico
Instituto Central, Hospital das Clínicas, Faculdade de Medicina - Médico
LIM/58 - Laboratório de Ginecologia Estrutural e Molecular, Hospital das Clínicas, Faculdade de Medicina

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  • conferenceObject
    Neoadjuvant chemotherapy and radical surgery versus chemorradiation for stage IB2, IIA2 e IIB cervical cancer: A randomized controlled trial
    (2018) REIS FILHO, P. T. F.; ANDRADE, J. M.; BATISTA, M. P.; SOUSA, C. B.; OLIVEIRA, T. H. G. F.; ARRUDA, G. V.; OLIVEIRA, T. M. G.; GABRIELLI, F.; GENTA, M. L. N. D.; POVEDA, A. M.; DIZ, M. D. P. Estevez; REIS, F. J. Candido Dos
  • article 20 Citação(ões) na Scopus
    High-level of viral genomic diversity in cervical cancers: A Brazilian study on human papillomavirus type 16
    (2015) OLIVEIRA, Cristina Mendes de; BRAVO, Ignacio G.; SOUZA, Nathalia Caroline Santiago e; GENTA, Maria Luiza Nogueira Dias; FREGNANI, Jose Humberto Tavares Guerreiro; TACLA, Maricy; CARVALHO, Jesus Paula; LONGATTO-FILHO, Adhemar; LEVI, Jose Eduardo
    Invasive cervical cancer (ICC) is the third most frequent cancer among women worldwide and is associated with persistent infection by carcinogenic human papillomaviruses (HPVs). The combination of large populations of viral progeny and decades of sustained infection may allow for the generation of intra-patient diversity, in spite of the assumedly low mutation rates of PVs. While the natural history of chronic HPVs infections has been comprehensively described, within-host viral diversity remains largely unexplored. In this study we have applied next generation sequencing to the analysis of intra-host genetic diversity in ten ICC and one condyloma cases associated to single HPV16 infection. We retrieved from all cases near full-length genomic sequences. All samples analyzed contained polymorphic sites, ranging from 3 to 125 polymorphic positions per genome, and the median probability of a viral genome picked at random to be identical to the consensus sequence in the lesion was only 40%. We have also identified two independent putative duplication events in two samples, spanning the L2 and the L1 gene, respectively. Finally, we have identified with good support a chimera of human and viral DNA. We propose that viral diversity generated during HPVs chronic infection may be fueled by innate and adaptive immune pressures. Further research will be needed to understand the dynamics of viral DNA variability, differentially in benign and malignant lesions, as well as in tissues with differential intensity of immune surveillance. Finally, the impact of intralesion viral diversity on the long-term oncogenic potential may deserve closer attention.