MAYSA VIEIRA DE SOUSA
Projetos de Pesquisa
Unidades Organizacionais
Instituto Central, Hospital das Clínicas, Faculdade de Medicina
LIM/18 - Laboratório de Carboidratos e Radioimunoensaios, Hospital das Clínicas, Faculdade de Medicina
LIM/18 - Laboratório de Carboidratos e Radioimunoensaios, Hospital das Clínicas, Faculdade de Medicina
5 resultados
Resultados de Busca
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- Positive effects of football on fitness, lipid profile, and insulin resistance in Brazilian patients with type 2 diabetes(2014) SOUSA, M. V. de; FUKUI, R.; KRUSTRUP, P.; PEREIRA, R. M. R.; SILVA, P. R. S.; RODRIGUES, A. C.; ANDRADE, J. L. de; HERNANDEZ, A. J.; SILVA, M. E. R. daWe evaluated the effects of recreational football training combined with calorie-restricted diet (football+diet) vs calorie-restricted diet alone (diet) on aerobic fitness, lipid profile, and insulin resistance indicators in type 2 diabetes (T2D) patients. Forty-four T2D patients aged 48-68 years (27 females, 17 males) were randomly allocated to the football+diet group (FDG; n=22) or to the diet group (DG; n=22), of whom 19 FDG and 15 DG subjects completed the study. The football training was performed for 3x40min/week for 12 weeks. Dual-energy X-ray absorptiometry scanning, treadmill testing, and fasting blood samplings were performed pre and post-intervention. After 12 weeks, maximal oxygen uptake (VO2max) was elevated (P<0.05) by 10 +/- 4% in FDG but not in DG (-3 +/- 4%, P<0.05). After 12 weeks, reductions in blood triglycerides (0.4 +/- 0.1mmol/L), total cholesterol (0.6 +/- 0.2mmol/L), low-density lipoprotein, and very low-density lipoprotein levels were observed only in FDG. Fat mass decreased (P<0.05) by 3.4 +/- 0.4kg in FDG and 3.7 +/- 0.4kg in DG. The lower (P<0.05) glucagon and homeostatic model assessment of insulin resistance indicated an improvement in insulin sensitivity in FDG. In conclusion, football combined with restricted diet was effective in enhancing VO2max, reducing total cholesterol and triglycerides, and increasing insulin sensitivity, potentially providing better tools for the prevention of T2D complications than diet alone.
- Carbohydrate beverages attenuate bone resorption markers in elite runners(2014) SOUSA, Maysa Vieira de; PEREIRA, Rosa Maria R.; FUKUI, Rosa; CAPARBO, Valeria Falco; SILVA, Maria Elizabeth Rossi daObjective. We evaluated the effects of carbohydrate (CHO) supplementation on markers of bone turnover in elite runners. Design. Twenty-four male runners were randomly assigned to two groups - a CHO and a control (CON) group - using a double-blind design. The participants were submitted to an overload training program (days 1-8), followed by a high-intensity intermittent running protocol (10 x 800 m) on day 9. They received a maltodextrin solution (CHO group) or a placebo solution as the CON equivalent, before, during, and after these protocols. Results. After 8 days of intensive training, baseline levels of osteocalcin (OC) decreased in both CHO and CON groups (before: 28.8 +/- 3.6 and 26.6 +/- 2.4 ng/ml, after: 24.8 +/- 3.0 and 21.9 +/- 1.6 ng/ml, respectively, p < 0.01). On day 9, at 80 min of the recovery period, carboxy-terminal of telopeptide type I collagen (CTX) serum concentration was suppressed in the CHO group (0.3 +/- 0.1 ng/ml) vs. 0.6 +/- 0.0 ng/ml for the CON group (p < 0.01). CHO supplementation was effective in decreasing CTX levels from baseline to recovery (0.5 +/- 0.1 ng/mL to 0.3 +/- 0.1 ng/mL, p < 0.001), while an increase from 0.4 +/- 0.0 ng/mL to 0.6 +/- 0.0 ng/mL (p < 0.001) was observed in the CON group. Conclusion. CHO beverage ingestion attenuated the exercise-induced increase in CTX concentration, suggesting that CHO supplementation is a potential strategy to prevent bone damage in athletes.
- Combination of recreational soccer and caloric restricted diet reduces markers of protein catabolism and cardiovascular risk in patients with type 2 diabetes(2017) SOUSA, M. Vieira De; FUKUI, R.; KRUSTRUP, P.; DAGOGO-JACK, S.; SILVA, M. E. Rossi DaModerate calorie-restricted diets and exercise training prevent loss of lean mass and cardiovascular risk. Because adherence to routine exercise recommendation is generally poor, we utilized recreational soccer training as a novel therapeutic exercise intervention in type 2 diabetes (T2D) patients. We compared the effects of acute and chronic soccer training plus calorie-restricted diet on protein catabolism and cardiovascular risk markers in T2D. Fifty-one T2D patients (61.1 +/- 6.4 years, 29 females: 22 males) were randomly allocated to the soccer+diet-group (SDG) or to the dietgroup (DG). The 40-min soccer sessions were held 3 times per week for 12 weeks. Nineteen participants attended 100% of scheduled soccer sessions, and none suffered any injuries. The SDG group showed higher levels of growth hormone (GH), free fatty acids and ammonia compared with DG. After 12 weeks, insulin-like growth factor binding protein (IGFPB)-3 and glucose levels were lower in SDG, whereas insulin-like growth factor (IGF)-1/ IGFBP-3 ratio increased in both groups. After the last training session, an increase in IGF-1/IGFBP-3 and attenuation in ammonia levels were suggestive of lower muscle protein catabolism. Recreational soccer training was popular and safe, and was associated with decreased plasma glucose and IGFBP-3 levels, decreased ammoniagenesis, and increased lipolytic activity and IGF-1/IGFBP-3 ratio, all indicative of attenuated catabolism.
- Carbohydrate supplementation delays DNA damage in elite runners during intensive microcycle training(2012) SOUSA, Maysa Vieira de; MADSEN, Klavs; FUKUI, Rosa; SANTOS, Aritania; SILVA, Maria Elizabeth Rossi daThe aim of this study was to evaluate the effect of carbohydrate supplementation on free plasma DNA and conventional markers of training and tissue damage in long-distance runners undergoing an overload training program. Twenty-four male runners were randomly assigned to two groups (CHO group and control group). The participants were submitted to an overload training program (days 1-8), followed by a high-intensity intermittent running protocol (10 x 800 m) on day 9. The runners received maltodextrin solution (CHO group) or zero energy placebo solution as the control equivalent before, during, and after this protocol. After 8 days of intensive training, baseline LDH levels remained constant in the CHO group (before: 449.1 +/- 18.2, after: 474.3 +/- 22.8 U/L) and increased in the control group (from 413.5 +/- 23.0 to 501.8 +/- 24.1 U/L, p < 0.05). On day 9, LDH concentrations were lower in the CHO group (509.2 +/- 23.1 U/L) than in the control group (643.3 +/- 32.9 U/L, p < 0.01) post-intermittent running. Carbohydrate ingestion attenuated the increase of free plasma DNA post-intermittent running (48,240.3 +/- 5,431.8 alleles/mL) when compared to the control group (73,751.8 +/- 11,546.6 alleles/mL, p < 0.01). Leukocyte counts were lower in the CHO group than in the control group post-intermittent running (9.1 +/- 0.1 vs. 12.2 +/- 0.7 cells/mu L; p < 0.01) and at 80 min of recovery (10.6 +/- 0.1 vs. 13.9 +/- 1.1 cells/mu L; p < 0.01). Cortisol levels were positively correlated with free plasma DNA, leukocytes, and LDH (all r > 0.4 and p < 0.001). The results showed that ingestion of a carbohydrate beverage resulted in less DNA damage and attenuated the acute post-exercise inflammation response, providing better recovery during intense training.
- Biomarkers of insulin action during single soccer sessions before and after a 12-week training period in type 2 diabetes patients on a caloric-restricted diet(2019) SOUSA, Maysa V. de; FUKUI, Rosa; DAGOGO-JACK, Samuel; KRUSTRUP, Peter; ZOUHAL, Hassane; SILVA, Maria Elizabeth R. daBackground: We investigated the biomarkers of insulin action as well as changes in free fatty acids and lactate concentration after an acute soccer session pre and post training with caloric-restricted diet versus diet alone in type 2 diabetes (T2D) patients. Methods: Fifty-one middle-aged (61.1 +/- 6.4 years) T2D patients were randomly allocated to the soccer + diet group (SDG) or the diet group (DG). The control group comprised T2D patients observing a caloric-restricted diet who did not receive soccer training. Over 12 weeks, SDG performed 3 x 40 min per week of soccer training. Results: The first soccer session for SDG induced acute increases in blood lactate (1.4 +/- 0.1-6.0 +/- 0.7 mmol/l, P < 0.05) and glucagon levels (112.1 +/- 6.2-142.9 +/- 8.0 pg/ml, P < 0.05), whereas glucose and insulin levels remained unchanged. Moreover, this session showed suppressed insulin levels as well as higher free fatty acids, lactate levels and glucagon/insulin ratio compared to DG (p < 0.05). After 12 weeks, a baseline decrease was observed in glucagon, leptin and lactate levels in SDG and DG (p < 0.05), whereas HOMA-IR, Adipo-IR and glucose levels were lower only in SDG (p < 0.05). At the last soccer training session, the blood lactate response was significantly lower than for the first session (4.0 +/- 0.4 vs 6.0 +/- 0.7 mmol/l). At 48 h pre intervention, a decrease was observed in leptin levels (p < 0.05), which remained lower post intervention. The positive correlation between leptin and insulin, and the lower levels after training, could be attributed to the improved insulin sensitivity along with the weight loss observed in both groups (similar to 3.4 kg for DG and 3.7 kg for SDG). Conclusion: Acute soccer sessions markedly improved insulin action markers in T2D patients, while the cumulative effects enhanced insulin sensitivity and decreased risk factors associated with cardiovascular disease after 12 weeks of intervention better than caloric-restricted diet.