MAYSA VIEIRA DE SOUSA
Projetos de Pesquisa
Unidades Organizacionais
Instituto Central, Hospital das Clínicas, Faculdade de Medicina
LIM/18 - Laboratório de Carboidratos e Radioimunoensaios, Hospital das Clínicas, Faculdade de Medicina
LIM/18 - Laboratório de Carboidratos e Radioimunoensaios, Hospital das Clínicas, Faculdade de Medicina
10 resultados
Resultados de Busca
Agora exibindo 1 - 10 de 10
- The ""Football is Medicine"" platformscientific evidence, large-scale implementation of evidence-based concepts and future perspectives(2018) KRUSTRUP, P.; WILLIAMS, C. A.; MOHR, M.; HANSEN, P. R.; HELGE, E. W.; ELBE, A. -M.; SOUSA, M. de; DVORAK, J.; JUNGE, A.; HAMMAMI, A.; HOLTERMANN, A.; LARSEN, M. N.; KIRKENDALL, D.; SCHMIDT, J. F.; ANDERSEN, T. R.; BUONO, P.; RORTH, M.; PARNELL, D.; OTTESEN, L.; BENNIKE, S.; NIELSEN, J. J.; MENDHAM, A. E.; ZAR, A.; UTH, J.; HORNSTRUP, T.; BRASSO, K.; NYBO, L.; KRUSTRUP, B. R.; MEYER, T.; AAGAARD, P.; ANDERSEN, J. L.; HUBBALL, H.; REDDY, P. A.; RYOM, K.; LOBELO, F.; BARENE, S.; HELGE, J. W.; FATOUROS, I. G.; NASSIS, G. P.; XU, J. C.; PETTERSEN, S. A.; CALBET, J. A.; SEABRA, A.; REBELO, A. N.; FIGUEIREDO, P.; POVOAS, S.; CASTAGNA, C.; MILANOVIC, Z.; BANGSBO, J.; RANDERS, M. B.; BRITO, J.
- Positive effects of football on fitness, lipid profile, and insulin resistance in Brazilian patients with type 2 diabetes(2014) SOUSA, M. V. de; FUKUI, R.; KRUSTRUP, P.; PEREIRA, R. M. R.; SILVA, P. R. S.; RODRIGUES, A. C.; ANDRADE, J. L. de; HERNANDEZ, A. J.; SILVA, M. E. R. daWe evaluated the effects of recreational football training combined with calorie-restricted diet (football+diet) vs calorie-restricted diet alone (diet) on aerobic fitness, lipid profile, and insulin resistance indicators in type 2 diabetes (T2D) patients. Forty-four T2D patients aged 48-68 years (27 females, 17 males) were randomly allocated to the football+diet group (FDG; n=22) or to the diet group (DG; n=22), of whom 19 FDG and 15 DG subjects completed the study. The football training was performed for 3x40min/week for 12 weeks. Dual-energy X-ray absorptiometry scanning, treadmill testing, and fasting blood samplings were performed pre and post-intervention. After 12 weeks, maximal oxygen uptake (VO2max) was elevated (P<0.05) by 10 +/- 4% in FDG but not in DG (-3 +/- 4%, P<0.05). After 12 weeks, reductions in blood triglycerides (0.4 +/- 0.1mmol/L), total cholesterol (0.6 +/- 0.2mmol/L), low-density lipoprotein, and very low-density lipoprotein levels were observed only in FDG. Fat mass decreased (P<0.05) by 3.4 +/- 0.4kg in FDG and 3.7 +/- 0.4kg in DG. The lower (P<0.05) glucagon and homeostatic model assessment of insulin resistance indicated an improvement in insulin sensitivity in FDG. In conclusion, football combined with restricted diet was effective in enhancing VO2max, reducing total cholesterol and triglycerides, and increasing insulin sensitivity, potentially providing better tools for the prevention of T2D complications than diet alone.
- Skeletal muscle gene expression in older adults with type 2 diabetes mellitus undergoing calorie-restricted diet and recreational sports training - a randomized clinical trial(2022) SOARES, Diana Bento da Silva; SHINJO, Samuel Katsuyuki; SANTOS, Aritania Sousa; JESUS, Joyce de Cassia Rosa de; SCHENK, Simon; CASTRO, Gabriela Salim de; ZANOTELI, Edmar; KRUSTRUP, Peter; SILVA, Maria Elizabeth Rossi da; SOUSA, Maysa Vieira deAims: This study aimed to evaluate the impact of a 12-week calorie-restricted diet and recreational sports training on gene expressions IL-15, ATROGIN-1 and MURF-1 in skeletal muscle of T2D patients. Methods: Older adults with T2D (n = 39, 60 +/- 6.0 years, BMI 33.5 +/- 0.6 kg/m(2)) were randomly allocated to Diet+Soccer (DS), Diet+Running (DR) or Diet (D). The training sessions were moderate-to-high-intensity and performed 3 x 40 min/week for 12-weeks. Gene expression from vastus lateralis muscle obtained by qRT-PCR, dual-energy X-ray and fasting blood testing measurements were performed before and after 12-weeks. Statistical analysis adopted were two-way ANOVA and Paired t-test for gene expression, and RM-ANOVA test for the remainder variables. Results: Total body weight was reduced in similar to 4 kg representing body fat mass in all groups after 12-weeks (P < 0.05). HbA1c values decreased in all groups post-intervention. Lipids profile improved in the training groups (P < 0.05) after 12-weeks. ATROGIN-1 and MURF-1 mRNA reduced in the DS (1.084 +/- 0.14 vs. 0.754 +/- 1.14 and 1.175 +/- 0.34 vs. 0.693 +/- 0.12, respectively; P < 0.05), while IL-15 mRNA increased in the DR (1.056 +/- 0.12 vs. 1.308 +/- 0.13; P < 0.05) after 12-weeks intervention. Conclusion: Recreational training with a moderate calorie-restricted diet can downregulates the expression of atrophy-associated myokines and increases the expression of anti-inflammatory gene IL-15.
- Combination of recreational soccer and caloric restricted diet reduces markers of protein catabolism and cardiovascular risk in patients with type 2 diabetes(2017) SOUSA, M. Vieira De; FUKUI, R.; KRUSTRUP, P.; DAGOGO-JACK, S.; SILVA, M. E. Rossi DaModerate calorie-restricted diets and exercise training prevent loss of lean mass and cardiovascular risk. Because adherence to routine exercise recommendation is generally poor, we utilized recreational soccer training as a novel therapeutic exercise intervention in type 2 diabetes (T2D) patients. We compared the effects of acute and chronic soccer training plus calorie-restricted diet on protein catabolism and cardiovascular risk markers in T2D. Fifty-one T2D patients (61.1 +/- 6.4 years, 29 females: 22 males) were randomly allocated to the soccer+diet-group (SDG) or to the dietgroup (DG). The 40-min soccer sessions were held 3 times per week for 12 weeks. Nineteen participants attended 100% of scheduled soccer sessions, and none suffered any injuries. The SDG group showed higher levels of growth hormone (GH), free fatty acids and ammonia compared with DG. After 12 weeks, insulin-like growth factor binding protein (IGFPB)-3 and glucose levels were lower in SDG, whereas insulin-like growth factor (IGF)-1/ IGFBP-3 ratio increased in both groups. After the last training session, an increase in IGF-1/IGFBP-3 and attenuation in ammonia levels were suggestive of lower muscle protein catabolism. Recreational soccer training was popular and safe, and was associated with decreased plasma glucose and IGFBP-3 levels, decreased ammoniagenesis, and increased lipolytic activity and IGF-1/IGFBP-3 ratio, all indicative of attenuated catabolism.
- Executive summary: Elite women's football-Performance, recovery, diet, and health(2022) MOHR, Magni; BRITO, Joao; SOUSA, Maysa de; PETTERSEN, Svein ArneThe present special issue of Scandinavian Journal of Medicine & Science in Sports focuses on performance, recovery, diet, and health in elite women's football. Beside this summary, an editorial, topic reviews, and original articles written by several of the most published authors in football research are presented. It is, for example, highlighted that there is a great gender inequality in football research in favor of men, especially within elite football populations. Therefore, the broad-spectrum content of the special issue with focus on several performance areas in women's football, recovery strategies, nutrition, and psychological factors is highly warranted. Several of the topics examined and data presented are examined for the first on elite women's football, and therefore, we hope that this special issue will contribute to gender balance the research and emphasis on football in both genders.
- Carbohydrate supplementation delays DNA damage in elite runners during intensive microcycle training(2012) SOUSA, Maysa Vieira de; MADSEN, Klavs; FUKUI, Rosa; SANTOS, Aritania; SILVA, Maria Elizabeth Rossi daThe aim of this study was to evaluate the effect of carbohydrate supplementation on free plasma DNA and conventional markers of training and tissue damage in long-distance runners undergoing an overload training program. Twenty-four male runners were randomly assigned to two groups (CHO group and control group). The participants were submitted to an overload training program (days 1-8), followed by a high-intensity intermittent running protocol (10 x 800 m) on day 9. The runners received maltodextrin solution (CHO group) or zero energy placebo solution as the control equivalent before, during, and after this protocol. After 8 days of intensive training, baseline LDH levels remained constant in the CHO group (before: 449.1 +/- 18.2, after: 474.3 +/- 22.8 U/L) and increased in the control group (from 413.5 +/- 23.0 to 501.8 +/- 24.1 U/L, p < 0.05). On day 9, LDH concentrations were lower in the CHO group (509.2 +/- 23.1 U/L) than in the control group (643.3 +/- 32.9 U/L, p < 0.01) post-intermittent running. Carbohydrate ingestion attenuated the increase of free plasma DNA post-intermittent running (48,240.3 +/- 5,431.8 alleles/mL) when compared to the control group (73,751.8 +/- 11,546.6 alleles/mL, p < 0.01). Leukocyte counts were lower in the CHO group than in the control group post-intermittent running (9.1 +/- 0.1 vs. 12.2 +/- 0.7 cells/mu L; p < 0.01) and at 80 min of recovery (10.6 +/- 0.1 vs. 13.9 +/- 1.1 cells/mu L; p < 0.01). Cortisol levels were positively correlated with free plasma DNA, leukocytes, and LDH (all r > 0.4 and p < 0.001). The results showed that ingestion of a carbohydrate beverage resulted in less DNA damage and attenuated the acute post-exercise inflammation response, providing better recovery during intense training.
- Effects of recreational football on women's fitness and health: adaptations and mechanisms(2018) KRUSTRUP, Peter; HELGE, Eva Wulff; HANSEN, Peter R.; AAGAARD, Per; HAGMAN, Marie; RANDERS, Morten B.; SOUSA, Maysa de; MOHR, MagniThe review describes the fitness and health effects of recreational football in women aged 18-65 years. The review documents that 2 x 1 h of recreational football training for 12-16 weeks causes marked improvements in maximal oxygen uptake (5-15%) and myocardial function in women. Moreover, mean arterial blood pressure was shown to decrease by 2-5 mmHg in normotensive women and 6-8 mmHg in hypertensive women. This review also show that short-term (< 4 months) and medium-term (4-16 months) recreational football training has major beneficial impact on metabolic health profile in women, with fat losses of 1-3 kg and improvements in blood lipid profile. Lastly, 2 x 1 h per week of recreational football training for women elevates lower extremity bone mineralisation by 1-5% and whole-body bone mineralization by 1-2% within 4-12-month interventions. These training adaptations are related to the high heart rates, high number of fast runs, and multiple changes of direction and speed occurring during recreational football training for untrained women. In conclusion, regular small-sided football training for women is an intense and versatile type of training that combines elements of high-intensity interval training (HIIT), endurance training and strength training, thereby providing optimal stimuli for cardiovascular, metabolic and musculoskeletal fitness. Recreational football, therefore, seems to be an effective tool for prevention and treatment of lifestyle diseases in young and middle-aged women, including hypertension, type 2 diabetes and osteopenia. Future research should elucidate effects of football training for elderly women, and as treatment and rehabilitation of breast cancer patients and other women patient groups.
- Carbohydrate supplementation increases intramyocellular lipid stores in elite runners(2012) SOUSA, Maysa; SIMOES, Herbert Gustavo; CASTRO, Claudio Campi de; OTADUY, Maria Concepcion Garcia; NEGRAO, Carlos Eduardo; PEREIRA, Rosa Maria Rodrigues; MADSEN, Klaus; SILVA, Maria Elizabeth Rossi daThe objective was to determine the effects of carbohydrate (CHO) supplementation on exercise-induced hormone responses and post-training intramyocellular lipid stores (IMCL). Twenty-four elite male athletes (28.0 +/- 1.2 years) were randomized to receive CHO (maltodextrin solution) or zero energy placebo solution (control group). The high-intensity running protocol consisted of 10 x 800 m at 100% of the best 3000-m speed (Vm3 km) and 2 x 1000 m maximal bouts in the morning and a submaximal 10-km continuous easy running in the afternoon of day 9. IMCL concentrations were assessed by H-1-MRS before (-day 9) and after training (day 9) in soleus (SO) and tibialis anterior (TA) muscles. Blood hormones were also measured before, during, and post-exercise. The percent change (Delta%) in TA-IMCL was higher in the CHO group (47.9 +/- 24.5 IMCL/Cr) than in the control group (-1.7 +/- 13.1, respectively) (P=.04). Insulin concentrations were higher in the CHO group post-intermittent running compared to control (P=.02). Circulating levels of free fatty acids and GH were lower in the CHO group (P>.01). The decline in performance in the 2nd 1000-m bout was also attenuated in this group compared to control (P<.001 and P=.0035, respectively). The hormonal milieu (higher insulin and lower GH levels) in the CHO group, together with unchanged free fatty acid levels, probably contributed to the increased IMCL stores. This greater energy storage capacity may have improved post-exercise recovery and thus prevented performance deterioration.
- Nutritional optimization for female elite football players-topical review(2022) V, Maysa de Sousa; LUNDSGAARD, Anne-Marie; CHRISTENSEN, Peter M.; CHRISTENSEN, Lars; RANDERS, Morten B.; MOHR, Magni; NYBO, Lars; KIENS, Bente; FRITZEN, Andreas M.Women's football is an intermittent sport characterized by frequent intense actions throughout the match. The high number of matches with limited recovery time played across a long competitive season underlines the importance of nutritional strategies to meet these large physical demands. In order to maximize sport performance and maintain good health, energy intake must be optimal. However, a considerable proportion of female elite football players does not have sufficient energy intake to match the energy expenditure, resulting in low energy availability that might have detrimental physiologic consequences and impair performance. Carbohydrates appear to be the primary fuel covering the total energy supply during match-play, and female elite football players should aim to consume sufficient carbohydrates to meet the requirements of their training program and to optimize the replenishment of muscle glycogen stores between training bouts and matches. However, several macro- and micronutrients are important for ensuring sufficient energy and nutrients for performance optimization and for overall health status in female elite football players. The inadequacy of macro-and micronutrients in the diet of these athletes may impair performance and training adaptations, and increase the risk of health disorders, compromising the player's professional career. In this topical review, we present knowledge and relevant nutritional recommendations for elite female football players for the benefit of sports nutritionists, dietitians, sports scientists, healthcare specialists, and applied researchers. We focus on dietary intake and cover the most pertinent topics in sports nutrition for the relevant physical demands in female elite football players as follows: energy intake, macronutrient and micronutrient requirements and optimal composition of the everyday diet, nutritional and hydration strategies to optimize performance and recovery, potential ergogenic effects of authorized relevant supplements, and future research considerations.
- Biomarkers of insulin action during single soccer sessions before and after a 12-week training period in type 2 diabetes patients on a caloric-restricted diet(2019) SOUSA, Maysa V. de; FUKUI, Rosa; DAGOGO-JACK, Samuel; KRUSTRUP, Peter; ZOUHAL, Hassane; SILVA, Maria Elizabeth R. daBackground: We investigated the biomarkers of insulin action as well as changes in free fatty acids and lactate concentration after an acute soccer session pre and post training with caloric-restricted diet versus diet alone in type 2 diabetes (T2D) patients. Methods: Fifty-one middle-aged (61.1 +/- 6.4 years) T2D patients were randomly allocated to the soccer + diet group (SDG) or the diet group (DG). The control group comprised T2D patients observing a caloric-restricted diet who did not receive soccer training. Over 12 weeks, SDG performed 3 x 40 min per week of soccer training. Results: The first soccer session for SDG induced acute increases in blood lactate (1.4 +/- 0.1-6.0 +/- 0.7 mmol/l, P < 0.05) and glucagon levels (112.1 +/- 6.2-142.9 +/- 8.0 pg/ml, P < 0.05), whereas glucose and insulin levels remained unchanged. Moreover, this session showed suppressed insulin levels as well as higher free fatty acids, lactate levels and glucagon/insulin ratio compared to DG (p < 0.05). After 12 weeks, a baseline decrease was observed in glucagon, leptin and lactate levels in SDG and DG (p < 0.05), whereas HOMA-IR, Adipo-IR and glucose levels were lower only in SDG (p < 0.05). At the last soccer training session, the blood lactate response was significantly lower than for the first session (4.0 +/- 0.4 vs 6.0 +/- 0.7 mmol/l). At 48 h pre intervention, a decrease was observed in leptin levels (p < 0.05), which remained lower post intervention. The positive correlation between leptin and insulin, and the lower levels after training, could be attributed to the improved insulin sensitivity along with the weight loss observed in both groups (similar to 3.4 kg for DG and 3.7 kg for SDG). Conclusion: Acute soccer sessions markedly improved insulin action markers in T2D patients, while the cumulative effects enhanced insulin sensitivity and decreased risk factors associated with cardiovascular disease after 12 weeks of intervention better than caloric-restricted diet.