MicroRNA 100: a context dependent miRNA in prostate cancer

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dc.contributorSistema FMUSP-HC: Faculdade de Medicina da Universidade de São Paulo (FMUSP) e Hospital das Clínicas da FMUSP
dc.contributor.authorLEITE, Katia R. M.
dc.contributor.authorMORAIS, Denis R.
dc.contributor.authorREIS, Sabrina T.
dc.contributor.authorVIANA, Nayara
dc.contributor.authorMOURA, Caio
dc.contributor.authorFLOREZ, Manuel Garcia
dc.contributor.authorSILVA, Iran A.
dc.contributor.authorDIP, Nelson
dc.contributor.authorSROUGI, Miguel
dc.date.accessioned2013-09-23T16:30:04Z
dc.date.available2013-09-23T16:30:04Z
dc.date.issued2013
dc.description.abstractOBJECTIVE: MicroRNAs are noncoding RNA molecules involved in the development and progression of tumors. We have found that miRNA-100 is underexpressed in metastatic prostate cancer compared to localized disease. Conversely higher levels of miR-100 are related to biochemical recurrence after surgery. This suggests that miR-100 may be a context-dependent miRNA, acting as oncogene or tumor suppressor miRNA. Our aim is to demonstrate the role of miR-100 in the control of predicted target genes in prostate cancer cell lines. METHODS: Cell lines DU145 and PC3 were transfected with miR-100, antimiR-100 and after 24 h and 48 h of exposure, qRT-PCR and western blot were performed for mTOR, FGFR3, THAP2, SMARCA5 and BAZ2A. RESULTS: There was reduction in mTOR (p = 0.025), THAP2 (p = 0.038), SMARCA5 (p = 0.001) and BAZ2A (p = 0.006) mRNA expression in DU145 cells after exposure to miR-100. In PC3 cells, mTOR expression was decreased by miR-100 (p = 0.01). There was a reduction in the expression levels of proteins encoded by studied genes, ranging from 34% to 69%. CONCLUSIONS: We demonstrate that miR-100 is a context-dependent miRNA controlling BAZ2, mTOR, FGFR3, SMARCA5 and THAP2 that might be involved in PC progression. The elucidation of the roles of miRNAs in tumors is important because they can be used as therapeutic targets in the future.
dc.description.indexMEDLINE
dc.description.sponsorshipFAPESP [10/51207-6]
dc.identifier.citationCLINICS, v.68, n.6, p.797-802, 2013
dc.identifier.doi10.6061/clinics/2013(06)12
dc.identifier.issn1807-5932
dc.identifier.urihttps://observatorio.fm.usp.br/handle/OPI/1680
dc.language.isoeng
dc.publisherHOSPITAL CLINICAS, UNIV SAO PAULO
dc.relation.ispartofClinics
dc.rightsopenAccess
dc.rights.holderCopyright HOSPITAL CLINICAS, UNIV SAO PAULO
dc.subjectProstate Cancer
dc.subjectMicro RNA
dc.subjectmiR-100
dc.subjectGene Expression
dc.subjectProtein Expression
dc.subjectPCR
dc.subjectWestern Blot
dc.subject.otherchromatin
dc.subject.otherpathways
dc.subject.wosMedicine, General & Internal
dc.titleMicroRNA 100: a context dependent miRNA in prostate cancer
dc.typearticle
dc.type.categoryoriginal article
dc.type.versionpublishedVersion
dspace.entity.typePublication
hcfmusp.citation.scopus31
hcfmusp.contributor.author-fmusphcKATIA RAMOS MOREIRA LEITE
hcfmusp.contributor.author-fmusphcDENIS REIS MORAIS
hcfmusp.contributor.author-fmusphcSABRINA THALITA DOS REIS FARIA
hcfmusp.contributor.author-fmusphcNAYARA IZABEL VIANA MOURA
hcfmusp.contributor.author-fmusphcCAIO MARTINS MOURA
hcfmusp.contributor.author-fmusphcMANUEL GARCIA FLOREZ
hcfmusp.contributor.author-fmusphcIRAN AMORIM DA SILVA
hcfmusp.contributor.author-fmusphcNELSON GASPAR DIP JUNIOR
hcfmusp.contributor.author-fmusphcMIGUEL SROUGI
hcfmusp.description.beginpage797
hcfmusp.description.endpage802
hcfmusp.description.issue6
hcfmusp.description.volume68
hcfmusp.origemWOS
hcfmusp.origem.pubmed23778488
hcfmusp.origem.scieloSCIELO:S1807-59322013000600797
hcfmusp.origem.scopus2-s2.0-84879288418
hcfmusp.origem.wosWOS:000320463800012
hcfmusp.publisher.citySAO PAULO
hcfmusp.publisher.countryBRAZIL
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hcfmusp.scopus.lastupdate2024-05-17
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