ENGRAFTMENT OF HUMAN ADIPOSE DERIVED STEM CELLS DELIVERED IN A HYALURONIC ACID PREPARATION IN MICE

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dc.contributorSistema FMUSP-HC: Faculdade de Medicina da Universidade de São Paulo (FMUSP) e Hospital das Clínicas da FMUSP
dc.contributor.authorDIETRICH, Isa
dc.contributor.authorCOCHET, Olivia
dc.contributor.authorVILLAGEOIS, Phi
dc.contributor.authorRODRIGUES, Consuelo Junqueira
dc.date.accessioned2013-10-11T21:24:24Z
dc.date.available2013-10-11T21:24:24Z
dc.date.issued2012
dc.description.abstractPurpose: To evaluate the implant of human adipose-derived stem cells (ADSC) delivered in hyaluronic acid gel (HA), injected subcutaneously in athymic mice. Methods: Control implants: HA plus culture media was injected in the subcutaneous of the left subscapular area of 12 athymic mice. ADSC implants: HA plus ADSC suspended in culture media was injected in the subcutaneous, at the contra lateral area, of the same animals. With 8 weeks, animals were sacrificed and the recovered implants were processed for extraction of genomic DNA, and histological study by hematoxylin–eosin staining and immunofluorescence using anti-human vimentin and anti-von Willebrand factor antibodies. Results–Controls: Not visualized at the injection site. An amorphous substance was observed in hematoxylin–eosin stained sections. Human vimentin and anti von Willebrand factor were not detected. No human DNA was detected. ADSC implants-A plug was visible at the site of injection. Fusiform cells were observed in sections stained by hematoxylin-eosin and both human vimentin and anti von Willebrand factor were detected by immunofluorescence. The presence of human DNA was confirmed. Conclusion: The delivery of ADSC in preparations of hyaluronic acid assured cells engraftment at the site of injection.
dc.description.conferencedateOCT 04-05, 2012
dc.description.conferencelocalAthens, GREECE
dc.description.conferencename22nd Annual Meeting of the European Tissue Repair Society
dc.description.indexMEDLINE
dc.identifier.citationWOUND REPAIR AND REGENERATION, v.20, n.5, p.A90-A90, 2012
dc.identifier.issn1067-1927
dc.identifier.urihttps://observatorio.fm.usp.br/handle/OPI/2931
dc.language.isoeng
dc.publisherWILEY-BLACKWELL
dc.relation.ispartofWound Repair and Regeneration
dc.rightsrestrictedAccess
dc.rights.holderCopyright WILEY-BLACKWELL
dc.subject.wosCell Biology
dc.subject.wosDermatology
dc.subject.wosMedicine, Research & Experimental
dc.subject.wosSurgery
dc.titleENGRAFTMENT OF HUMAN ADIPOSE DERIVED STEM CELLS DELIVERED IN A HYALURONIC ACID PREPARATION IN MICE
dc.typeconferenceObject
dc.type.categorymeeting abstract
dc.type.versionpublishedVersion
dspace.entity.typePublication
hcfmusp.author.externalCOCHET, Olivia:Univ Sao Paulo, Dept Surg, Lab Surg Anat Human Struct Topog, Sch Med, Sao Paulo, Brazil
hcfmusp.author.externalVILLAGEOIS, Phi:Univ Sao Paulo, Dept Surg, Lab Surg Anat Human Struct Topog, Sch Med, Sao Paulo, Brazil
hcfmusp.contributor.author-fmusphcISA DIETRICH
hcfmusp.contributor.author-fmusphcCONSUELO JUNQUEIRA RODRIGUES
hcfmusp.description.beginpageA90
hcfmusp.description.endpageA90
hcfmusp.description.issue5
hcfmusp.description.volume2012
hcfmusp.origemWOS
hcfmusp.origem.wosWOS:000308945000136
hcfmusp.publisher.cityHOBOKEN
hcfmusp.publisher.countryUSA
relation.isAuthorOfPublication8660a41f-878f-4358-b413-d661eda42850
relation.isAuthorOfPublicatione0b0e6cd-c00a-4030-bc8b-267a782f27b8
relation.isAuthorOfPublication.latestForDiscoverye0b0e6cd-c00a-4030-bc8b-267a782f27b8
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