Increased decorin and type V collagen in SSc pulmonary fibrosis

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dc.contributorSistema FMUSP-HC: Faculdade de Medicina da Universidade de São Paulo (FMUSP) e Hospital das Clínicas da FMUSP
dc.contributor.authorTEODORO, W.
dc.contributor.authorVELOSA, A. P.
dc.contributor.authorMARCELINO, A.
dc.contributor.authorMARTIN, P.
dc.contributor.authorCARRASCO, S.
dc.contributor.authorGOLDENSTEIN-SCHAINBERG, C.
dc.contributor.authorPARRA, E.
dc.contributor.authorYOSHINARI, N.
dc.contributor.authorCAPELOZZI, V.
dc.date.accessioned2013-10-11T21:16:38Z
dc.date.available2013-10-11T21:16:38Z
dc.date.issued2012
dc.description.abstractObjective: To evaluate COL V and decorin expression in pulmonary tissue and to characterize biochemical profile of COLV from lung fibroblasts culture from SSc patients. Method: We evaluated COL V and decorin expression and tridimensional reconstruction (3D) of 6 patients with SSc without pulmonary hypertension that underwent surgical lung biopsy and as control was obtained lung fragments from 6 normal individuals who died from trauma. COL V amount in lung sections was evaluated with immunofluorescence. To biochemical characterization of COL V from lung fibroblasts culture was used quantitative immunoblot. Results: It was found that the structure of COLV fibers was distorted and strongly thickened in lung tissue from SSc patients compared with thin fibers pattern in the healthy controls. Decorin was distributed around COL V fibrils in the bronchovascular interstitium and vascular walls. Histomorphometric analysis of SSc lung demonstrated increased expression of both COL V and decorin when compared to the control (p<0.01). The semiquantitative imunoblot detected an increased high molecular weight COLV fraction in patients when compared to the control. Conclusion: The over expression and unusual organization of COLV fibers with biochemical changes associated to increased decorin indicates that matrix signalization pathway is involved in COLV fibrillogenesis process in SSc pulmonary fibrosis.
dc.description.conferencedateSEP 08-12, 2012
dc.description.conferencelocalPrague, CZECH REPUBLIC
dc.description.conferencename24th European Congress of Pathology
dc.description.indexMEDLINE
dc.identifier.citationVIRCHOWS ARCHIV, v.461, suppl.1, p.S55-S55, 2012
dc.identifier.issn0945-6317
dc.identifier.urihttps://observatorio.fm.usp.br/handle/OPI/2705
dc.language.isoeng
dc.publisherSPRINGER
dc.relation.ispartofVirchows Archiv
dc.rightsrestrictedAccess
dc.rights.holderCopyright SPRINGER
dc.subject.wosPathology
dc.titleIncreased decorin and type V collagen in SSc pulmonary fibrosis
dc.typeconferenceObject
dc.type.categorymeeting abstract
dc.type.versionpublishedVersion
dspace.entity.typePublication
hcfmusp.contributor.author-fmusphcWALCY PAGANELLI ROSOLIA TEODORO
hcfmusp.contributor.author-fmusphcANA PAULA PEREIRA VELOSA
hcfmusp.contributor.author-fmusphcPATRICIA MARTIN
hcfmusp.contributor.author-fmusphcSOLANGE CARRASCO
hcfmusp.contributor.author-fmusphcCLAUDIA GOLDENSTEIN SCHAINBERG
hcfmusp.contributor.author-fmusphcEDWIN ROGER PARRA CUENTAS
hcfmusp.contributor.author-fmusphcNATALINO HAJIME YOSHINARI
hcfmusp.contributor.author-fmusphcVERA LUIZA CAPELOZZI
hcfmusp.description.beginpageS55
hcfmusp.description.endpageS55
hcfmusp.description.issuesuppl 1
hcfmusp.description.volume461
hcfmusp.origemWOS
hcfmusp.origem.wosWOS:000310364801154
hcfmusp.publisher.cityNEW YORK
hcfmusp.publisher.countryUSA
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Arquivos
Coleções
Comunicações em Eventos - FM/MCM
Comunicações em Eventos - FM/MPT
Comunicações em Eventos - HC/ICHC
Comunicações em Eventos - LIM/17