Experimental Method for Recording Epicardium Potentials and Cardiac Myocyte Shortening
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Citações na Scopus
0
Tipo de produção
conferenceObject
Data de publicação
2017
Título da Revista
ISSN da Revista
Título do Volume
Editora
IEEE COMPUTER SOC
Autores
MARCHINI, Gustavo S.
TAMASHIRO, Daniel S. U.
Citação
2017 COMPUTING IN CARDIOLOGY (CINC), 2017
Resumo
The hemodynamic changes observed in advanced stages of heart diseases are often accompanied by changes in the electrical and mechanical properties of cardiac myocytes. The objective of this work is to develop an experimental method for recording ventricle epicardium potentials in isolated rat hearts and isolated cardiac myocyte shortening. Briefly, rat heart was removed, aorta was cannulated and coronary arteries were retrogradely perfused with heated and oxygenated buffer solutions. Ag-AgCl electrodes fixed in a silicone pouch placed around the heart were used to measure epicardium potentials. The perfusion was switched to an enzyme-containing solution for digestion of the heart and obtaining isolated cardiac myocytes. Measurements of shortening were made in cells electrically stimulated. The results suggest the possibility of relating the electrical behavior of the whole heart with mechanical properties of cardiac myocytes and may represent an useful tool in basic cardiac research.
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Referências
- BRADY AJ, 1991, PHYSIOL REV, V71, P413
- Chung CS, 2013, PHYSL REPORTS, V1
- Dhein S, 2005, PRACTICAL METHODS CA
- GEDDES LA, 1969, MED BIOL ENG, V7, P49, DOI 10.1007/BF02474669
- Louch WE, 2011, J MOL CELL CARDIOL, V51, P288, DOI 10.1016/j.yjmcc.2011.06.012
- Lu WJ, 2010, COMPUT BIOL MED, V40, P456, DOI 10.1016/j.compbiomed.2010.02.010
- Macchi E, 1998, AM J PHYSIOL-HEART C, V275, pH1886, DOI 10.1152/ajpheart.1998.275.5.H1886
- Pfeiffer ER, 2014, J BIOMECHANICAL ENG, V136
- Xu LZ, 2014, NAT COMMUN, V5, DOI 10.1038/ncomms4329