Periodontopathogenic bacteria in subglottic samples from patients undergoing elective intubation for general anesthesia: A pilot study

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art_MORILLO_Periodontopathogenic_bacteria_in_subglottic_samples_from_patients_undergoing_2021.PDF (662.99 KB)
Citações na Scopus
4
Tipo de produção
article
Data de publicação
2021
DOI
Scopus
Web of Science
PubMed
Título da Revista
ISSN da Revista
Título do Volume
Editora
WILEY
Autores
MORILLO, Carlos M. R.
SARAIVA, Luciana
ROMITO, Giuseppe A.
PANNUTI, Claudio Mendes
PERES, Maria Paula S. M.
VILLAR, Cristina C.
Citação
JOURNAL OF PERIODONTOLOGY, v.92, n.8, p.E94-E102, 2021
Projetos de Pesquisa
Unidades Organizacionais
Fascículo
Resumo
Background Translocation of periodontal pathogens into the respiratory tract could either cause pneumonia or disrupt local defense mechanisms, predisposing the host to infection by respiratory pathogens. The objective of this pilot study was to evaluate the levels of periodontopathogenic bacteria in subglottic samples of intubated and mechanically ventilated patients and the impact of oral decontamination with chlorhexidine (CHX) on subglottic levels of these microorganisms. Methods Patients scheduled to undergo elective surgical procedures requiring endotracheal intubation and mechanical ventilation for at least 3 hours were included. Following full-mouth periodontal examination, patients were randomly assigned to groups that rinsed preoperatively with 0.12% CHX or 0.9% saline (control). After 3 hours of orotracheal intubation, subglottic contents were collected. Quantification of Aggregatibacter actinomycetemcomitans (A. actinomycetemcomitans), Porphyromonas gingivalis (P. gingivalis), and Tannerella forsythia (T. forsythia) in subglottic samples was determined using quantitative real-time polymerase chain reaction. Data were analyzed by Fisher Exact Probability, unpaired Student's t and Mann-Whitney tests. Results Of the 69 patients included, 43 completed study participation. There were no differences between control and CHX groups in subglottic detection rates and abundance levels of P. gingivalis (P = 0.59), T. forsythia (P = 0.83) and A. actinomycetemcomitans (P = 0.07). Moreover, our data indicate that periodontal health has no impact on subglottic levels of P. gingivalis, T. forsythia, and A. actinomycetemcomitans. Conclusions Periodontal pathogens were detected in subglottic samples of intubated and mechanically ventilated patients. Moreover, a single CHX rinse prior to endotracheal intubation may have no effect on subglottic contamination by P. gingivalis, T. forsythia, and A. actinomycetemcomitans.
Palavras-chave
chlorhexidine, healthcare‐, associated pneumonia, microbiology, periodontitis
URI
https://observatorio.fm.usp.br/handle/OPI/42013
Referências
  1. Aas JA, 2005, J CLIN MICROBIOL, V43, P5721, DOI 10.1128/JCM.43.11.5721-5732.2005
  2. AINAMO J, 1975, INT DENT J, V25, P229
  3. [Anonymous], 2005, AM J RESP CRIT CARE, V171, P388, DOI 10.1164/rccm.200405-644ST
  4. Cabov T, 2010, WIEN KLIN WOCHENSCHR, V122, P397, DOI 10.1007/s00508-010-1397-y
  5. Chang JS, 2016, PANCREAS, V45, P134, DOI 10.1097/MPA.0000000000000419
  6. Baptista IMD, 2018, ARCH ORAL BIOL, V85, P64, DOI 10.1016/j.archoralbio.2017.09.029
  7. De Marco Andrea Carvalho, 2013, Rev. odontol. UNESP, V42, P182
  8. Dominy SS, 2019, SCI ADV, V5, DOI 10.1126/sciadv.aau3333
  9. El Attar M. M., 2010, Eastern Mediterranean Health Journal, V16, P563
  10. El-Solh AA, 2004, CHEST, V126, P1575, DOI 10.1378/chest.126.5.1575
  11. Grap MJ, 2004, HEART LUNG, V33, P83, DOI 10.1016/j.hrtlng.2003.12.004
  12. Henderson B, 2010, PERIODONTOL 2000, V54, P78, DOI 10.1111/j.1600-0757.2009.00331.x
  13. INGLIS TJJ, 1995, J HOSP INFECT, V30, P409, DOI 10.1016/0195-6701(95)90045-4
  14. Kinane DF, 2017, NAT REV DIS PRIMERS, V3, DOI 10.1038/nrdp.2017.38
  15. Kollef MH, 2005, CHEST, V128, P3854, DOI 10.1378/chest.128.6.3854
  16. LaMonte MJ, 2017, J AM HEART ASSOC, V6, DOI 10.1161/JAHA.116.004518
  17. Manau C, 2008, J CLIN PERIODONTOL, V35, P385, DOI 10.1111/j.1600-051X.2008.01222.x
  18. Munro CL, 2009, AM J CRIT CARE, V18, P428, DOI 10.4037/ajcc2009792
  19. Nagaoka K, 2017, J INFECT CHEMOTHER, V23, P69, DOI 10.1016/j.jiac.2016.09.013
  20. Needleman IG, 2011, J CLIN PERIODONTOL, V38, P246, DOI 10.1111/j.1600-051X.2010.01688.x
  21. NEWMAN MG, 1976, J PERIODONTOL, V47, P373, DOI 10.1902/jop.1976.47.7.373
  22. OLEARY TJ, 1972, J PERIODONTOL, V43, P38, DOI 10.1902/jop.1972.43.1.38
  23. Ozcaka O, 2012, J PERIODONTAL RES, V47, P584, DOI 10.1111/j.1600-0765.2012.01470.x
  24. Page RC, 2007, J PERIODONTOL, V78, P1387, DOI 10.1902/jop.2007.060264
  25. Pataro AL, 2016, J APPL ORAL SCI, V24, P229, DOI 10.1590/1678-775720150534
  26. Pizzo G, 2010, EUR J INTERN MED, V21, P496, DOI 10.1016/j.ejim.2010.07.011
  27. Ramseier CA, 2009, J PERIODONTOL, V80, P436, DOI [10.1902/jop.2009.080480, 10.1902/jop.2009.080480 ]
  28. Rudney JD, 2003, J PERIODONTAL RES, V38, P465, DOI 10.1034/j.1600-0765.2003.00670.x
  29. Sharma A, 2010, PERIODONTOL 2000, V54, P106, DOI 10.1111/j.1600-0757.2009.00332.x
  30. Shelburne CE, 2000, J MICROBIOL METH, V39, P97, DOI 10.1016/S0167-7012(99)00106-2
  31. Shen TC, 2017, J PERIODONTOL, V88, P723, DOI 10.1902/jop.2017.160414
  32. Socransky SS, 1998, J CLIN PERIODONTOL, V25, P134, DOI 10.1111/j.1600-051X.1998.tb02419.x
  33. Tomita S, 2013, MICROB PATHOGENESIS, V61-62, P11, DOI 10.1016/j.micpath.2013.04.006
  34. Torres A, 2017, EUR RESPIR J, V50, DOI 10.1183/13993003.00582-2017
  35. Villar CC, 2016, RESP CARE, V61, P1245, DOI 10.4187/respcare.04610
  36. Yao LY, 2011, J NURS RES, V19, P289, DOI 10.1097/JNR.0b013e318236d05f
  37. Zenobia C, 2015, VIRULENCE, V6, P236, DOI 10.1080/21505594.2014.999567

Coleções
Artigos e Materiais de Revistas Científicas - FM/MCG
Artigos e Materiais de Revistas Científicas - HC/ICHC
Artigos e Materiais de Revistas Científicas - HC/InCor
Artigos e Materiais de Revistas Científicas - LIM/08
Artigos e Materiais de Revistas Científicas - ODS/03