Indirect Evidence of Circulating Parasite Hapten Immune Complexes in Visceral Leishmaniasis
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Citações na Scopus
3
Tipo de produção
article
Data de publicação
2019
Título da Revista
ISSN da Revista
Título do Volume
Editora
AMER ASSOC CLINICAL CHEMISTRY
Autores
HIRAMOTO, Roberto Mitsuyoshi
PARTATA, Anette Kelsei
Citação
JOURNAL OF APPLIED LABORATORY MEDICINE, v.4, n.3, p.404-409, 2019
Resumo
Background: Hypergammaglobulinemia is present in visceral leishmaniasis (VL), inducing the formation of immune complexes (ICs), which interferes in conventional serology. Parasitic haptens block antibodies, making it difficult to identify and detect them. ICs could be determined indirectly by acid dissociative ELISA (DE) seroconversion in natural and experimental VL. Methods: We determined the frequency of samples that seroconverted in DE or presented a 10% increase in DE (Delta DE) in 3 types of VLs-hamster, canine, and human samples-with larger antigen determination by direct antigen capture in experimental samples. Results: The Delta DE frequency is increased in all VL models: human (34%), canine (27%), and hamster (25%) samples. Seroconversion was present in hamster (14%), dog (1%), and human (6%) samples. During experimental infection, higher frequencies (28%) of circulating antigens were observed at the 30th day, associated with higher Delta DE (47%) and seroconversion (22%), with lower frequencies in other periods. Conclusions: The frequency of Delta ED and seroconversion samples found in natural and experimental infection suggests that specific antibodies can be blocked by low molecular weight antigens that interfere qualitatively (seroconversion) or quantitatively (Delta DE) in serology. Several antigen types may be involved, as high molecular weight proteins and low molecular weight glycoconjugates. The higher frequency of those indirect demonstrations of antibody-blocking antigen or haptens that could be acid-removed in VL has implications for the development of assays for detection of circulating or antibody-bound 1- to 3-kDa antigens, which could interfere in diagnosis and also in the immune response of the host.
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Referências
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