In situ study of cellular immune response in human cutaneous lesions caused by Leishmania (Viannia) panamensis in Panama

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art_GONZALEZ_In_situ_study_of_cellular_immune_response_in_2021.PDF (1.08 MB)
Citações na Scopus
3
Tipo de produção
article
Data de publicação
2021
DOI
Scopus
Web of Science
PubMed
Título da Revista
ISSN da Revista
Título do Volume
Editora
WILEY
Autores
CALZADA, Jose Eduardo
SALDANA, Azael
Citação
PARASITE IMMUNOLOGY, v.43, n.3, article ID e12801, 9p, 2021
Projetos de Pesquisa
Unidades Organizacionais
Fascículo
Resumo
Aims: Leishmaniasis is considered a disease with multiple clinical/immunopathological characteristics, depending on the immunity of the host and the species of the parasite. In Panama, the most prevalent species that causes localized cutaneous leishmaniasis (LCL) is Leishmania (Viannia) panamensis, and its immune response is poorly studied. Therefore, we evaluated by immunohistochemistry, the in situ immune response during this infection. Methods and Results: Biopsies from Panamanian patients with LCL were collected and processed by histological techniques. Infection by L. (V.) panamensis was demonstrated by isolation in culture and molecular characterization by Hsp70-RFLP. The in situ immune response was assessed by immunohistochemistry. The immune response was characterized by predominance of T cells, mainly CD8 cells that showed positive correlation with IFN-gamma and Granzyme B. CD4 cells presented positive correlation with both IFN-gamma and IL-13, pointed by mixed cellular immune response. Regulatory response was characterized by FoxP3 cells, which showed positive correlation to IL-10 but not with TGF-beta. Conclusions: L. (V.) panamensis infection triggers a mixed cellular immune response, characterized by the presence of pro-inflammatory, anti-inflammatory and regulatory elements in the skin lesion of Panamanian patients. These data contribute to a better understanding of the immunopathogenesis of Leishmania Viannia infection in Panama.
Palavras-chave
cutaneous leishmaniasis, cytokines, immune response, immunohistochemistry, Leishmania panamensis, Panama
URI
https://observatorio.fm.usp.br/handle/OPI/39980
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Coleções
Artigos e Materiais de Revistas Científicas - FM/MPT
Artigos e Materiais de Revistas Científicas - LIM/50