Chemical Composition and in vitro Cytotoxic and Antileishmanial Activities of Extract and Essential Oil from Leaves of Piper cernuum
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Citações na Scopus
24
Tipo de produção
article
Data de publicação
2015
Título da Revista
ISSN da Revista
Título do Volume
Editora
NATURAL PRODUCTS INC
Autores
CAPELLO, Tabata M.
MARTINS, Euder G. A.
FARIAS, Camyla F. de
FIGUEIREDO, Carlos R.
MATSUO, Alisson L.
OLIVEIRA-SILVA, Diogo
SARTORELLI, Patricia
LAGO, Joao Henrique G.
Citação
NATURAL PRODUCT COMMUNICATIONS, v.10, n.2, p.285-288, 2015
Resumo
Fractionation of the MeOH extract from leaves of Piper cernuum Veil. (Piperaceae) afforded six phenylpropanoid derivatives: 3',4'-dimethoxydihydrocinnamic acid (1), piplaroxide (2), methyl 4'-hydroxy-3',5'-dimethoxy cinnamate (3), 3',4',5'-trimethoxydihydrocinnamic acid (3), dihydropiplartine (5), and piplartine (6). The structures of isolated metabolites were characterized by NMR and MS spectral data analysis. The chemical composition of essential oil from the leaves was determined using GC/LREIMS followed by the determination of Kovats indexes. This procedure allowed the identification of nineteen terpenoids, with beta-elemene (7), bicyclogermacrene (8), germacrene D (9), and (E)-caryophyllene (10) as the main compounds. Compounds 1 and 3-6 displayed no in vitro cytotoxicity against cancer cell lineages B16F10-Nex2, U87, HeLa, HL-60, HCT, and A2058 while 2 showed moderate activity against B16F10-Nex2 and HL-60 lines. Otherwise, compounds 7-10 displayed high cytotoxic activity. Evaluation against non-tumorigenic HFF cells indicated a reduced selectivity of compounds 7-10 to tumoral cells. No antileishmanial activity on macrophages infected with L. (L.) amazonensis was found for the crude MeOH extract and compounds 1-6. The crude essential oil and compounds 7-10 reduced parasitism and eliminated the majority of infected and non-infected cells at 50 mu g/mL.
Palavras-chave
Piper cernuum Vell., Phenylpropanoid derivatives, Essential oil composition, Cytotoxic, Antiparasitic
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