Multiple inert gas elimination technique by micropore membrane inlet mass spectrometry-a comparison with reference gas chromatography

Imagem de Miniatura
Arquivos
art_BORGES_Multiple_inert_gas_elimination_technique_by_micropore_membrane_2013.PDF (1.7 MB)
Citações na Scopus
16
Tipo de produção
article
Data de publicação
2013
DOI
Scopus
Web of Science
PubMed
Título da Revista
ISSN da Revista
Título do Volume
Editora
AMER PHYSIOLOGICAL SOC
Autores
KRETZSCHMAR, Moritz
SCHILLING, Thomas
VOGT, Andreas
ROTHEN, Hans Ulrich
HACHENBERG, Thomas
LARSSON, Anders
BAUMGARDNER, James E.
HEDENSTIERNA, Goran
Citação
JOURNAL OF APPLIED PHYSIOLOGY, v.115, n.8, p.1107-1118, 2013
Projetos de Pesquisa
Unidades Organizacionais
Fascículo
Resumo
The mismatching of alveolar ventilation and perfusion (V-A/Q) is the major determinant of impaired gas exchange. The gold standard for measuring V-A/Q distributions is based on measurements of the elimination and retention of infused inert gases. Conventional multiple inert gas elimination technique (MIGET) uses gas chromatography (GC) to measure the inert gas partial pressures, which requires tonometry of blood samples with a gas that can then be injected into the chromatograph. The method is laborious and requires meticulous care. A new technique based on micropore membrane inlet mass spectrometry (MMIMS) facilitates the handling of blood and gas samples and provides nearly real-time analysis. In this study we compared MIGET by GC and MMIMS in 10 piglets: 1) 3 with healthy lungs; 2) 4 with oleic acid injury; and 3) 3 with isolated left lower lobe ventilation. The different protocols ensured a large range of normal and abnormal V-A/Q distributions. Eight inert gases (SF6, krypton, ethane, cyclopropane, desflurane, enflurane, diethyl ether, and acetone) were infused; six of these gases were measured with MMIMS, and six were measured with GC. We found close agreement of retention and excretion of the gases and the constructed V-A/Q distributions between GC and MMIMS, and predicted Pa-O2 from both methods compared well with measured Pa-O2. V-A/Q by GC produced more widely dispersed modes than MMIMS, explained in part by differences in the algorithms used to calculate V-A/Q distributions. In conclusion, MMIMS enables faster measurement of V-A/Q, is less demanding than GC, and produces comparable results.
Palavras-chave
MIGET, ventilation/perfusion distributions, inert gases, mass spectrometry, membrane inlet, gas chromatography
URI
https://observatorio.fm.usp.br/handle/OPI/4580
Referências
  1. AGUSTI AGN, 1994, THORAX, V49, P924, DOI 10.1136/thx.49.9.924
  2. ANDERSEN OS, 1960, SCAND J CLIN LAB INV, V12, P172, DOI 10.3109/00365516009062419
  3. Baumgardner JE, 2000, J APPL PHYSIOL, V89, P1699
  4. BAUMGARDNER JE, 1995, J MASS SPECTROM, V30, P563, DOI 10.1002/jms.1190300407
  5. Bland JM, 1999, STAT METHODS MED RES, V8, P135, DOI 10.1191/096228099673819272
  6. Conover WJ, 1999, PRACTICAL NONPARAMET, P456
  7. Dempsey JA, 1999, J APPL PHYSIOL, V87, P1997
  8. Duenges B, 2009, ANESTH ANALG, V109, P1831, DOI 10.1213/ANE.0b013e3181bbc401
  9. EVANS JW, 1977, J APPL PHYSIOL, V42, P889
  10. GALE GE, 1985, J APPL PHYSIOL, V58, P978
  11. GEA J, 1991, ANESTHESIOLOGY, V75, P782, DOI 10.1097/00000542-199111000-00009
  12. HACHENBERG T, 1994, ANESTHESIOLOGY, V80, P509, DOI 10.1097/00000542-199403000-00006
  13. HARDEWIG A, 1960, J APPL PHYSIOL, V15, P723
  14. HEDENSTIERNA G, 1995, THORAX, V50, P85, DOI 10.1136/thx.50.1.85
  15. HLASTALA MP, 1978, J APPL PHYSIOL, V44, P258
  16. KELMAN GR, 1967, RESP PHYSIOL, V3, P111, DOI 10.1016/0034-5687(67)90028-X
  17. KELMAN GR, 1966, J APPL PHYSIOL, V21, P1375
  18. MANIER G, 1994, THORAX, V49, P1169, DOI 10.1136/thx.49.11.1169
  19. MELOT C, 1994, THORAX, V49, P1251, DOI 10.1136/thx.49.12.1251
  20. PETRINI MF, 1983, RESP PHYSIOL, V54, P121, DOI 10.1016/0034-5687(83)90118-4
  21. ROCA J, 1994, THORAX, V49, P815, DOI 10.1136/thx.49.8.815
  22. RODRIGUEZROISIN R, 1990, EUR RESPIR J, V3, P469
  23. RODRIGUEZROISIN R, 1994, THORAX, V49, P1027, DOI 10.1136/thx.49.10.1027
  24. ROTHEN HU, 1995, ANESTHESIOLOGY, V82, P832, DOI 10.1097/00000542-199504000-00004
  25. Serianni R, 2003, J APPL PHYSIOL, V94, P561, DOI 10.1152/japplphysiol.00710.2002
  26. Shimazu T, 1996, J APPL PHYSIOL, V81, P2250
  27. SIGGAARDANDERSEN O, 1995, ACTA ANAESTH SCAND, V39, P41
  28. WAGNER PD, 1974, J APPL PHYSIOL, V36, P600
  29. WAGNER PD, 1974, J CLIN INVEST, V54, P54, DOI 10.1172/JCI107750
  30. Wagner PD, 2008, INTENS CARE MED, V34, P994, DOI 10.1007/s00134-008-1108-6
  31. WAGNER PD, 1977, J CLIN INVEST, V59, P203, DOI 10.1172/JCI108630
  32. West JB, 1977, BIOENGINEERING ASPEC, P361
  33. Zhou JX, 2002, AM J VET RES, V63, P74, DOI 10.2460/AJVR.2002.63.74

Coleções
Artigos e Materiais de Revistas Científicas - LIM/09