The role of interleukin-6, endothelins, and apoptotic genes in small bowel transplantation, in a swine model of ischemia and reperfusion injury

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dc.contributorSistema FMUSP-HC: Faculdade de Medicina da Universidade de São Paulo (FMUSP) e Hospital das Clínicas da FMUSP
dc.contributor.authorPINHO-APEZZATO, Maria Lucia de
dc.contributor.authorMAKSOUD-FILHO, Joao Gilberto
dc.contributor.authorVALINETTI, Emilia Aparecida
dc.contributor.authorSANTOS, Maria Merces
dc.contributor.authorTANNURI, Ana Cristina Aoun
dc.contributor.authorMELLO, Evandro Sobroza
dc.contributor.authorSILVA, Luiz Fernando
dc.contributor.authorCOELHO, Maria Cecilia de Mendonca
dc.contributor.authorGIBELLI, Nelson Elias Mendes
dc.contributor.authorROCHA, Rafael Malagoli
dc.contributor.authorNONOGAKI, Suely
dc.contributor.authorGUIMARAES, Raimundo Renato Nunes
dc.contributor.authorTANNURI, Uenis
dc.date.accessioned2017-11-27T16:33:31Z
dc.date.available2017-11-27T16:33:31Z
dc.date.issued2011
dc.description.abstractIRI is closely related to sepsis in ITx setting. Complete understanding of the mechanisms involved in IRI development may improve outcomes. Ortothopic ITx without immunosuppression was performed in order to characterize IRI-associated mucosal damage. Twenty pigs underwent ITx. Two groups were assigned to different CI times: G1: 90 min and, G2: 180 min. Euro-Collins was used as preservation solution. Jejunal fragments were collected at donor laparotomy, 30 min, and 3 days after reperfusion. IRI assessment involved: histopathologic analysis, quantification of MPO-positive cells through immunohistochemical studies, quantification of epithelial apoptotic cells using TUNEL staining, and quantification of IL-6, ET-1, Bak, and Bcl-XL genes expression by RT-PCR. Neutrophilic infiltration increased in a similar fashion in both groups, but lasted longer in G2. Apoptosis detected by TUNEL staining increased and anti-apoptotic gene Bcl-XL expression decreased significantly in G1, 3 days after surgery. Endothelin-1 and IL-6 genes expression increased 30 min after the procedure and returned to baseline 3 days after surgery. In conclusion, IL-6 and ET-1 are involved precociously in the development of intestinal IRI. Apoptosis was more frequently detected in G1 grafts by TUNEL-staining and by RT-PCR.
dc.description.indexMEDLINE
dc.identifier.citationPEDIATRIC TRANSPLANTATION, v.15, n.6, p.617-627, 2011
dc.identifier.doi10.1111/j.1399-3046.2011.01538.x
dc.identifier.issn1397-3142
dc.identifier.urihttps://observatorio.fm.usp.br/handle/OPI/23304
dc.language.isoeng
dc.publisherWILEY-BLACKWELL
dc.relation.ispartofPediatric Transplantation
dc.rightsrestrictedAccess
dc.rights.holderCopyright WILEY-BLACKWELL
dc.subjectintestinal transplantation
dc.subjectsolid organ transplantation
dc.subjectendothelin
dc.subjectanimal
dc.subject.otherintestinal transplantation
dc.subject.otherischemia/reperfusion injury
dc.subject.otherthrombin inhibition
dc.subject.otheradenosine receptors
dc.subject.othercalcineurin
dc.subject.otherexpression
dc.subject.otherprotection
dc.subject.othermyocardium
dc.subject.otherallograft
dc.subject.wosPediatrics
dc.subject.wosTransplantation
dc.titleThe role of interleukin-6, endothelins, and apoptotic genes in small bowel transplantation, in a swine model of ischemia and reperfusion injury
dc.typearticle
dc.type.categoryoriginal article
dc.type.versionpublishedVersion
dspace.entity.typePublication
hcfmusp.author.externalROCHA, Rafael Malagoli:Univ Sao Paulo, Sch Med, Canc Hosp AC Camargo, Dept Anat Patol, Sao Paulo, Brazil
hcfmusp.author.externalNONOGAKI, Suely:Univ Sao Paulo, Sch Med, Adolfo Lutz Inst, Pathol Ctr, Sao Paulo, Brazil
hcfmusp.citation.scopus10
hcfmusp.contributor.author-fmusphcMARIA LUCIA DE PINHO APEZZATO
hcfmusp.contributor.author-fmusphcJOAO GILBERTO MAKSOUD FILHO
hcfmusp.contributor.author-fmusphcEMILIA APARECIDA VALINETTI
hcfmusp.contributor.author-fmusphcMARIA MERCES SANTOS
hcfmusp.contributor.author-fmusphcANA CRISTINA AOUN TANNURI
hcfmusp.contributor.author-fmusphcEVANDRO SOBROZA DE MELLO
hcfmusp.contributor.author-fmusphcLUIZ FERNANDO FERRAZ DA SILVA
hcfmusp.contributor.author-fmusphcMARIA CECILIA DE MENDONCA COELHO
hcfmusp.contributor.author-fmusphcNELSON ELIAS MENDES GIBELLI
hcfmusp.contributor.author-fmusphcRAIMUNDO RENATO NUNES GUIMARAES
hcfmusp.contributor.author-fmusphcUENIS TANNURI
hcfmusp.description.beginpage617
hcfmusp.description.endpage627
hcfmusp.description.issue6
hcfmusp.description.volume15
hcfmusp.origemWOS
hcfmusp.origem.pubmed21884347
hcfmusp.origem.scopus2-s2.0-80052101043
hcfmusp.origem.wosWOS:000294170200018
hcfmusp.publisher.cityMALDEN
hcfmusp.publisher.countryUSA
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