Generation of Cytotoxic T Cells and Dysfunctional CD8 T Cells in Severe COVID-19 Patients
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Citações na Scopus
8
Tipo de produção
article
Data de publicação
2022
Título da Revista
ISSN da Revista
Título do Volume
Editora
MDPI
Autores
Citação
CELLS, v.11, n.21, article ID 3359, 18p, 2022
Resumo
COVID-19, the infectious disease caused by SARS-CoV-2, has spread on a pandemic scale. The viral infection can evolve asymptomatically or can generate severe symptoms, influenced by the presence of comorbidities. Lymphopenia based on the severity of symptoms in patients affected with COVID-19 is frequent. However, the profiles of CD4+ and CD8+ T cells regarding cytotoxicity and antiviral factor expression have not yet been completely elucidated in acute SARS-CoV-2 infections. The purpose of this study was to evaluate the phenotypic and functional profile of T lymphocytes in patients with moderate and severe/critical COVID-19. During the pandemic period, we analyzed a cohort of 62 confirmed patients with SARS-CoV-2 (22 moderate cases and 40 severe/critical cases). Notwithstanding lymphopenia, we observed an increase in the expression of CD28, a co-stimulator molecule, and activation markers (CD38 and HLA-DR) in T lymphocytes as well as an increase in the frequency of CD4+ T cells, CD8+ T cells, and NK cells that express the immunological checkpoint protein PD-1 in patients with a severe/critical condition compared to healthy controls. Regarding the cytotoxic profile of peripheral blood mononuclear cells, an increase in the response of CD4+ T cells was already observed at the baseline level and scarcely changed upon PMA and Ionomycin stimulation. Meanwhile, CD8+ T lymphocytes decreased the cytotoxic response, evidencing a profile of exhaustion in patients with severe COVID-19. As observed by t-SNE, there were CD4+ T-cytotoxic and CD8+ T with low granzyme production, evidencing their dysfunction in severe/critical conditions. In addition, purified CD8+ T lymphocytes from patients with severe COVID-19 showed increased constitutive expression of differentially expressed genes associated with the caspase pathway, inflammasome, and antiviral factors, and, curiously, had reduced expression of TNF-alpha. The cytotoxic profile of CD4+ T cells may compensate for the dysfunction/exhaustion of TCD8+ in acute SARS-CoV-2 infection. These findings may provide an understanding of the interplay of cytotoxicity between CD4+ T cells and CD8+ T cells in the severity of acute COVID-19 infection.
Palavras-chave
SARS-CoV-2, COVID-19, T-lymphocytes, antiviral response, cytotoxic factors
Referências
- Abdelmoaty MM, 2021, FRONT IMMUNOL, V12, DOI 10.3389/fimmu.2021.741502
- Ahmadi P, 2020, CELLS-BASEL, V9, DOI 10.3390/cells9081750
- Ahmed F, 2020, FRONT IMMUNOL, V11, DOI 10.3389/fimmu.2020.586765
- Andre S, 2022, CELL DEATH DIFFER, V29, P1486, DOI 10.1038/s41418-022-00936-x
- Bacher P, 2020, IMMUNITY, V53, P1258, DOI 10.1016/j.immuni.2020.11.016
- Bader SM, 2022, BIOCHEM J, V479, P609, DOI 10.1042/BCJ20210602
- Bertrand F, 2016, ONCOIMMUNOLOGY, V5, DOI 10.1080/2162402X.2015.1068495
- Bezemer GFG, 2021, FRONT PHARMACOL, V11, DOI 10.3389/fphar.2020.601685
- Bizzotto J, 2020, ISCIENCE, V23, DOI 10.1016/j.isci.2020.101585
- Cao XT, 2020, NAT REV IMMUNOL, V20, P269, DOI 10.1038/s41577-020-0308-3
- Cardinez C, 2018, J EXP MED, V215, P2715, DOI 10.1084/jem.20180639
- Ferlazzo G, 2002, EUR J IMMUNOL, V32, P3092, DOI 10.1002/1521-4141(200211)32:11<3092::AID-IMMU3092>3.0.CO;2-0
- Gandhi RT, 2020, NEW ENGL J MED, V383, P1757, DOI 10.1056/NEJMcp2009249
- Haller O, 2015, TRENDS MICROBIOL, V23, P154, DOI 10.1016/j.tim.2014.12.003
- Herman-Edelstein M, 2021, CARDIOVASC DIABETOL, V20, DOI 10.1186/s12933-021-01275-w
- Huang CL, 2020, LANCET, V395, P497, DOI 10.1016/S0140-6736(20)30183-5
- Huang YZ, 2022, EUR REV MED PHARMACO, V26, P1770, DOI 10.26355/eurrev_202203_28248
- Jafarzadeh A, 2021, SCAND J IMMUNOL, V93, DOI 10.1111/sji.12967
- Kindler E, 2016, ADV VIRUS RES, V96, P219, DOI 10.1016/bs.aivir.2016.08.006
- Koul A, 2020, BIOPHYS J, V118, P2726, DOI 10.1016/j.bpj.2020.04.025
- Lei XB, 2020, NAT COMMUN, V11, DOI 10.1038/s41467-020-17665-9
- Li TS, 2004, J INFECT DIS, V189, P648, DOI 10.1086/381535
- Lin L, 2020, EMERG MICROBES INFEC, V9, P727, DOI 10.1080/22221751.2020.1746199
- Lopez L, 2020, VIRUSES-BASEL, V12, DOI 10.3390/v12121433
- Mazzoni A, 2020, J CLIN INVEST, V130, P4694, DOI [10.1172/JCI138554, 10.1172/JCI138554.]
- Mitsuyama Y, 2021, J INTENSIVE CARE, V9, DOI 10.1186/s40560-021-00591-3
- Narasaraju T, 2020, FRONT PHARMACOL, V11, DOI 10.3389/fphar.2020.00870
- Osuchowski MF, 2021, LANCET RESP MED, V9, P622, DOI 10.1016/S2213-2600(21)00218-6
- Phetsouphanh C, 2017, FRONT IMMUNOL, V8, DOI 10.3389/fimmu.2017.01729
- Qin C, 2020, CLIN INFECT DIS, V71, P762, DOI 10.1093/cid/ciaa248
- Sapkal G, 2020, INDIAN J MED RES, V151, P444, DOI 10.4103/ijmr.IJMR_2232_20
- Schonrich G, 2019, FRONT CELL INFECT MI, V9, DOI 10.3389/fcimb.2019.00207
- Shoaib N, 2021, PLOS ONE, V16, DOI 10.1371/journal.pone.0255999
- Song JW, 2020, NAT COMMUN, V11, DOI 10.1038/s41467-020-17240-2
- Song PP, 2020, CLIN CHIM ACTA, V509, P280, DOI 10.1016/j.cca.2020.06.017
- Taus E, 2022, FRONT IMMUNOL, V13, DOI 10.3389/fimmu.2022.835830
- Vasileiou S, 2020, HAEMATOLOGICA, V105, P235, DOI 10.3324/haematol.2018.206896
- Wang XL, 2020, CELL MOL IMMUNOL, DOI 10.1038/s41423-020-0424-9
- WHO, 2022, WHO COR DIS COVID 19
- Xia HJ, 2020, CELL REP, V33, DOI 10.1016/j.celrep.2020.108234
- Xiong Y, 2020, EMERG MICROBES INFEC, V9, P761, DOI 10.1080/22221751.2020.1747363
- Yuen CK, 2020, EMERG MICROBES INFEC, V9, P1418, DOI 10.1080/22221751.2020.1780953
- Zenarruzabeitia O, 2021, FRONT IMMUNOL, V12, DOI 10.3389/fimmu.2021.655934
- Zheng MJ, 2020, CELL MOL IMMUNOL, V17, P533, DOI 10.1038/s41423-020-0402-2