Directional Topography Influences Adipose Mesenchymal Stromal Cell Plasticity: Prospects for Tissue Engineering and Fibrosis

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dc.contributorSistema FMUSP-HC: Faculdade de Medicina da Universidade de São Paulo (FMUSP) e Hospital das Clínicas da FMUSP
dc.contributor.authorLIGUORI, Gabriel Romero
dc.contributor.authorZHOU, Qihui
dc.contributor.authorLIGUORI, Tacia Tavares Aquinas
dc.contributor.authorBARROS, Guilherme Garcia
dc.contributor.authorKUHN, Philipp Till
dc.contributor.authorMOREIRA, Luiz Felipe Pinho
dc.contributor.authorRIJN, Patrick van
dc.contributor.authorHARMSEN, Martin C.
dc.date.accessioned2019-06-26T17:28:30Z
dc.date.available2019-06-26T17:28:30Z
dc.date.issued2019
dc.description.abstractIntroduction. Progenitor cells cultured on biomaterials with optimal physical-topographical properties respond with alignment and differentiation. Stromal cells from connective tissue can adversely differentiate to profibrotic myofibroblasts or favorably to smooth muscle cells (SMC). We hypothesized that myogenic differentiation of adipose tissue-derived stromal cells (ASC) depends on gradient directional topographic features. Methods. Polydimethylsiloxane (PDMS) samples with nanometer and micrometer directional topography gradients (wavelength49-3, 425nm) were fabricated. ASC were cultured on patterned PDMS and stimulated with TGF-1 to induce myogenic differentiation. Cellular alignment and adhesion were assessed by immunofluorescence microscopy after 24h. After seven days, myogenic differentiation was examined by immunofluorescence microscopy, gene expression, and immunoblotting. Results. Cell alignment occurred on topographies larger than w=1758nm/a=630nm. The number and total area of focal adhesions per cell were reduced on topographies from w=562nm/a=96nm to w=3919nm/a=1430nm. Focal adhesion alignment was increased on topographies larger than w=731nm/a=146nm. Less myogenic differentiation of ASC occurred on topographies smaller than w=784nm/a=209nm. Conclusion. ASC adherence, alignment, and differentiation are directed by topographical cues. Our evidence highlights a minimal topographic environment required to facilitate the development of aligned and differentiated cell layers from ASC. These data suggest that nanotopography may be a novel tool for inhibiting fibrosis.eng
dc.description.indexPubMedeng
dc.description.sponsorshipUniversity Medical Centre Groningen, University of Groningen, Groningen, the Netherlands
dc.description.sponsorshipNWO-ZonMw Medium Investment Grant [40-00506-98-9021]
dc.description.sponsorshipChina Scholarship Council [201406630003]
dc.identifier.citationSTEM CELLS INTERNATIONAL, article ID 5387850, 14p, 2019
dc.identifier.doi10.1155/2019/5387850
dc.identifier.eissn1687-9678
dc.identifier.issn1687-966X
dc.identifier.urihttps://observatorio.fm.usp.br/handle/OPI/32446
dc.language.isoeng
dc.publisherHINDAWI LTDeng
dc.relation.ispartofStem Cells International
dc.rightsrestrictedAccesseng
dc.rights.holderCopyright HINDAWI LTDeng
dc.subject.wosCell & Tissue Engineeringeng
dc.titleDirectional Topography Influences Adipose Mesenchymal Stromal Cell Plasticity: Prospects for Tissue Engineering and Fibrosiseng
dc.typearticleeng
dc.type.categoryoriginal articleeng
dc.type.versionpublishedVersioneng
dspace.entity.typePublication
hcfmusp.affiliation.countryHolanda
hcfmusp.affiliation.countryChina
hcfmusp.affiliation.countryisocn
hcfmusp.affiliation.countryisonl
hcfmusp.author.externalZHOU, Qihui:Univ Groningen, Univ Med Ctr Groningen, Dept Biomed Engn FB40, WJ Kolff Inst Biomed Engn & Mat Sci FB41, A Deusinglaan 1, NL-9713 AV Groningen, Netherlands; Qingdao Univ, Inst Translat Med, State Key Lab Biofibers & Ecotext, Qingdao 266021, Shandong, Peoples R China
hcfmusp.author.externalLIGUORI, Tacia Tavares Aquinas:Univ Groningen, Univ Med Ctr Groningen, Dept Pathol & Med Biol, Groningen, Netherlands; Univ Sao Paulo, Lab Cirurgia Cardiovasc & Fisiopatol Circulacao L, Inst Coracao InCor, Hosp Clin HCFMUSP,Fac Med, Sao Paulo, SP, Brazil
hcfmusp.author.externalKUHN, Philipp Till:Univ Groningen, Univ Med Ctr Groningen, Dept Biomed Engn FB40, WJ Kolff Inst Biomed Engn & Mat Sci FB41, A Deusinglaan 1, NL-9713 AV Groningen, Netherlands
hcfmusp.author.externalRIJN, Patrick van:Univ Groningen, Univ Med Ctr Groningen, Dept Biomed Engn FB40, WJ Kolff Inst Biomed Engn & Mat Sci FB41, A Deusinglaan 1, NL-9713 AV Groningen, Netherlands
hcfmusp.author.externalHARMSEN, Martin C.:Univ Groningen, Univ Med Ctr Groningen, Dept Pathol & Med Biol, Groningen, Netherlands
hcfmusp.citation.scopus28
hcfmusp.contributor.author-fmusphcGABRIEL ROMERO LIGUORI
hcfmusp.contributor.author-fmusphcGUILHERME GARCIA BARROS
hcfmusp.contributor.author-fmusphcLUIZ FELIPE PINHO MOREIRA
hcfmusp.description.articlenumber5387850
hcfmusp.origemWOS
hcfmusp.origem.pubmed31191675
hcfmusp.origem.scopus2-s2.0-85070672799
hcfmusp.origem.wosWOS:000468529100001
hcfmusp.publisher.cityLONDONeng
hcfmusp.publisher.countryENGLANDeng
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