Dynamics of immunosuppression in hamsters with experimental visceral leishmaniasis

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dc.contributorSistema FMUSP-HC: Faculdade de Medicina da Universidade de São Paulo (FMUSP) e Hospital das Clínicas da FMUSP
dc.contributor.authorFAZZANI, C.
dc.contributor.authorGUEDES, P. A.
dc.contributor.authorSENA, A.
dc.contributor.authorSOUZA, E. B.
dc.contributor.authorGOTO, H.
dc.contributor.authorLINDOSO, J. A. L.
dc.date.accessioned2017-11-27T16:33:57Z
dc.date.available2017-11-27T16:33:57Z
dc.date.issued2011
dc.description.abstractImmunosuppression has been reported to occur during active visceral leishmaniasis and some factors such as the cytokine profile may be involved in this process. In the mouse model of cutaneous leishmaniasis using Leishmania (Leishmania) major, the Th1 response is related to protection while the Th2 response is related to disease progression. However, in hamsters, which are considered to be an excellent model for the study of visceral leishmaniasis, this dichotomy is not observed. Using outbred 45- to 60-day-old (140 to 150 g) male hamsters infected intraperitoneally with 2 x 10(7) L. (L.) chagasi amastigotes, we evaluated the immune response of spleen cells and the production of cytokines. We used 3 to 7 hamsters per group evaluated. We detected a preserved response to concanavalin A measured by index of proliferation during all periods of infection studied, while a proliferative response to Leishmania antigen was detected only at 48 and 72 h post-infection. Messenger RNA from cytokines type 1 (IL-2, TNF-alpha, IFN-gamma) and type 2 (IL-4, IL-10 and TGF-beta) detected by reverse transcriptase polymerase chain reaction and produced by spleen cells showed no qualitative difference between control non-infected hamsters and infected hamsters during any period of infection evaluated. Cytokines were measured by the DNA band intensity on agarose gel using the Image Lab 1D L340 software with no differences observed. In conclusion, the present results showed an antigen-dependent immunosuppression in hamsters with active visceral leishmaniasis that was not related to the cytokine profile.
dc.description.indexMEDLINE
dc.description.sponsorshipCAPES
dc.description.sponsorshipFAPESP
dc.description.sponsorshipCNPq
dc.identifier.citationBRAZILIAN JOURNAL OF MEDICAL AND BIOLOGICAL RESEARCH, v.44, n.7, p.666-670, 2011
dc.identifier.doi10.1590/S0100-879X2011007500062
dc.identifier.issn0100-879X
dc.identifier.urihttps://observatorio.fm.usp.br/handle/OPI/23329
dc.language.isoeng
dc.publisherASSOC BRAS DIVULG CIENTIFICA
dc.relation.ispartofBrazilian Journal of Medical and Biological Research
dc.rightsopenAccess
dc.rights.holderCopyright ASSOC BRAS DIVULG CIENTIFICA
dc.subjectImmunosuppression
dc.subjectLeishmania (Leishmania) chagasi
dc.subjectHamster
dc.subjectCytokine
dc.subjectT-cell proliferation
dc.subject.otherdonovani infected hamsters
dc.subject.othergrowth-factor-beta
dc.subject.othermurine leishmaniasis
dc.subject.othernitric-oxide
dc.subject.otherexpression
dc.subject.otherimmunity
dc.subject.othercells
dc.subject.otherimpairment
dc.subject.otherantigens
dc.subject.otherantibody
dc.subject.wosBiology
dc.subject.wosMedicine, Research & Experimental
dc.titleDynamics of immunosuppression in hamsters with experimental visceral leishmaniasis
dc.typearticle
dc.type.categoryoriginal article
dc.type.versionpublishedVersion
dspace.entity.typePublication
hcfmusp.author.externalGUEDES, P. A.:Univ Sao Paulo, Fac Med, Inst Med Trop Sao Paulo, Lab Soroepidemiol & Imunobiol LIM 38, BR-05403000 Sao Paulo, Brazil
hcfmusp.author.externalSENA, A.:Univ Sao Paulo, Fac Med, Inst Med Trop Sao Paulo, Lab Soroepidemiol & Imunobiol LIM 38, BR-05403000 Sao Paulo, Brazil
hcfmusp.citation.scopus5
hcfmusp.contributor.author-fmusphcCAMILA FAZZANI
hcfmusp.contributor.author-fmusphcEDNA BARBOSA DE SOUZA
hcfmusp.contributor.author-fmusphcHIRO GOTO
hcfmusp.contributor.author-fmusphcJOSE ANGELO LAULETTA LINDOSO
hcfmusp.description.beginpage666
hcfmusp.description.endpage670
hcfmusp.description.issue7
hcfmusp.description.volume44
hcfmusp.origemWOS
hcfmusp.origem.pubmed21584442
hcfmusp.origem.scopus2-s2.0-79961084718
hcfmusp.origem.wosWOS:000293651700009
hcfmusp.publisher.citySAO PAULO
hcfmusp.publisher.countryBRAZIL
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hcfmusp.scopus.lastupdate2024-05-10
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