Evidence for a protective role of Protein Disulfide Isomerase-A1 against aortic dissection
Carregando...
Citações na Scopus
1
Tipo de produção
article
Data de publicação
2023
Título da Revista
ISSN da Revista
Título do Volume
Editora
ELSEVIER IRELAND LTD
Autores
FERNANDES, Carolina Goncalves
SANTOS, Patricia Nolasco
Citação
ATHEROSCLEROSIS, v.382, article ID 117283, 9p, 2023
Resumo
Background and aims: Redox signaling is involved in the pathophysiology of aortic aneurysm/dissection. Protein Disulfide Isomerases and its prototype PDIA1 are thiol redox chaperones mainly from endoplasmic reticulum (ER), while PDIA1 cell surface pool redox-regulates thrombosis, cytoskeleton remodeling and integrin activation, which are mechanisms involved in aortic disease. Here we investigate the roles of PDIA1 in aortic dissection. Methods: Initially, we assessed the outcome of aortic aneurysm/dissection in transgenic PDIA1-overexpressing FVB mice using a model of 28-day exposure to lysyl oxidase inhibitor BAPN plus angiotensin-II infusion. In a second protocol, we assessed the effects of PDIA1 inhibitor isoquercetin (IQ) against aortic dissection in C57BL/6 mice exposed to BAPN for 28 days. Results: Transgenic PDIA1 overexpression associated with ca. 50% (p = 0.022) decrease (vs.wild-type) in mor-tality due to abdominal aortic rupture and protected against elastic fiber breaks in thoracic aorta. Conversely, exposure of mice to IQ increased thoracic aorta dissection-related mortality rates, from ca. 18%-50% within 28-days (p = 0.019); elastic fiber disruption and collagen deposition were also enhanced. The structurally-related compound diosmetin, which does not inhibit PDI, had negligible effects. In parallel, stretch-tension curves indicated that IQ amplified a ductile-type of biomechanical failure vs. control or BAPN-exposed mice aortas. IQ-induced effects seemed unassociated with nonspecific antioxidant effects or ER stress. In both models, echo-cardiographic analysis of surviving mice suggested that aortic rupture was dissociated from progressive dilatation. Conclusions: Our data indicate a protective role of PDIA1 against aortic dissection/rupture and potentially un-covers a novel integrative mechanism coupling redox and biomechanical homeostasis in vascular remodeling.
Palavras-chave
Aortic dissection, Protein disulfide isomerase, Isoquercetin, Aortic aneurysm, Vascular biomechanics
Referências
- Al-abcha A, 2020, AM J CARDIOL, V128, P101, DOI 10.1016/j.amjcard.2020.04.034
- Canelón SP, 2016, PLOS ONE, V11, DOI 10.1371/journal.pone.0166392
- Cao GM, 2022, CELL COMMUN SIGNAL, V20, DOI 10.1186/s12964-022-00993-2
- Chau KH, 2013, PROG CARDIOVASC DIS, V56, P74, DOI 10.1016/j.pcad.2013.05.007
- Clément M, 2019, ARTERIOSCL THROM VAS, V39, P1149, DOI 10.1161/ATVBAHA.118.311727
- de la Fuente-Alonso A, 2021, NAT COMMUN, V12, DOI 10.1038/s41467-021-22933-3
- Dinesh NEH, 2019, HERZ, V44, P138, DOI 10.1007/s00059-019-4786-7
- Fernandes DC, 2021, FREE RADICAL BIO MED, V162, P603, DOI 10.1016/j.freeradbiomed.2020.11.020
- Gaspar RS, 2021, ANTIOXID REDOX SIGN, V35, P1116, DOI 10.1089/ars.2021.0086
- Han L, 2018, AGING-US, V10, P371, DOI 10.18632/aging.101394
- Harky Amer, 2019, Vasc Biol, V1, pR13, DOI 10.1530/VB-19-0027
- Humphrey JD, 2015, CIRC RES, V116, P1448, DOI 10.1161/CIRCRESAHA.114.304936
- Humphrey JD, 2014, SCIENCE, V344, P476, DOI 10.1126/science.1253026
- Iddawela Sashini, 2021, Vasc Biol, V3, pR25, DOI 10.1530/VB-20-0015
- Jasuja R, 2012, J CLIN INVEST, V122, P2104, DOI 10.1172/JCI61228
- Jia LX, 2015, J PATHOL, V236, P373, DOI 10.1002/path.4534
- Jiménez-Altayó F, 2018, FREE RADICAL BIO MED, V118, P44, DOI 10.1016/j.freeradbiomed.2018.02.023
- Kim HW, 2017, AM J PHYSIOL-HEART C, V313, pH1168, DOI 10.1152/ajpheart.00296.2017
- Kim HW, 2016, ARTERIOSCL THROM VAS, V36, P2138, DOI 10.1161/ATVBAHA.116.308356
- Kuzmik GA, 2012, J VASC SURG, V56, P565, DOI 10.1016/j.jvs.2012.04.053
- Laurindo FRM, 2012, FREE RADICAL BIO MED, V52, P1954, DOI 10.1016/j.freeradbiomed.2012.02.037
- Li DY, 2020, J CARDIOVASC PHARM, V75, P229, DOI 10.1097/FJC.0000000000000785
- Lin L, 2015, J BIOL CHEM, V290, P23543, DOI 10.1074/jbc.M115.666180
- McLaughlin SH, 1998, BIOCHEM J, V331, P793, DOI 10.1042/bj3310793
- Mozaffarian D, 2018, CIRC RES, V122, P369, DOI 10.1161/CIRCRESAHA.117.309008
- Navas-Madroñal M, 2019, CLIN SCI, V133, P1421, DOI 10.1042/CS20190399
- Nolasco P, 2020, BBA-MOL BASIS DIS, V1866, DOI 10.1016/j.bbadis.2019.165587
- Oller J, 2021, CIRCULATION, V143, P2091, DOI 10.1161/CIRCULATIONAHA.120.051171
- Oteiza PI, 2018, MOL ASPECTS MED, V61, P41, DOI 10.1016/j.mam.2018.01.001
- Paes AMD, 2011, J LEUKOCYTE BIOL, V90, P799, DOI 10.1189/jlb.0610324
- Parker SJ, 2018, AM J PHYSIOL-HEART C, V315, pH1112, DOI 10.1152/ajpheart.00089.2018
- Perdikaris P, 2014, ANN BIOMED ENG, V42, P1012, DOI 10.1007/s10439-014-0970-3
- Pescatore LA, 2012, J BIOL CHEM, V287, P29290, DOI 10.1074/jbc.M112.394551
- Petsophonsakul P, 2019, ARTERIOSCL THROM VAS, V39, P1351, DOI 10.1161/ATVBAHA.119.312787
- Pinard A, 2019, CIRC RES, V124, P588, DOI 10.1161/CIRCRESAHA.118.312436
- Quintana RA, 2019, CIRC RES, V124, P607, DOI 10.1161/CIRCRESAHA.118.313187
- Ren WH, 2016, SCI REP-UK, V6, DOI 10.1038/srep28149
- Serreli G, 2019, FOOD FUNCT, V10, P6999, DOI [10.1039/c9fo01733j, 10.1039/C9FO01733J]
- Siu KL, 2014, PLOS ONE, V9, DOI 10.1371/journal.pone.0088899
- Moretti AIS, 2017, ARCH BIOCHEM BIOPHYS, V617, P106, DOI 10.1016/j.abb.2016.11.007
- Stepien KL, 2022, INT J MOL SCI, V23, DOI 10.3390/ijms231911078
- Sun WL, 2021, CELL REP, V36, DOI 10.1016/j.celrep.2021.109641
- Tanaka LY, 2020, ANTIOXID REDOX SIGN, V33, P280, DOI 10.1089/ars.2019.8012
- Tanaka LY, 2019, AM J PHYSIOL-HEART C, V316, pH566, DOI 10.1152/ajpheart.00379.2018
- Tanaka LY, 2017, FREE RADICAL BIO MED, V109, P11, DOI 10.1016/j.freeradbiomed.2017.01.025
- Tanaka LY, 2016, HYPERTENSION, V67, P613, DOI 10.1161/HYPERTENSIONAHA.115.06177
- Valentová K, 2014, FOOD CHEM TOXICOL, V68, P267, DOI 10.1016/j.fct.2014.03.018
- Wang KJ, 2020, J CARDIOVASC PHARM, V76, P86, DOI 10.1097/FJC.0000000000000837
- Wang L, 2012, EUR J PHARMACOL, V690, P133, DOI 10.1016/j.ejphar.2012.06.018
- Watanabe K, 2020, J BIOL CHEM, V295, P10092, DOI 10.1074/jbc.RA120.013753
- Winterbourn CC, 2008, FREE RADICAL BIO MED, V45, P549, DOI 10.1016/j.freeradbiomed.2008.05.004
- Yue JN, 2020, ANN VASC SURG, V67, P474, DOI 10.1016/j.avsg.2020.03.002
- Zwicker JI, 2019, JCI INSIGHT, V4, DOI 10.1172/jci.insight.125851