Genetic and virulence characterization of colistin-resistant and colistin-sensitive A. baumannii clinical isolates
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Citações na Scopus
6
Tipo de produção
article
Data de publicação
2019
Título da Revista
ISSN da Revista
Título do Volume
Editora
ELSEVIER SCIENCE INC
Autores
LEITE, Gleice Cristina
STABLER, Richard A.
NEVES, Patricia
Citação
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE, v.95, n.1, p.99-101, 2019
Resumo
Treatment of infections caused by A. baumannii is becoming a challenge due to the ability to develop multidrug-resistance, virulence, and high mortality. We described the colistin resistance and virulence genes present in sixA. baumannii clinical isolates using WGS, expression by qPCR, and virulence in the Galleria mellonella model. The colistin-resistant isolates were assigned as ST233 and the colistin-susceptible isolates as ST236 and ST407. The colistin-resistant isolates contained mutations within PmrA/PmrB, and the pmrA showed up-regulation in all of them. Only one colistin-resistant isolate indicating virulence in G. mellonella. This particular isolate belonged to a different clone, and it was the only isolate that presented non-synonymous mutations in pmrB. Colistinresistance in A. baumannii isolates seems to be caused by up-regulation of pmrA gene. Only one isolate appeared to be virulent in the G. mellonella model. This finding indicating low virulence in isolates belonging to emerging clones circulating in our hospital.
Palavras-chave
Acinetobacter baumannii, Galleria mellonella, whole-sequencing, colistin-resistant, resistance, virulence
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