Profile of differentially expressed Toll-like receptor signaling genes in the natural killer cells of patients with Sezary syndrome
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Citações na Scopus
6
Tipo de produção
article
Data de publicação
2017
Título da Revista
ISSN da Revista
Título do Volume
Editora
IMPACT JOURNALS LLC
Citação
ONCOTARGET, v.8, n.54, p.92183-92194, 2017
Resumo
Sezary syndrome (SS), an aggressive and leukemic form of cutaneous T-cell lymphoma, usually results in shortened survival. Improving innate immunity in SS by targeting natural killer (NK) cells with Toll-like receptor (TLR) agonists could be an interesting modulatory strategy. We evaluated the NK cell populations in SS patients assessing activating and inhibitory receptors expression and profiled the differential expression of TLR signaling pathway genes in unstimulated NK cells and after TLR7/8 stimulation. We observed preserved CD56(bright) NK cells and a low percentage of CD56(dim) NK cells in the peripheral blood of SS patients compared to those in the healthy control group. Both NK cell populations showed down-modulation of NKG2C and NKG2D expression, which was associated with high serum levels of the soluble form of NKG2D ligands. In contrast, an expansion of ""memory"" CD57+ NKG2C+ NK cells and high cytomegalovirus antibody titers were detected in SS patients. Profiling of the TLR signaling genes in NK cells from SS patients showed an abundance of differentially expressed genes (DEGs) in NK cells in the unstimulated condition, with mostly up-regulation of NF kappa B/JNK p38 pathway genes, but there was down-regulation of type I (IFN-alpha/beta) and II (IFN-gamma) interferon and IL-12A. After activation of NK cells with TLR7/8 agonist, the down-regulated genes correlated with the IFN response, and IL-12 became up-regulated, together with other antitumor factors. NK cell activation with a dual agonist for TLR7 and TLR8 is able to induce the expression of IFN-gamma and type I IFN, which can improve immunity in SS patients.
Palavras-chave
Sezary syndrome, natural killer cells, Toll-like receptor 7/8, memory NK cells, differentially expressed genes
Referências
- Bahler DW, 2008, CYTOM PART B-CLIN CY, V74B, P156, DOI 10.1002/cyto.b.20395
- Ballanger F, 2009, EXP DERMATOL, V18, P574, DOI 10.1111/j.1600-0625.2008.00817.x
- Bath-Hextall FJ, 2007, COCHRANE DB SYST REV, DOI 10.1002/14651858.CD003412.pub2
- Bauernhofer T, 2003, EUR J IMMUNOL, V33, P119, DOI 10.1002/immu.200390014
- Beutler B, 2007, IMMUNOL REV, V220, P113, DOI 10.1111/j.1600-065X.2007.00577.x
- Bouaziz JD, 2005, J INVEST DERMATOL, V125, P1273, DOI 10.1111/j.0022-202X.2005.23914.x
- Burns RP, 2000, CLIN IMMUNOL, V94, P13, DOI 10.1006/clim.1999.4804
- Capriotti E, 2008, LEUKEMIA LYMPHOMA, V49, P1190, DOI 10.1080/10428190802064917
- Carrel A, 1910, J AMER MED ASSOC, V55, P1379
- Cerwenka A, 2016, NAT REV IMMUNOL, V16, P112, DOI 10.1038/nri.2015.9
- Cooper MA, 2001, TRENDS IMMUNOL, V22, P633, DOI 10.1016/S1471-4906(01)02060-9
- Della Chiesa M, 2013, IMMUNOL LETT, V155, P11, DOI 10.1016/j.imlet.2013.09.020
- Dendorfer M, 2003, EUR J DERMATOL, V13, P80
- Eisele G, 2006, BRAIN, V129, P2416, DOI 10.1093/brain/awl205
- Ghazi B, 2015, J INVEST DERMATOL, V135, P229, DOI 10.1038/jid.2014.286
- Groh V, 2002, NATURE, V419, P734, DOI 10.1038/nature01112
- Herne KL, 2003, BLOOD, V101, P2132, DOI 10.1182/blood-2002-07-2247
- Horwitz SM, 2008, CLIN LYMPHOMA MYELOM, V8, pS187, DOI 10.3816/CLM.2008.s.015
- Kared H, 2016, CANCER IMMUNOL IMMUN, V65, P441, DOI 10.1007/s00262-016-1803-z
- Kim EJ, 2005, J CLIN INVEST, V115, P798, DOI 10.1172/JCI200524826
- Kopp KLM, 2010, LEUKEMIA, V24, P1179, DOI 10.1038/leu.2010.66
- Korman N, 2005, ARCH DERMATOL, V141, P467, DOI 10.1001/archderm.141.4.467
- Ksienzyk A, 2011, CANCER RES, V71, P6410, DOI 10.1158/0008-5472.CAN-11-1565
- LAROCHE L, 1983, J IMMUNOL, V130, P2467
- Lebwohl M, 2004, J AM ACAD DERMATOL, V50, P714, DOI 10.1016/j.jaad.2003.12.010
- Lee J, 2003, P NATL ACAD SCI USA, V100, P6646, DOI 10.1073/pnas.0631696100
- Lee JC, 2004, J IMMUNOL, V172, P7335
- Livak KJ, 2001, METHODS, V25, P402, DOI 10.1006/meth.2001.1262
- Lopez-Verges S, 2010, BLOOD, V116, P3865, DOI 10.1182/blood-2010-04-282301
- Ma XR, 2013, ONCOIMMUNOLOGY, V2, DOI 10.4161/onci.22647
- Manfrere KCG, 2016, ONCOTARGET, V7, P74592, DOI 10.18632/oncotarget.12816
- Moretta L, 2010, BLOOD, V116, P3689, DOI 10.1182/blood-2010-09-303057
- Nielsen CM, 2015, J IMMUNOL, V194, P4657, DOI 10.4049/jimmunol.1403080
- Olsen E, 2007, BLOOD, V110, P1713, DOI 10.1182/blood-2007-03-055749
- Orange JS, 2013, J ALLERGY CLIN IMMUN, V132, P515, DOI 10.1016/j.jaci.2013.07.020
- Parodi M, 2015, ONCOIMMUNOLOGY, V4, DOI 10.1080/2162402X.2015.1052353
- Peres K, 2003, EUR J DERMATOL, V13, P413
- Peris K, 2003, EUR J DERMATOL, V13, P5
- Rolle A, 2014, J CLIN INVEST, V124, P5305, DOI 10.1172/JCI77440
- ROOK AH, 1995, HEMATOL ONCOL CLIN N, V9, P997
- ROOK AH, 1993, ARCH DERMATOL, V129, P486, DOI 10.1001/archderm.129.4.486
- Rook AH, 2015, BLOOD, V126, P1452, DOI 10.1182/blood-2015-02-630335
- Sanchez-Correa B, 2016, CANCER IMMUNOL IMMUN, V65, P453, DOI 10.1007/s00262-015-1720-6
- Schon MP, 2008, ONCOGENE, V27, P190, DOI 10.1038/sj.onc.1210913
- Schulze HJ, 2005, BRIT J DERMATOL, V152, P939, DOI 10.1111/j.1365-2133.2005.06486.x
- SIDKY YA, 1992, CANCER RES, V52, P3528
- Spaccarelli N, 2015, DERMATOL CLIN, V33, P731, DOI 10.1016/j.det.2015.05.008
- Steinmann A, 2000, J AM ACAD DERMATOL, V43, P555, DOI 10.1067/mjd.2000.107954
- Stockfleth E, 2001, BRIT J DERMATOL, V144, P1050, DOI 10.1046/j.1365-2133.2001.04197.x
- Su MW, 2003, CANCER RES, V63, P7122
- Su SA, 2017, ONCOTARGET, V8, P25700, DOI 10.18632/oncotarget.14878
- Suchin KR, 2002, ARCH DERMATOL, V138, P1137, DOI 10.1001/archderm.138.9.1137
- Tomai MA, 2007, EXPERT REV VACCINES, V6, P835, DOI 10.1586/14760584.6-5.835
- Tracey L, 2003, BLOOD, V102, P1042, DOI 10.1182/blood-2002-11-3574
- Umezu T, 2013, ONCOGENE, V32, P2747, DOI 10.1038/onc.2012.295
- Vasilakos JP, 2013, EXPERT REV VACCINES, V12, P809, DOI 10.1586/14760584.2013.811208
- Vivier E, 2002, CURR OPIN IMMUNOL, V14, P306, DOI 10.1016/S0952-7915(02)00337-0
- VOWELS BR, 1992, J INVEST DERMATOL, V99, P90, DOI 10.1111/1523-1747.ep12611877
- Wagner TL, 1997, CYTOKINE, V9, P837, DOI 10.1006/cyto.1997.0239
- WOOD GS, 1991, AM J PATHOL, V138, P1545
- Wysocka M, 2004, BLOOD, V104, P4142, DOI 10.1182/blood-2004-03-1190
- Wysocka M, 2011, LEUKEMIA LYMPHOMA, V52, P1970, DOI 10.3109/10428194.2011.582202
- Yoo EK, 2001, J AM ACAD DERMATOL, V45, P208, DOI 10.1067/mjd.2001.116345
- Zeitouni NC, 2005, BRIT J DERMATOL, V152, P376, DOI 10.1111/j.1365-2133.2005.06307.x
Coleções
Artigos e Materiais de Revistas Científicas - FM/MPT
Artigos e Materiais de Revistas Científicas - FM/MDT
Artigos e Materiais de Revistas Científicas - HC/ICHC
Artigos e Materiais de Revistas Científicas - LIM/53
Artigos e Materiais de Revistas Científicas - LIM/56
Artigos e Materiais de Revistas Científicas - ODS/03
Artigos e Materiais de Revistas Científicas - FM/MDT
Artigos e Materiais de Revistas Científicas - HC/ICHC
Artigos e Materiais de Revistas Científicas - LIM/53
Artigos e Materiais de Revistas Científicas - LIM/56
Artigos e Materiais de Revistas Científicas - ODS/03