Discriminating Neoplastic and Normal Brain Tissues in Vitro Through Raman Spectroscopy: A Principal Components Analysis Classification Model

Página do item simplificado
dc.contributorSistema FMUSP-HC: Faculdade de Medicina da Universidade de São Paulo (FMUSP) e Hospital das Clínicas da FMUSP
dc.contributor.authorAGUIAR, Ricardo Pinto
dc.contributor.authorSILVEIRA JR., Landulfo
dc.contributor.authorFALCAO, Edgar Teixeira
dc.contributor.authorPACHECO, Marcos Tadeu Tavares
dc.contributor.authorZANGARO, Renato Amaro
dc.contributor.authorPASQUALUCCI, Carlos Augusto
dc.date.accessioned2014-04-25T21:54:32Z
dc.date.available2014-04-25T21:54:32Z
dc.date.issued2013
dc.description.abstractBackground and objective: Because of their aggressiveness, brain tumors can lead to death within a short time after diagnosis. Optical techniques such as Raman spectroscopy may be a technique of choice for in situ tumor diagnosis, with potential use in determining tumor margins during surgery because of its ability to identify biochemical changes between normal and tumor brain tissues quickly and without tissue destruction. Methods: In this work, fragments of brain tumor (glioblastoma, medulloblastoma, and meningioma) and normal tissues (cerebellum and meninges) were obtained from excisional intracranial surgery and from autopsies, respectively. Raman spectra (dispersive spectrometer, 830nm 350mW, 50sec accumulation, total 172 spectra) were obtained in vitro on these fragments. It has been developed as a model to discriminate between the spectra of normal tissue and tumors based on the scores of principal component analysis (PCA) and Euclidean distance. Results: ANOVA indicated that the scores of PC2 and PC3 show differences between normal and tumor groups (p<0.05) which could be employed in a discrimination model. PC2 was able to discriminate glioblastoma from the other tumors and from normal tissues, showing featured peaks of lipids/phospholipids and cholesterol. PC3 discriminated medulloblastoma and meningioma from normal tissues, with the most intense spectral features of proteins. PC3 also discriminated normal tissues (meninges and cerebellum) by the presence of cholesterol peaks. Results indicated a sensitivity and specificity of 97.4% and 100%, respectively, for this in vitro diagnosis of brain tumor. Conclusions: The PCA/Euclidean distance model was effective in differentiating tumor from normal spectra, regardless of the type of tissue (meninges or cerebellum).
dc.description.indexMEDLINE
dc.description.sponsorshipFAPESP (Sao Paulo Research Foundation) [2009/01788-5]
dc.identifier.citationPHOTOMEDICINE AND LASER SURGERY, v.31, n.12, p.595-604, 2013
dc.identifier.doi10.1089/pho.2012.3460
dc.identifier.eissn1557-8550
dc.identifier.issn1549-5418
dc.identifier.urihttps://observatorio.fm.usp.br/handle/OPI/5173
dc.language.isoeng
dc.publisherMARY ANN LIEBERT, INC
dc.relation.ispartofPhotomedicine and Laser Surgery
dc.rightsopenAccess
dc.rights.holderCopyright MARY ANN LIEBERT, INC
dc.subject.othertumors
dc.subject.otheridentification
dc.subject.othercarcinoma
dc.subject.otherdiagnosis
dc.subject.otherstomach
dc.subject.otherlipids
dc.subject.wosSurgery
dc.titleDiscriminating Neoplastic and Normal Brain Tissues in Vitro Through Raman Spectroscopy: A Principal Components Analysis Classification Model
dc.typearticle
dc.type.categoryoriginal article
dc.type.versionpublishedVersion
dspace.entity.typePublication
hcfmusp.author.externalAGUIAR, Ricardo Pinto:Univ Camilo Castelo Branco, Inst Biomed Engn, UNICASTELO, Sao Jose Dos Campos, SP, Brazil
hcfmusp.author.externalSILVEIRA JR., Landulfo:Univ Camilo Castelo Branco, Inst Biomed Engn, UNICASTELO, Sao Jose Dos Campos, SP, Brazil
hcfmusp.author.externalFALCAO, Edgar Teixeira:Hosp Sao Jose, Ilheus, BA, Brazil
hcfmusp.author.externalPACHECO, Marcos Tadeu Tavares:Univ Camilo Castelo Branco, Inst Biomed Engn, UNICASTELO, Sao Jose Dos Campos, SP, Brazil
hcfmusp.author.externalZANGARO, Renato Amaro:Univ Camilo Castelo Branco, Inst Biomed Engn, UNICASTELO, Sao Jose Dos Campos, SP, Brazil
hcfmusp.citation.scopus35
hcfmusp.contributor.author-fmusphcCARLOS AUGUSTO GONCALVES PASQUALUCCI
hcfmusp.description.beginpage595
hcfmusp.description.endpage604
hcfmusp.description.issue12
hcfmusp.description.volume31
hcfmusp.origemWOS
hcfmusp.origem.pubmed24251927
hcfmusp.origem.scopus2-s2.0-84890387175
hcfmusp.origem.wosWOS:000330527300006
hcfmusp.publisher.cityNEW ROCHELLE
hcfmusp.publisher.countryUSA
hcfmusp.relation.referenceBeleites C, 2011, ANAL BIOANAL CHEM, V400, P2801, DOI 10.1007/s00216-011-4985-4
hcfmusp.relation.referenceBeljebbar A, 2010, ANAL BIOANAL CHEM, V398, P477, DOI 10.1007/s00216-010-3910-6
hcfmusp.relation.referenceBlack P. E., 2004, EUCLIDEAN DISTANCE
hcfmusp.relation.referenceBodanese B, 2012, PHOTOMED LASER SURG, V30, P381, DOI 10.1089/pho.2011.3191
hcfmusp.relation.referenceBrien J. S., 1965, J LIPID RES, V6, P537
hcfmusp.relation.referenceBrien J. S., 1965, J LIPID RES, V6, P545
hcfmusp.relation.referenceChowdary MVP, 2007, PHOTOMED LASER SURG, V25, P269, DOI 10.1089/pho.2006.2066
hcfmusp.relation.referenceDreissig I, 2009, SPECTROCHIM ACTA A, V71, P2069, DOI 10.1016/j.saa.2008.08.008
hcfmusp.relation.referenceDunteman G. H., 1989, PRINCIPAL COMPONENTS
hcfmusp.relation.referenceHaka AS, 2009, J BIOMED OPT, V14, DOI 10.1117/1.3247154
hcfmusp.relation.referenceHalon E. B., 2000, PHYS MED BIOL, V45, pR1
hcfmusp.relation.referenceHarris A. T., 2009, HEAD NECK ONCOL, V1, P1
hcfmusp.relation.referenceHuang ZW, 2010, BIOSENS BIOELECTRON, V26, P383, DOI 10.1016/j.bios.2010.07.125
hcfmusp.relation.referenceInstituto Nacional de Cancer Jose Alencar Gomes da Silva-INCA, 2011, EST 2012 CANC INC BR
hcfmusp.relation.referenceJansen M. A., 2011, J BIOMED OPT, V16
hcfmusp.relation.referenceKanter EM, 2009, J RAMAN SPECTROSC, V40, P205, DOI 10.1002/jrs.2108
hcfmusp.relation.referenceKirsch M, 2010, ANAL BIOANAL CHEM, V398, P1707, DOI 10.1007/s00216-010-4116-7
hcfmusp.relation.referenceKohler M, 2009, ANAL BIOANAL CHEM, V393, P1513, DOI 10.1007/s00216-008-2592-9
hcfmusp.relation.referenceKoljenovic S, 2002, LAB INVEST, V82, P1265, DOI 10.1097/01.LAB.0000032545.96931.B8
hcfmusp.relation.referenceKrafft C, 2005, SPECTROCHIM ACTA A, V61, P1529, DOI 10.1016/j.saa.2004.11.017
hcfmusp.relation.referenceKrafft C, 2012, ANALYST, V137, P5533, DOI 10.1039/c2an36083g
hcfmusp.relation.referenceKrafft C, 2005, ANALYST, V130, P1070, DOI 10.1039/b419232j
hcfmusp.relation.referenceLeslie DG, 2012, PEDIATR NEUROSURG, V48, P109, DOI 10.1159/000343285
hcfmusp.relation.referenceLopes M. B. S., 2009, MENINGIOMAS DIAGNOSI, P25, DOI 10.1007/978-1-84628-784-8_4
hcfmusp.relation.referenceLouis DN, 2007, ACTA NEUROPATHOL, V114, P97, DOI 10.1007/s00401-007-0243-4
hcfmusp.relation.referenceMaheedhar K., 2011, PATHOLOG RES INT, V2011
hcfmusp.relation.referenceMcCreery R.L., 2000, RAMAN SPECTROSCOPY C
hcfmusp.relation.referenceMeyer T, 2011, J BIOMED OPT, V16, DOI 10.1117/1.3533268
hcfmusp.relation.referenceMontagnani S., 2000, Italian Journal of Anatomy and Embryology, V105, P167
hcfmusp.relation.referenceMovasaghi Z, 2007, APPL SPECTROSC REV, V42, P493, DOI 10.1080/05704920701551530
hcfmusp.relation.referenceNogueira GV, 2005, J BIOMED OPT, V10, DOI 10.1117/1.1908129
hcfmusp.relation.referenceOkamoto H, 2006, J PHASE EQUILIB DIFF, V27, P204, DOI 10.1361/154770306X97768
hcfmusp.relation.referenceQuarles R., 2005, BASIC NEUROCHEMISTRY, P51
hcfmusp.relation.referenceSchrader B., 1989, RAMAN INFRARED ATLAS
hcfmusp.relation.referenceOliveira AP, 2006, PHOTOMED LASER SURG, V24, P348, DOI 10.1089/pho.2006.24.348
hcfmusp.relation.referenceSilveira L, 2012, J BIOMED OPT, V17, DOI 10.1117/1.JBO.17.7.077003
hcfmusp.relation.referenceStone N, 2004, FARADAY DISCUSS, V126, P141, DOI 10.1039/b304992b
hcfmusp.relation.referenceStone N, 2007, ANAL BIOANAL CHEM, V387, P1657, DOI 10.1007/s00216-006-0937-9
hcfmusp.relation.referenceSUN GY, 1973, J LIPID RES, V14, P656
hcfmusp.relation.referenceSuzuki K., 1981, BASIC NEUROCHEMISTRY, P355
hcfmusp.relation.referenceTeh SK, 2010, BRIT J SURG, V97, P550, DOI 10.1002/bjs.6913
hcfmusp.relation.referenceVargis E, 2012, TRANSL ONCOL, V5, P172, DOI 10.1593/tlo.12106
hcfmusp.relation.referenceYATES AJ, 1979, J LIPID RES, V20, P428
hcfmusp.relation.referenceZhou Y, 2012, J BIOMED OPT, V17, DOI 10.1117/1.JBO.17.11.116021
hcfmusp.remissive.sponsorshipFAPESP
hcfmusp.scopus.lastupdate2024-05-10
relation.isAuthorOfPublication72f17805-b816-4624-98ba-73ed2144f830
relation.isAuthorOfPublication.latestForDiscovery72f17805-b816-4624-98ba-73ed2144f830
Arquivos
Pacote Original
Agora exibindo 1 - 1 de 1
Imagem de Miniatura
Nome:
art_PASQUALUCCI_Discriminating_Neoplastic_and_Normal_Brain_Tissues_in_Vitro_2013.PDF
Tamanho:
511.95 KB
Formato:
Adobe Portable Document Format
Descrição:
publishedVersion (English)
Baixar
Coleções
Artigos e Materiais de Revistas Científicas - FM/MPT
Artigos e Materiais de Revistas Científicas - LIM/22
Artigos e Materiais de Revistas Científicas - ODS/03