Genotyping of Toxoplasma gondii: DNA extraction from formalin-fixed paraffin-embedded autopsy tissues from AIDS patients who died by severe disseminated toxoplasmosis

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dc.contributorSistema FMUSP-HC: Faculdade de Medicina da Universidade de São Paulo (FMUSP) e Hospital das Clínicas da FMUSP
dc.contributor.authorSILVA, Inara Bastos da
dc.contributor.authorBATISTA, Tatiana Pimental de Andrade
dc.contributor.authorMARTINES, Roosecelis Brasil
dc.contributor.authorKANAMURA, Cristina Takami
dc.contributor.authorFERREIRA, Isabelle Martins Ribeiro
dc.contributor.authorVIDAL, Jose Ernesto
dc.contributor.authorPEREIRA-CHIOCCOLA, Vera Lucia
dc.date.accessioned2016-07-18T12:31:28Z
dc.date.available2016-07-18T12:31:28Z
dc.date.issued2016
dc.description.abstractThis study investigated the genetic features of Toxoplasma gondii isolated directly in autopsies of HIV-infected patients who died with severe disseminated toxoplasmosis. This retrospective analysis was conducted in a cohort of 15 HIV-infected patients with clinical and laboratory data. They had previous cerebral toxoplasmosis at least 6 months before the disseminated toxoplasmosis episode. The hypothesis was that they were infected with highly virulent parasites due to the condition in which they died. T. gondii genotyping was done directly in DNA extracted from 30 autopsy brain and lung samples (2 per patient) and mutilocus PCR-RFLP genotyping was done using 12 molecular markers. The 30 clinical samples were genotyped successfully in 8 or more loci and six suggestive genotypes were identified. One of them was Toxo DB #11, previously identified in different domestic animals and virulent in experimental animals. The other five suggestive genotypes identified in 14 patients were not described. TgHuDis1 was the most frequent and was determined in 8 patients. TgHuDis3 and TgHuDis5 were identified in two patients each. TgHuDis2 and TgHuDis4 have been identified in one patient each. These suggestive genotypes could be considered as virulent, since they caused severe tissue damage and had similar characteristics as Toxo # DB 11.
dc.description.indexMEDLINE
dc.description.sponsorshipFAPESP (Fundacao de Amparo a Pesquisa do Estado de Sao Paulo, Brazil) [2014/09496-1]
dc.description.sponsorshipCNPq (Conselho Nacional de Desenvolvimento Cientifico e Tecnologico, Brazil) [301369/2015-1]
dc.identifier.citationEXPERIMENTAL PARASITOLOGY, v.165, p.16-21, 2016
dc.identifier.doi10.1016/j.exppara.2016.03.004
dc.identifier.eissn1090-2449
dc.identifier.issn0014-4894
dc.identifier.urihttps://observatorio.fm.usp.br/handle/OPI/14456
dc.language.isoeng
dc.publisherACADEMIC PRESS INC ELSEVIER SCIENCE
dc.relation.ispartofExperimental Parasitology
dc.rightsrestrictedAccess
dc.rights.holderCopyright ACADEMIC PRESS INC ELSEVIER SCIENCE
dc.subjectDisseminated toxoplasmosis
dc.subjectBrazilian AIDS patients
dc.subjectToxoplasma gondii genotyping
dc.subject.othermultilocus pcr-rflp
dc.subject.othercongenital toxoplasmosis
dc.subject.othercerebral toxoplasmosis
dc.subject.otherocular toxoplasmosis
dc.subject.othergenetic diversity
dc.subject.otherclinical findings
dc.subject.othersao-paulo
dc.subject.otherbrazil
dc.subject.otherstrains
dc.subject.othersamples
dc.subject.wosParasitology
dc.titleGenotyping of Toxoplasma gondii: DNA extraction from formalin-fixed paraffin-embedded autopsy tissues from AIDS patients who died by severe disseminated toxoplasmosis
dc.typearticle
dc.type.categoryoriginal article
dc.type.versionpublishedVersion
dspace.entity.typePublication
hcfmusp.author.externalSILVA, Inara Bastos da:Ctr Parasitol & Micol, Lab Biol Mol Parasitas & Fungos, Sao Paulo, Brazil
hcfmusp.author.externalBATISTA, Tatiana Pimental de Andrade:Inst Infectol Emilio Ribas, Sao Paulo, Brazil
hcfmusp.author.externalMARTINES, Roosecelis Brasil:Adolfo Lutz Inst, Ctr Patol, BR-01246902 Sao Paulo, SP, Brazil
hcfmusp.author.externalKANAMURA, Cristina Takami:Adolfo Lutz Inst, Ctr Patol, BR-01246902 Sao Paulo, SP, Brazil
hcfmusp.author.externalFERREIRA, Isabelle Martins Ribeiro:Ctr Parasitol & Micol, Lab Biol Mol Parasitas & Fungos, Sao Paulo, Brazil; Supervisao Vigilancia Saude Santo Amaro Cidade Ad, Prefeitura Municipal Sao, Brazil
hcfmusp.author.externalPEREIRA-CHIOCCOLA, Vera Lucia:Ctr Parasitol & Micol, Lab Biol Mol Parasitas & Fungos, Sao Paulo, Brazil
hcfmusp.citation.scopus13
hcfmusp.contributor.author-fmusphcJOSE ERNESTO VIDAL BERMUDEZ
hcfmusp.description.beginpage16
hcfmusp.description.endpage21
hcfmusp.description.volume165
hcfmusp.origemWOS
hcfmusp.origem.pubmed26969767
hcfmusp.origem.scopus2-s2.0-84962828374
hcfmusp.origem.wosWOS:000376224400003
hcfmusp.publisher.citySAN DIEGO
hcfmusp.publisher.countryUSA
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Artigos e Materiais de Revistas Científicas - LIM/49
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