Transcriptome analysis of six tissues obtained post-mortem from sepsis patients

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art_SILVA_Transcriptome_analysis_of_six_tissues_obtained_postmortem_from_2023.PDF (2.4 MB)
Citações na Scopus
1
Tipo de produção
article
Data de publicação
2023
DOI
Scopus
Web of Science
PubMed
Título da Revista
ISSN da Revista
Título do Volume
Editora
WILEY
Autores
GONCALVES, Andre Nicolau Aquime
DIAS, Thomaz Luescher
BREDA, Cristiane Naffah Souza
BREDA, Leandro Carvalho Dantas
CAMARA, Niels Olsen Saraiva
NAKAYA, Helder I.
Citação
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE, v.27, n.20, p.3157-3167, 2023
Projetos de Pesquisa
Unidades Organizacionais
Fascículo
Resumo
Septic shock is a life-threatening clinical condition characterized by a robust immune inflammatory response to disseminated infection. Little is known about its impact on the transcriptome of distinct human tissues. To address this, we performed RNA sequencing of samples from the prefrontal cortex, hippocampus, heart, lung, kidney and colon of seven individuals who succumbed to sepsis and seven uninfected controls. We identified that the lungs and colon were the most affected organs. While gene activation dominated, strong inhibitory signals were also detected, particularly in the lungs. We found that septic shock is an extremely heterogeneous disease, not only when different individuals are investigated, but also when comparing different tissues of the same patient. However, several pathways, such as respiratory electron transport and other metabolic functions, revealed distinctive alterations, providing evidence that tissue specificity is a hallmark of sepsis. Strikingly, we found evident signals of accelerated ageing in our sepsis population.
Palavras-chave
cell response, immunity, sepsis, transcriptomics
URI
https://observatorio.fm.usp.br/handle/OPI/57658
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Coleções
Artigos e Materiais de Revistas Científicas - HC/ICHC
Artigos e Materiais de Revistas Científicas - LIM/05
Artigos e Materiais de Revistas Científicas - LIM/06
Artigos e Materiais de Revistas Científicas - LIM/51