Carnoy's solution is an adequate tissue fixative for routine surgical pathology, preserving cell morphology and molecular integrity

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dc.contributorSistema FMUSP-HC: Faculdade de Medicina da Universidade de São Paulo (FMUSP) e Hospital das Clínicas da FMUSP
dc.contributor.authorPEREIRA, Marina A.
dc.contributor.authorDIAS, Andre R.
dc.contributor.authorFARAJ, Sheila F.
dc.contributor.authorCIRQUEIRA, Cinthya dos S.
dc.contributor.authorTOMITAO, Michele T.
dc.contributor.authorNAHAS, Sergio Carlos
dc.contributor.authorRIBEIRO JR., Ulysses
dc.contributor.authorMELLO, Evandro S. de
dc.date.accessioned2015-07-01T20:22:37Z
dc.date.available2015-07-01T20:22:37Z
dc.date.issued2015
dc.description.abstractAims: To compare Carnoy's solution (CS) and 10% neutral buffered formalin solution (NBF) as tissue fixatives in colorectal cancer specimens. Methods and results: Surgical specimens from patients with colorectal cancer were analysed. Three groups were studied, as follows: group 1 consisted of 16 paired samples fixed in CS and NBF; and groups 2 and 3 consisted of 14 prospective and 80 retrospective samples, respectively, both randomized for fixation in CS or NBF. Groups 1 and 2 were analysed for amount, quality and integrity of DNA. Morphological analysis, including some of the usual special stains and polymerase chain reaction (PCR), were also performed for group 1, and Sanger sequencing for group 2. Immunohistochemical (IHC) reactions for mismatch repair proteins were studied in groups 1 and 3. Fixative performances were similar for morphology, special stains, and IHC reactions, as well as for the amount, quality and integrity of extracted DNA. PCR amplification was not possible in two cases from CS group 1. Sanger sequencing gave conclusive results for the CS samples tested. Conclusions: Carnoy's solution and NBF are equivalent fixatives for colorectal cancer specimens and are adequate for routine utilization in surgical and molecular pathology.
dc.description.indexMEDLINE
dc.description.sponsorshipCancer Institute, University of Sao Paulo School of Medicine, Sao Paulo, Brazil
dc.identifier.citationHISTOPATHOLOGY, v.66, n.3, p.388-397, 2015
dc.identifier.doi10.1111/his.12532
dc.identifier.eissn1365-2559
dc.identifier.issn0309-0167
dc.identifier.urihttps://observatorio.fm.usp.br/handle/OPI/9363
dc.language.isoeng
dc.publisherWILEY-BLACKWELL
dc.relation.ispartofHistopathology
dc.rightsrestrictedAccess
dc.rights.holderCopyright WILEY-BLACKWELL
dc.subjectCarnoy's solution
dc.subjectDNA extraction
dc.subjectformalin
dc.subjectimmunohistochemistry
dc.subjecttissue fixation
dc.subject.otherparaffin-embedded tissue
dc.subject.otherstaining techniques
dc.subject.otherclearing technique
dc.subject.othercancer
dc.subject.otherspecimens
dc.subject.otheramplification
dc.subject.otherexpression
dc.subject.otherformalin
dc.subject.othertime
dc.subject.otherpcr
dc.subject.wosCell Biology
dc.subject.wosPathology
dc.titleCarnoy's solution is an adequate tissue fixative for routine surgical pathology, preserving cell morphology and molecular integrity
dc.typearticle
dc.type.categoryoriginal article
dc.type.versionpublishedVersion
dspace.entity.typePublication
hcfmusp.citation.scopus28
hcfmusp.contributor.author-fmusphcMARINA ALESSANDRA PEREIRA
hcfmusp.contributor.author-fmusphcANDRE RONCON DIAS
hcfmusp.contributor.author-fmusphcSHEILA FRIEDRICH FARAJ
hcfmusp.contributor.author-fmusphcCINTHYA DOS SANTOS CIRQUEIRA BORGES
hcfmusp.contributor.author-fmusphcMICHELE TATIANA PEREIRA TOMITAO
hcfmusp.contributor.author-fmusphcSERGIO CARLOS NAHAS
hcfmusp.contributor.author-fmusphcULYSSES RIBEIRO JUNIOR
hcfmusp.contributor.author-fmusphcEVANDRO SOBROZA DE MELLO
hcfmusp.description.beginpage388
hcfmusp.description.endpage397
hcfmusp.description.issue3
hcfmusp.description.volume66
hcfmusp.origemWOS
hcfmusp.origem.pubmed25307771
hcfmusp.origem.scopus2-s2.0-84921521539
hcfmusp.origem.wosWOS:000348727200008
hcfmusp.publisher.cityHOBOKEN
hcfmusp.publisher.countryUSA
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