Repair genes expression profile of MLH1, MSH2 and ATM in the normal oral mucosa of chronic smokers

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dc.contributorSistema FMUSP-HC: Faculdade de Medicina da Universidade de São Paulo (FMUSP) e Hospital das Clínicas da FMUSP
dc.contributor.authorALVES, Monica Ghislaine Oliveira
dc.contributor.authorCARTA, Celina Faig Lima
dc.contributor.authorBARROSA, Patricia Pimentel de
dc.contributor.authorISSA, Jaqueline Scholz
dc.contributor.authorNUNES, Fabio Daumas
dc.contributor.authorALMEIDA, Janete Dias
dc.date.accessioned2017-04-07T15:14:32Z
dc.date.available2017-04-07T15:14:32Z
dc.date.issued2017
dc.description.abstractObjective: The aim of this study was to evaluate the effect of chronic smoking on the expression profile of the repair genes MLH1, MSH2 and ATM in the normal oral mucosa of chronic smokers and never smokers. Materials and methods: The sample consisted of thirty exfoliative cytology smears per group obtained from Smokers and Never Smokers. Total RNA was extracted and expression of the MLH1, MSH2 and ATM genes were evaluated by quantitative real-time and immunocytochemistry. The gene and protein expression data were correlated to the clinical data. Gene expression was analyzed statistically using the Student t-test and Pearson's correlation coefficient, with p < 0.05. Results: MLH1, MSH2 and ATM genes were downregulated in the smoking group compared to the control with significant values for MLH1 (p = 0.006), MSH2 (p = 0.0001) and ATM (p = 0.0001). Immunocytochemical staining for anti-MLH1, anti-MSH2 and anti-ATM was negative in Never Smokers; in Smokers it was rarely positive. No significant correlation was observed among the expression of MLH1, MSH2, ATM and age, number of cigarettes consumed per day, time of smoking during life, smoking history or levels of CO in expired air. Conclusion: The expression of genes and proteins related to DNA repair mechanism MLH1, MSH2 and ATM in the normal oral mucosa of chronic smokers was reduced.
dc.description.indexMEDLINE
dc.description.sponsorshipCoordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES)
dc.description.sponsorshipFundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [2012/05371-4, 2013/06251-5]
dc.identifier.citationARCHIVES OF ORAL BIOLOGY, v.73, p.60-65, 2017
dc.identifier.doi10.1016/j.archoralbio.2016.09.006
dc.identifier.eissn1879-1506
dc.identifier.issn0003-9969
dc.identifier.urihttps://observatorio.fm.usp.br/handle/OPI/19075
dc.language.isoeng
dc.publisherPERGAMON-ELSEVIER SCIENCE LTD
dc.relation.ispartofArchives of Oral Biology
dc.rightsrestrictedAccess
dc.rights.holderCopyright PERGAMON-ELSEVIER SCIENCE LTD
dc.subjectCarcinoma
dc.subjectSquamous cell
dc.subjectSmoking
dc.subjectGene expression immunocytochemistry
dc.subject.othersquamous-cell carcinoma
dc.subject.otherhuman mutl homolog-1
dc.subject.otherdna mismatch repair
dc.subject.otherreduced expression
dc.subject.othermicrosatellite instability
dc.subject.otherpromoter hypermethylation
dc.subject.otherexfoliative cytology
dc.subject.otherneck-cancer
dc.subject.otherhmlh1
dc.subject.otherhead
dc.subject.wosDentistry, Oral Surgery & Medicine
dc.titleRepair genes expression profile of MLH1, MSH2 and ATM in the normal oral mucosa of chronic smokers
dc.typearticle
dc.type.categoryoriginal article
dc.type.versionpublishedVersion
dspace.entity.typePublication
hcfmusp.author.externalALVES, Monica Ghislaine Oliveira:Univ Estadual Paulista, UNESP, Inst Sci & Technol, Dept Biosci & Oral Diag, Av Engn Francisco Jose Longo 777, BR-12245000 Sao Jose Dos Campos, SP, Brazil; Univ Braz Cubas, Sch Dent, Mogi Das Cruzes, Brazil
hcfmusp.author.externalCARTA, Celina Faig Lima:Univ Estadual Paulista, UNESP, Inst Sci & Technol, Dept Biosci & Oral Diag, Av Engn Francisco Jose Longo 777, BR-12245000 Sao Jose Dos Campos, SP, Brazil
hcfmusp.author.externalBARROSA, Patricia Pimentel de:Univ Estadual Paulista, UNESP, Inst Sci & Technol, Dept Biosci & Oral Diag, Av Engn Francisco Jose Longo 777, BR-12245000 Sao Jose Dos Campos, SP, Brazil
hcfmusp.author.externalNUNES, Fabio Daumas:Univ Sao Paulo, Sch Dent, Dept Oral Pathol, Sao Paulo, SP, Brazil
hcfmusp.author.externalALMEIDA, Janete Dias:Univ Estadual Paulista, UNESP, Inst Sci & Technol, Dept Biosci & Oral Diag, Av Engn Francisco Jose Longo 777, BR-12245000 Sao Jose Dos Campos, SP, Brazil
hcfmusp.citation.scopus7
hcfmusp.contributor.author-fmusphcJAQUELINE RIBEIRO SCHOLZ
hcfmusp.description.beginpage60
hcfmusp.description.endpage65
hcfmusp.description.volume73
hcfmusp.origemWOS
hcfmusp.origem.pubmed27693945
hcfmusp.origem.scopus2-s2.0-84988930984
hcfmusp.origem.wosWOS:000390823300010
hcfmusp.publisher.cityOXFORD
hcfmusp.publisher.countryENGLAND
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