Unusual remodeling of the hyalinization band in vulval lichen sclerosus by type V collagen and ECM 1 protein

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dc.contributor Sistema FMUSP-HC: Faculdade de Medicina da Universidade de São Paulo (FMUSP) e Hospital das Clínicas da FMUSP
dc.contributor.author GODOY, Charles A.P. FMUSP-HC
TEODORO, Walcy R. FMUSP-HC
VELOSA, Ana Paula P. FMUSP-HC
GARIPPO, Ana Lucia FMUSP-HC
EHER, Esmeralda Miristeni FMUSP-HC
PARRA, Edwin Roger FMUSP-HC
SOTTO, Mirian N. FMUSP-HC
CAPELOZZI, Vera L. FMUSP-HC
dc.date.issued 2015
dc.identifier.citation CLINICS, v.70, n.5, p.356-362, 2015
dc.identifier.issn 1807-5932
dc.identifier.uri http://observatorio.fm.usp.br/handle/OPI/10543
dc.description.abstract OBJECTIVES: The vulva is the primary site affected in lichen sclerosus, a chronic dermatosis in women that is histologically characterized by a zone of collagen remodeling in the superior dermis. The normal physiological properties of the vulva depend on the assembly of collagen types I (COLI), III (COLIII) and V (COLV), which form heterotypic fibers, and extracellular matrix protein interactions. COLV regulates the heterotypic fiber diameter, and the preservation of its properties is important for maintaining normal tissue architecture and function. In the current work, we analyzed the expression of COLV and its relationship with COLI, COLIII, elastic fibers and extracellular matrix protein 1 in vulvar biopsies from patients with lichen sclerosus. METHODS: Skin biopsies from 21 patients with lichen sclerosus, classified according to Hewitt histological criteria, were studied and compared to clinically normal vulvar tissue (N=21). Morphology, immunohistochemistry, immunofluorescence, 3D reconstruction and morphometric analysis of COLI, COLIII, COLV deposition, elastic fibers and extracellular matrix 1 expression in a zone of collagen remodeling in the superior dermis were performed. RESULTS: A significant decrease of elastic fibers and extracellular matrix 1 protein was present in the hyalinization zone of lichen sclerosus compared to healthy controls. The non-homogeneous distribution of collagen fibers visualized under immunofluorescence in the hyalinization zone of lichen sclerosus and control skin was confirmed by histomorphometry. Lichen sclerosus dermis shows a significant increase of COLI, COLIII and COLV expression compared to the healthy controls. Significant inverse associations were found between elastic fibers and COLV and between COLV and extracellular matrix 1 expression. A direct association was found between elastic fiber content and extracellular matrix 1 expression. Tridimensional reconstruction of the heterotypic fibers of the lichen sclerosus zone of collagen remodeling confirmed the presence of densely clustered COLV. CONCLUSIONS: Increased deposition of abnormal COLV and its correlation with extracellular matrix 1 and elastic fibers suggest that COLV may be a trigger in the pathogenesis of lichen sclerosus.
dc.description.sponsorship · Sao Paulo Research Foundation [FAPESP 2011/52095-0, FAPESP 2012/03543-2]
· National Center for Scientific and Technological Development [CNPq 471939/2010-2, CNPq 483005/2012-6, CNPq 300993/2010-2]
dc.language.iso eng
dc.publisher Faculdade de Medicina / USP
dc.relation.ispartof Clinics
dc.rights openAccess
dc.subject Vulvar lichen sclerosus; Collagen V; ECM 1 protein, Remodeling
dc.subject.other EXTRACELLULAR-MATRIX PROTEIN-1; ET-ATROPHICUS; SYSTEMIC-SCLEROSIS; LUNG TRANSPLANTATION; EXPERIMENTAL-MODEL; T-CELLS; SKIN; AUTOANTIBODIES; PATHOGENESIS; DEPOSITION
dc.title Unusual remodeling of the hyalinization band in vulval lichen sclerosus by type V collagen and ECM 1 protein
dc.type article
dc.rights.holder Copyright Faculdade de Medicina / USP
dc.description.group LIM/17
dc.description.group LIM/53
dc.identifier.doi 10.6061/clinics/2015(05)09
dc.identifier.pmid 26039953
dc.type.category original article
dc.type.version publishedVersion
hcfmusp.author GODOY, Charles A.P.:FM:
hcfmusp.author TEODORO, Walcy R.:FM:MCM
hcfmusp.author VELOSA, Ana Paula P.:HC:LIM/17
hcfmusp.author GARIPPO, Ana Lucia:FM:MPR
hcfmusp.author EHER, Esmeralda Miristeni:HC:ICHC
hcfmusp.author PARRA, Edwin Roger:FM:
hcfmusp.author SOTTO, Mirian N.:FM:MDT
hcfmusp.author CAPELOZZI, Vera L.:FM:MPT
hcfmusp.origem.id 2-s2.0-84930517230
hcfmusp.origem.id WOS:000355800500009
hcfmusp.origem.id SCIELO:S1807-59322015000500356
hcfmusp.publisher.city São Paulo
hcfmusp.publisher.country BRAZIL
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dc.description.index MEDLINE
dc.identifier.eissn 1980-5322
hcfmusp.citation.scopus 5
hcfmusp.citation.wos 4


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