MicroRNA Transcriptome Profiling in Heart of Trypanosoma cruzi-Infected Mice: Parasitological and Cardiological Outcomes
Carregando...
Citações na Scopus
53
Tipo de produção
article
Data de publicação
2015
Título da Revista
ISSN da Revista
Título do Volume
Editora
PUBLIC LIBRARY SCIENCE
Autores
NAKAYA, Helder I.
VILAR-PEREIRA, Glaucia
PEREIRA, Isabela Resende
REAL, Juliana Monte
CHEVILLARD, Christophe
Citação
PLOS NEGLECTED TROPICAL DISEASES, v.9, n.6, article ID e0003828, 17p, 2015
Resumo
Chagas disease is caused by the parasite Trypanosoma cruzi, and it begins with a short acute phase characterized by high parasitemia followed by a life-long chronic phase with scarce parasitism. Cardiac involvement is the most prominent manifestation, as 30% of infected subjects will develop abnormal ventricular repolarization with myocarditis, fibrosis and cardiomyocyte hypertrophy by undefined mechanisms. Nevertheless, follow-up studies in chagasic patients, as well as studies with murine models, suggest that the intensity of clinical symptoms and pathophysiological events that occur during the acute phase of disease are associated with the severity of cardiac disease observed during the chronic phase. In the present study we investigated the role of microRNAs (miRNAs) in the disease progression in response to T. cruzi infection, as alterations in miRNA levels are known to be associated with many cardiovascular disorders. We screened 641 rodent miRNAs in heart samples of mice during an acute infection with the Colombiana T.cruzi strain and identified multiple miRNAs significantly altered upon infection. Seventeen miRNAs were found significantly deregulated in all three analyzed time points post infection. Among these, six miRNAs had their expression correlated with clinical parameters relevant to the disease, such as parasitemia and maximal heart rate-corrected QT (QTc) interval. Computational analyses identified that the gene targets for these six miRNAs were involved in networks and signaling pathways related to increased ventricular depolarization and repolarization times, important factors for QTc interval prolongation. The data presented here will guide further studies about the contribution of microRNAs to Chagas heart disease pathogenesis.
Palavras-chave
Referências
- Alvarado-Tapias E, 2012, INVEST CLIN, V53, P378
- BENJAMINI Y, 1995, J ROY STAT SOC B MET, V57, P289
- Bern C, 2011, ADV PARASIT, V75, P19, DOI 10.1016/B978-0-12-385863-4.00002-2
- Bilate AM, 2008, J INFECT DIS, V198, P614, DOI 10.1086/590347
- Bilate AMB, 2008, REV INST MED TROP SP, V50, P67, DOI 10.1590/S0036-46652008000200001
- Bostjancic E, 2010, FOLIA BIOL-PRAGUE, V56, P27
- BRENER Z., 1962, REV INST MED TROP SAO PAULO, V4, P389
- Caldas IS, 2008, PARASITOL RES, V103, P413, DOI 10.1007/s00436-008-0992-6
- Camandaroba Edson Luiz Paes, 2001, Revista da Sociedade Brasileira de Medicina Tropical, V34, P151
- Eickhoff CS, 2010, J PARASITOL, V96, P758, DOI 10.1645/GE-2396.1
- Ferreira LRP, 2014, INT J CARDIOL, V175, P409, DOI 10.1016/j.ijcard.2014.05.019
- Friedman RC, 2009, GENOME RES, V19, P92, DOI 10.1101/gr.082701.108
- Gibson UEM, 1996, GENOME RES, V6, P995, DOI 10.1101/gr.6.10.995
- Gollob MH, 2006, NEW ENGL J MED, V354, P2677, DOI 10.1056/NEJMoa052800
- Itokawa K, 1999, MOL BRAIN RES, V71, P354, DOI 10.1016/S0169-328X(99)00194-1
- Latronico MVG, 2009, NAT REV CARDIOL, V6, P418, DOI 10.1038/nrcardio.2009.56
- Lee CT, 2007, DNA CELL BIOL, V26, P209, DOI 10.1089/dna.2006.0545
- LEE RC, 1993, CELL, V75, P843, DOI 10.1016/0092-8674(93)90529-Y
- Marinho CRF, 1999, INFECT IMMUN, V67, P308
- Moncayo A, 2006, ANN TROP MED PARASIT, V100, P663, DOI 10.1179/136485906X112248
- Newton-Cheh C, 2009, NAT GENET, V41, P399, DOI 10.1038/ng.364
- Oliveira-Carvalho V, 2012, ARQ BRAS CARDIOL, V98, P362, DOI 10.1590/S0066-782X2012000400011
- Rao PK, 2009, CIRC RES, V105, P585, DOI 10.1161/CIRCRESAHA.109.200451
- Rassi A, 2012, INFECT DIS CLIN N AM, V26, P275, DOI 10.1016/j.idc.2012.03.002
- Ribeiro AL, 2013, PLOS NEGLECT TROP D, V7, DOI 10.1371/journal.pntd.0002078
- Roman-Campos D, 2013, MEM I OSWALDO CRUZ, V108, P243
- Roman-Campos D, 2009, BASIC RES CARDIOL, V104, P238, DOI 10.1007/s00395-009-0776-x
- Salles G, 2003, CIRCULATION, V108, P305, DOI 10.1161/01.CIR.00000749174.13444.9C
- Salles GF, 2004, AM J CARDIOL, V93, P1136, DOI 10.1016/j.amjcard.2004.01.040
- Salles GF, 2003, PACE, V26, P1326, DOI 10.1046/j.1460-9592.2003.t01-1-00190.x
- Sayed D, 2007, CIRC RES, V100, P416, DOI 10.1161/01.RES.0000257913.42552.23
- Schmittgen TD, 2008, NAT PROTOC, V3, P1101, DOI 10.1038/nprot.2008.73
- Silverio JC, 2012, PLOS PATHOG, V8, DOI 10.1371/journal.ppat.1002645
- Smyth G. K., 2004, APPL GENET MOL BIOL, V3, DOI 10.2202/1544-6115.1027
- Talvani A, 2000, MICROBES INFECT, V2, P851, DOI 10.1016/S1286-4579(00)00388-9
- Teixeira MM, 2002, TRENDS PARASITOL, V18, P262, DOI 10.1016/S1471-4922(02)02283-3
- Wulff H, 2009, NAT REV DRUG DISCOV, V8, P982, DOI 10.1038/nrd2983
Coleções
Artigos e Materiais de Revistas Científicas - FM/MPT
Artigos e Materiais de Revistas Científicas - FM/MCM
Artigos e Materiais de Revistas Científicas - HC/ICHC
Artigos e Materiais de Revistas Científicas - HC/InCor
Artigos e Materiais de Revistas Científicas - LIM/06
Artigos e Materiais de Revistas Científicas - LIM/19
Carregar mais Artigos e Materiais de Revistas Científicas - FM/MCM
Artigos e Materiais de Revistas Científicas - HC/ICHC
Artigos e Materiais de Revistas Científicas - HC/InCor
Artigos e Materiais de Revistas Científicas - LIM/06
Artigos e Materiais de Revistas Científicas - LIM/19