Please use this identifier to cite or link to this item: https://observatorio.fm.usp.br/handle/OPI/12629
Title: Durable sequence stability and bone marrow tropism in a macaque model of human pegivirus infection
Authors: BAILEY, Adam L.LAUCK, MichaelMOHNS, MarielPETERSON, Eric J.BEHELER, KerryBRUNNER, Kevin G.CROSNO, KristinMEJIA, AndresMUTSCHLER, JamesGEHRKE, MatthewGREENE, JustinERICSEN, Adam J.WEILER, AndreaLEHRER-BREY, GabrielleFRIEDRICH, Thomas C.SIBLEY, Samuel D.KALLAS, Esper G.III, Saverio CapuanoROGERS, JeffreyGOLDBERG, Tony L.SIMMONS, Heather A.O'CONNOR, David H.
Citation: SCIENCE TRANSLATIONAL MEDICINE, v.7, n.305, article ID 305ra144, 11p, 2015
Abstract: Human pegivirus (HPgV)-formerly known as GB virus C and hepatitis G virus-is a poorly characterized RNA virus that infects about one-sixth of the global human population and is transmitted frequently in the blood supply. We create an animal model of HPgV infection by infecting macaque monkeys with a new simian pegivirus (SPgV) discovered in wild baboons. Using this model, we provide a high-resolution, longitudinal picture of SPgV viremia where the dose, route, and timing of infection are known. We detail the highly variable acute phase of SPgV infection, showing that the viral load trajectory early in infection is dependent on the infecting dose, whereas the chronic-phase viremic set point is not. We also show that SPgV has an extremely low propensity for accumulating sequence variation, with no consensus-level variants detected during the acute phase of infection and an average of only 1.5 variants generated per 100 infection-days. Finally, we show that SPgV RNA is highly concentrated in only two tissues: spleen and bone marrow, with bone marrow likely producing most of the virus detected in plasma. Together, these results reconcile several paradoxical observations from cross-sectional analyses of HPgV in humans and provide an animal model for studying pegivirus biology.
Appears in Collections:

Artigos e Materiais de Revistas Científicas - FM/MCM
Departamento de Clínica Médica - FM/MCM

Artigos e Materiais de Revistas Científicas - HC/ICHC
Instituto Central - HC/ICHC

Artigos e Materiais de Revistas Científicas - LIM/60
LIM/60 - Laboratório de Imunologia Clínica e Alergia

Artigos e Materiais de Revistas Científicas - ODS/03
ODS/03 - Saúde e bem-estar


Files in This Item:
File Description SizeFormat 
art_BAILEY_Durable_sequence_stability_and_bone_marrow_tropism_in_2015.PDF
  Restricted Access
publishedVersion (English)643.72 kBAdobe PDFView/Open Request a copy

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.