HEV infection in swine from Eastern Brazilian Amazon: Evidence of co-infection by different subtypes

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dc.contributor Sistema FMUSP-HC: Faculdade de Medicina da Universidade de São Paulo (FMUSP) e Hospital das Clínicas da FMUSP
dc.contributor.author SOUZA, Alex Junior Souza de
SOARES, Manoel do Carmo Pereira
PINHO, Joao Renato Rebello FMUSP-HC
MALHEIROS, Andreza Pinheiro
CARNEIRO, Liliane Almeida
SANTOS, Debora Regina Lopes dos
PEREIRA, Washington Luiz Assuncao
dc.date.issued 2012
dc.identifier.citation COMPARATIVE IMMUNOLOGY MICROBIOLOGY AND INFECTIOUS DISEASES, v.35, n.5, p.477-485, 2012
dc.identifier.issn 0147-9571
dc.identifier.uri http://observatorio.fm.usp.br/handle/OPI/1274
dc.description.abstract Hepatitis E virus (HEV) is a fecal-orally transmitted member of the genus Hepevirus that causes acute hepatitis in humans and is widely distributed throughout the world. Pigs have been reported as the main source of genotypes 3 and 4 infection to humans in non-endemic areas. To investigate HEV infection in pigs from different regions of Para state (Eastern Brazilian Amazon), we performed serological and molecular analyses of serum, fecal and liver samples from 151 adult pigs slaughtered between April and October 2010 in slaughterhouses in the metropolitan region of Belem, Para. Among the animals tested, 8.6% (13/151) were positive for anti-HEV IgG but not for anti-HEV IgM. HEV RNA was detected in 4.8% (22/453) of the samples analyzed and 9.9% (15/151) of the animals had at least one positive sample. Phylogenetic analysis showed that all sequences belonged to genotype 3 that were related to human isolates from other non-endemic regions, suggesting that the isolates had zoonotic potential. Subtypes 3c and 3f were simultaneously detected in some pigs, suggesting co-infection by more than one strain and/or the presence of a recombinant virus. These results constitute the first molecular and serologic evidence of swine HEV circulation in the Eastern Brazilian Amazon.
dc.description.sponsorship · Instituto Evandro Chagas/Secretaria de Vigilancia em Saude/Ministerio da Saude
· Coordenacao de aperfeicoamento de pessoal de nivel superior - CAPES/Universidade Federal do Para - UFPA
· Fundacao de Amparo a Pesquisa do Estado de Sao Paulo - FAPESP [2010/50081-9, 2009/53946-3]
dc.language.iso eng
dc.publisher ELSEVIER SCI LTD
dc.relation.ispartof Comparative Immunology Microbiology and Infectious Diseases
dc.rights restrictedAccess
dc.subject Hepatitis E virus (HEV); Pigs; Zoonosis; Genotype 3; Brazil
dc.subject.other hepatitis-e-virus; genetic-characterization; pig-liver; avian hev; genotype; prevalence; identification; antibodies; sequence; japan
dc.title HEV infection in swine from Eastern Brazilian Amazon: Evidence of co-infection by different subtypes
dc.type article
dc.rights.holder Copyright ELSEVIER SCI LTD
dc.description.group LIM/07
dc.identifier.doi 10.1016/j.cimid.2012.04.004
dc.identifier.pmid 22579295
dc.type.category original article
dc.type.version publishedVersion
hcfmusp.author GOMES-GOUVEA, Michele Soares:FM:
hcfmusp.author PINHO, Joao Renato Rebello:HC:ICHC
hcfmusp.author.external · SOUZA, Alex Junior Souza de:Inst Evandro Chagas, Secao Hepatol, BR-66093020 Belem, PA, Brazil; Fac Integradas Tapajos, Ctr Ciencias Biol & Saude, BR-68010200 Santarem, PA, Brazil
· SOARES, Manoel do Carmo Pereira:Inst Evandro Chagas, Secao Hepatol, BR-66093020 Belem, PA, Brazil
· MALHEIROS, Andreza Pinheiro:Inst Evandro Chagas, Secao Hepatol, BR-66093020 Belem, PA, Brazil
· CARNEIRO, Liliane Almeida:Inst Evandro Chagas, Secao Hepatol, BR-66093020 Belem, PA, Brazil
· SANTOS, Debora Regina Lopes dos:Univ Fed Rural Rio de Janeiro, Inst Vet, BR-23890000 Rio De Janeiro, RJ, Brazil
· PEREIRA, Washington Luiz Assuncao:Univ Fed Rural Amazonia, Inst Saude & Prod Anim, BR-66077901 Belem, PA, Brazil
hcfmusp.origem.id 2-s2.0-84864115833
hcfmusp.origem.id WOS:000307616000009
hcfmusp.publisher.city OXFORD
hcfmusp.publisher.country ENGLAND
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dc.description.index MEDLINE
hcfmusp.citation.scopus 27
hcfmusp.citation.wos 25
hcfmusp.affiliation.country Brasil

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