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Title: | Antibodies are not required to a protective immune response against dengue virus elicited in a mouse encephalitis model |
Authors: | AMORIM, Jaime Henrique; ALVES, Rubens Prince dos Santos; BIZERRA, Raiza; PEREIRA, Sara Araujo; PEREIRA, Lennon Ramos; FABRIS, Denicar Lina Nascimento; SANTOS, Robert Andreata; ROMANO, Camila Malta; FERREIRA, Luis Carlos de Souza |
Citation: | VIROLOGY, v.487, p.41-49, 2016 |
Abstract: | Generating neutralizing antibodies have been considered a prerequisite to control dengue virus (DENV) infection. However, T lymphocytes have also been shown to be important in a protective immune state. In order to investigate the contribution of both humoral and cellular immune responses in DENV immunity, we used an experimental model in which a non-lethal DENV2 strain (ACS46) is used to intracranially prime Balb/C mice which develop protective immunity against a lethal DENV2 strain (JHA1). Primed mice generated envelope-specific antibodies and CD8(+) T cell responses targeting mainly non-structural proteins. Immune sera from protected mice did not confer passive protection to naive mice challenged with the JHA1 strain. In contrast, depletion of CD4(+) and CD8(+) T lymphocytes significantly reduced survival of ACS46-primed mice challenged with the JHA1 strain. Collectively, results presented in this study show that a cellular immune response targeting non-structural proteins are a promising way in vaccine development against dengue. |
Appears in Collections: | Artigos e Materiais de Revistas Científicas - LIM/52 Artigos e Materiais de Revistas Científicas - ODS/03 |
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