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https://observatorio.fm.usp.br/handle/OPI/14424
Title: | Combination of Bioactive Polymeric Membranes and Stem Cells for Periodontal Regeneration: In Vitro and In Vivo Analyses |
Authors: | GONCALVES, Flavia; MORAES, Miriam Santos de; FERREIRA, Lorraine Braga; CARREIRA, Ana Claudia Oliveira; KOSSUGUE, Patricia Mayumi; BOARO, Leticia Cristina Cidreira; BENTINI, Ricardo; GARCIA, Celia Regina da Silva; SOGAYAR, Mari Cleide; ARANA-CHAVEZ, Victor Elias; CATALANI, Luiz Henrique |
Citation: | PLOS ONE, v.11, n.3, article ID e0152412, 21p, 2016 |
Abstract: | Regeneration of periodontal tissues requires a concerted effort to obtain consistent and predictable results in vivo. The aim of the present study was to test a new family of bioactive polymeric membranes in combination with stem cell therapy for periodontal regeneration. In particular, the novel polyester poly(isosorbide succinate-co-L-lactide) (PisPLLA) was compared with poly(L-lactide) (PLLA). Both polymers were combined with collagen (COL), hydroxyapatite (HA) and the growth factor bone morphogenetic protein-7 (BMP7), and their osteoinductive capacity was evaluated via in vitro and in vivo experiments. Membranes composed of PLLA/COL/HA or PisPLLA/COL/HA were able to promote periodontal regeneration and new bone formation in fenestration defects in rat jaws. According to quantitative real-time polymerase chain reaction (qRT-PCR) and Alizarin Red assays, better osteoconductive capacity and increased extracellular mineralization were observed for PLLA/COL/HA, whereas better osteoinductive properties were associated with PisPLLA/COL/HA. We concluded that membranes composed of either PisPLLA/COL/HA or PLLA/COL/HA present promising results in vitro as well as in vivo and that these materials could be potentially applied in periodontal regeneration. |
Appears in Collections: | Artigos e Materiais de Revistas Científicas - FM/Outros |
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art_GONCALVES_Combination_of_Bioactive_Polymeric_Membranes_and_Stem_Cells_2016.PDF | publishedVersion (English) | 1.65 MB | Adobe PDF | View/Open |
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