Please use this identifier to cite or link to this item: https://observatorio.fm.usp.br/handle/OPI/14424
Title: Combination of Bioactive Polymeric Membranes and Stem Cells for Periodontal Regeneration: In Vitro and In Vivo Analyses
Authors: GONCALVES, FlaviaMORAES, Miriam Santos deFERREIRA, Lorraine BragaCARREIRA, Ana Claudia OliveiraKOSSUGUE, Patricia MayumiBOARO, Leticia Cristina CidreiraBENTINI, RicardoGARCIA, Celia Regina da SilvaSOGAYAR, Mari CleideARANA-CHAVEZ, Victor EliasCATALANI, Luiz Henrique
Citation: PLOS ONE, v.11, n.3, article ID e0152412, 21p, 2016
Abstract: Regeneration of periodontal tissues requires a concerted effort to obtain consistent and predictable results in vivo. The aim of the present study was to test a new family of bioactive polymeric membranes in combination with stem cell therapy for periodontal regeneration. In particular, the novel polyester poly(isosorbide succinate-co-L-lactide) (PisPLLA) was compared with poly(L-lactide) (PLLA). Both polymers were combined with collagen (COL), hydroxyapatite (HA) and the growth factor bone morphogenetic protein-7 (BMP7), and their osteoinductive capacity was evaluated via in vitro and in vivo experiments. Membranes composed of PLLA/COL/HA or PisPLLA/COL/HA were able to promote periodontal regeneration and new bone formation in fenestration defects in rat jaws. According to quantitative real-time polymerase chain reaction (qRT-PCR) and Alizarin Red assays, better osteoconductive capacity and increased extracellular mineralization were observed for PLLA/COL/HA, whereas better osteoinductive properties were associated with PisPLLA/COL/HA. We concluded that membranes composed of either PisPLLA/COL/HA or PLLA/COL/HA present promising results in vitro as well as in vivo and that these materials could be potentially applied in periodontal regeneration.
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