HPV16 E6 regulates annexin 1 (ANXA1) protein expression in cervical carcinoma cell lines

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art_CALMON_HPV16_E6_regulates_annexin_1_ANXA1_protein_expression_2016.PDF (720.06 KB)
Citações na Scopus
11
Tipo de produção
article
Data de publicação
2016
Editora
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI
Indexadores
Scopus
Web of Science
PubMed
Título da Revista
ISSN da Revista
Título do Volume
Autores
CALMON, Marilia Freitas
BOCCARDO, Enrique
RAHAL, Paula
Autor de Grupo de pesquisa
Editores
Coordenadores
Organizadores
Citação
VIROLOGY, v.496, p.35-41, 2016
Projetos de Pesquisa
Unidades Organizacionais
Fascículo
Resumo
Annexin 1 (ANXA1) is a substrate for E6AP mediated ubiquitylation. It has been hypothesized that HPV 16 E6 protein redirects E6AP away from ANXA1, increasing its stability and possibly contributing to viral pathogenesis. We analyzed ANXA1 expression in HPV-positive and negative cervical carcinoma-derived cells, in cells expressing HPV-16 oncogenes and in cells transduced with shRNA targeting E6AP. We observed that ANXA1 protein expression increased in HPV-16-positive tumor cells, in keratinocytes expressing HPV-16 E6wt (wild-type) or E6/E7 and C33 cells expressing HPV-16 E6wt. ANXA1 protein expression decreased in cells transfected with E6 Dicer-substrate RNAs (DsiRNA) and 03 cells co-transduced with HPV-16 E6wt and E6AP shRNA. Moreover, colony number and proliferation rate decreased in HPV16-positive cells transduced with ANXA1 shRNA. We observed that in cells infected with HPV16, the E6 binds to E6AP to degrade p53 and upregulate ANXA1. We suggest that ANXA1 may play a role in HPV-mediated carcinogenesis.
Palavras-chave
HPV16, E6, ANXA1, P53, Cervical cell lines, Keratinocytes
URI
https://observatorio.fm.usp.br/handle/OPI/16162
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Coleções
Artigos e Materiais de Revistas Científicas - FM/MDR
Artigos e Materiais de Revistas Científicas - HC/ICESP
Artigos e Materiais de Revistas Científicas - LIM/24
Artigos e Materiais de Revistas Científicas - ODS/03