Effects of caffeine supplementation in post-thaw human semen over different incubation periods
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17
Tipo de produção
article
Data de publicação
2016
Editora
WILEY-BLACKWELL
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Citação
ANDROLOGIA, v.48, n.9, p.961-966, 2016
Resumo
This study aimed to evaluate the effects of caffeine supplementation in post-cryopreservation human semen over different incubation periods. After collection by masturbation, 17 semen samples were analysed according to World Health Organization criteria, processed and cryopreserved with TEST-yolk buffer (1:1) in liquid nitrogen. After a thawing protocol, samples were incubated with 2mm of caffeine for 0, 5, 15, 30 or 60min, followed by analysis of motility and mitochondrial activity using 3,3-diaminobenzidine (DAB). Mean variance analysis was performed, and P<0.05 was the adopted significance threshold. Samples incubated for 15min showed increased progressive motility compared to other periods of incubation, as well as a reduced percentage of immotile spermatozoa (P<0.05). In samples incubated for 5min, increased mitochondrial activity above 50% was observed (DABI and DABII). Although cryosurvival rates were low after the cryopreservation process, incubation with caffeine was associated with an increase in sperm motility, particularly 15-min incubation, suggesting that incubation with caffeine can be an important tool in patients with worsening seminal quality undergoing infertility treatment.
Palavras-chave
Caffeine, cryopreservation, mitochondria, sperm motility, spermatozoa
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