High performance mass spectrometry based proteomics reveals enzyme and signaling pathway regulation in neutrophils during the early stage of surgical trauma

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art_ARSHID_High_performance_mass_spectrometry_based_proteomics_reveals_enzyme_2017.PDF (509.74 KB)
Citações na Scopus
15
Tipo de produção
article
Data de publicação
2017
Editora
WILEY-V C H VERLAG GMBH
DOI
Indexadores
Scopus
Web of Science
PubMed
Título da Revista
ISSN da Revista
Título do Volume
Autores
TAHIR, Muhammad
CASTRO, Mariana S.
SIDOLI, Simone
ROEPSTORFF, Peter
FONTES, Wagner
Autor de Grupo de pesquisa
Editores
Coordenadores
Organizadores
Citação
PROTEOMICS CLINICAL APPLICATIONS, v.11, n.1-2, article ID UNSP 1600001, 16p, 2017
Projetos de Pesquisa
Unidades Organizacionais
Fascículo
Resumo
Purpose: In clinical conditions trauma is associated with high mortality and morbidity. Neutrophils play a key role in the development of multiple organ failure after trauma Experimental design: To have a detailed understanding of the neutrophil activation at primary stages after trauma, neutrophils are isolated from control and surgical trauma rats in this study. Extracted proteins are analyzed using nano liquid chromatography coupled with tandem mass spectrometry. Results: A total of 2924 rat neutrophil proteins are identified in our analysis, of which 393 are found differentially regulated between control and trauma groups. By using functional pathways analysis of the 190 proteins up-regulated in surgical trauma, we found proteins related to transcription initiation and protein biosynthesis. On the other hand, among the 203 proteins down-regulated in surgical trauma we found enrichment for proteins of the immune response, proteasome degradation and actin cytoskeleton. Overall, enzyme prediction analysis revealed that regulated enzymes are directly involved in neutrophil apoptosis, directional migration and chemotaxis. Our observations are then confirmed by in silico protein-protein interaction analysis. Conclusions and clinical relevance: Collectively, our results reveal that neutrophils drastically regulate their biochemical pathways after the early stages of surgical trauma, showing lower activity. This implies higher susceptibility of the trauma patients to infection and bystander tissues damage.
Palavras-chave
Inflammation, Neutrophil, Proteome, Surgery, Trauma
URI
https://observatorio.fm.usp.br/handle/OPI/18715
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Coleções
Artigos e Materiais de Revistas Científicas - FM/MCG
Artigos e Materiais de Revistas Científicas - LIM/62