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https://observatorio.fm.usp.br/handle/OPI/18803
Title: | Platelet-activating factor (PAF) receptor as a promising target for cancer cell repopulation after radiotherapy |
Authors: | SILVA JR., I. A. da; CHAMMAS, R.; LEPIQUE, A. P.; JANCAR, S. |
Citation: | ONCOGENESIS, v.6, article ID e296, 9p, 2017 |
Abstract: | A major drawback of radiotherapy is the accelerated growth of the surviving tumor cells. Radiotherapy generates a variety of lipids that bind to the receptor for platelet-activating factor, expressed by cells in the tumor microenvironment. In the present study, using the TC-1 tumor cell line, we found that irradiation induced a twofold increase in receptor expression and generated agonists of receptor. Irradiated cells induced a 20-fold increase in live TC-1 proliferation in vitro. Furthermore, subcutaneous co-injection of irradiated TC-1 cells with TC-1 expressing luciferase (TC-1 fluc(+)) markedly increased TC-1 fluc(+) proliferation in a receptor-dependent way. Moreover we used a human carcinoma cell line not expressing the PAF receptor (KBM) and the same cell transfected with the receptor gene (KBP). Following co-injection of live KBP cells with irradiated KBM in RAG mice, the tumor growth was significantly increased compared with tumor formed following co-injection of live KBM with irradiated KBM. This tumor cell repopulation correlated with increased infiltration of tumor-promoting macrophages (CD206+). We propose that receptor represents a possible target for improving the efficacy of radiotherapy through inhibition of tumor repopulation. |
Appears in Collections: | Artigos e Materiais de Revistas Científicas - FM/MDR Artigos e Materiais de Revistas Científicas - LIM/24 Artigos e Materiais de Revistas Científicas - ODS/03 |
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art_SILVA JR._Plateletactivating_factor_PAF_receptor_as_a_promising_target_2017.PDF | publishedVersion (English) | 2.23 MB | Adobe PDF | View/Open |
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