Potential immunological markers for diagnosis of human strongyloidiasis using heterologous antigens

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art_CORRAL_Potential_immunological_markers_for_diagnosis_of_human_strongyloidiasis_2017.PDF (207.88 KB)
Citações na Scopus
16
Tipo de produção
article
Data de publicação
2017
Editora
CAMBRIDGE UNIV PRESS
DOI
Indexadores
Scopus
Web of Science
PubMed
Título da Revista
ISSN da Revista
Título do Volume
Autores
CHIEFFI, P. P.
CASTRO-BORGES, W.
Autor de Grupo de pesquisa
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Citação
PARASITOLOGY, v.144, n.2, p.124-130, 2017
Projetos de Pesquisa
Unidades Organizacionais
Fascículo
Resumo
Strongyloides venezuelensis is a parasitic nematode of rodents that is frequently used to obtain heterologous antigens for immunological diagnosis of human strongyloidiasis. The aim of this study was to identify antigens from filariform larvae of S. venezuelensis for immunodiagnosis of human strongyloidiasis. Soluble and membrane fractions from filariform larvae of S. venezuelensis were obtained in phosphate saline (SS and SM) and in Tris-HCl buffer (TS and TM), and were analysed by Western blotting. Different antigenic components were recognized by IgG antibodies from the sera of strongyloidiasis patients. Highest recognition was observed for a 30-40 kDa mass range present in all antigenic fractions. The band encompassing this mass range was then excised and subjected to mass spectrometry for protein identification. Immunoreactive proteins identified in the soluble fractions corresponded to metabolic enzymes, whereas cytoskeletal proteins and galectins were more abundant in the membrane fractions. These results represent the first approach towards identification of S. venezuelensis antigens for use in immunodiagnostic assays for human strongyloidiasis.
Palavras-chave
Strongyloides venezuelensis, immunodiagnostic, antigens, actin, galectin
URI
https://observatorio.fm.usp.br/handle/OPI/19949
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Coleções
Artigos e Materiais de Revistas Científicas - FM/MCM
Artigos e Materiais de Revistas Científicas - FM/MIP
Artigos e Materiais de Revistas Científicas - HC/ICHC
Artigos e Materiais de Revistas Científicas - LIM/03
Artigos e Materiais de Revistas Científicas - LIM/06
Artigos e Materiais de Revistas Científicas - LIM/31