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https://observatorio.fm.usp.br/handle/OPI/20043
Title: | Connexin32 deficiency is associated with liver injury, inflammation and oxidative stress in experimental non-alcoholic steatohepatitis |
Authors: | TIBURCIO, Tayna Cristina; WILLEBRORDS, Joost; SILVA, Tereza Cristina da; PEREIRA, Isabel Veloso Alves; NOGUEIRA, Marina Sayuri; YANGUAS, Sara Crespo; MAES, Michael; SILVA, Elisangela dos Anjos; DAGLI, Maria Lucia Zaidan; CASTRO, Inar Alves de; OLIVEIRA, Claudia Pinto; VINKEN, Mathieu; COGLIATI, Bruno |
Citation: | CLINICAL AND EXPERIMENTAL PHARMACOLOGY AND PHYSIOLOGY, v.44, n.2, p.197-206, 2017 |
Abstract: | Non-alcoholic steatohepatitis is a highly prevalent liver pathology featured by hepatocellular fat deposition and inflammation. Connexin32, which is the major building block of hepatocellular gap junctions, has a protective role in hepatocarcinogenesis and is downregulated in chronic liver diseases. However, the role of connexin32 in non-alcoholic steatohepatitis remains unclear. Connexin32(-/-) mice and their wild-type littermates were fed a choline-deficient high-fat diet. The manifestation of non-alcoholic steatohepatitis was evaluated based on a battery of clinically relevant read-outs, including histopathological examination, diverse indicators of inflammation and liver damage, in-depth lipid analysis, assessment of oxidative stress, insulin and glucose tolerance, liver regeneration and lipid-related biomarkers. Overall, more pronounced liver damage, inflammation and oxidative stress were observed in connexin32(-/-) mice compared to wild-type animals. No differences were found in insulin and glucose tolerance measurements and liver regeneration. However, two lipid-related genes, srebf1 and fabp3, were upregulated in Cx32(-/-) mice in comparison with wild-type animals. These findings suggest that connexin32-based signalling is not directly involved in steatosis as such, but rather in the sequelae of this process, which underlie progression of non-alcoholic steatohepatitis. |
Appears in Collections: | Artigos e Materiais de Revistas Científicas - FM/MGT Artigos e Materiais de Revistas Científicas - LIM/07 |
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