Sheep Model for Uterine Transplantation: The Best Option Before Starting a Human Program

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art_ANDRAUS_Sheep_Model_for_Uterine_Transplantation_The_Best_Option_2017.PDF (982.47 KB)
Citações na Scopus
20
Tipo de produção
article
Data de publicação
2017
DOI
SciELO
Scopus
Web of Science
PubMed
Título da Revista
ISSN da Revista
Título do Volume
Editora
HOSPITAL CLINICAS, UNIV SAO PAULO
Autores
Citação
CLINICS, v.72, n.3, p.178-182, 2017
Projetos de Pesquisa
Unidades Organizacionais
Fascículo
Resumo
OBJECTIVE: This study reports the first four cases of a uterine transplant procedure conducted in sheep in Latin America. The aim of this study was to evaluate the success of uterine transplantation in sheep. METHOD: The study was conducted at Laboratory of Medical Investigation 37 (LIM 37) at the University of Sao Paulo School of Medicine. Four healthy mature ewes weighing 40-60 kg were used as both the donor and recipient for a transplant within the same animal (auto-transplant). Institutional guidelines for the care of experimental animals were followed. RESULTS: The first two cases of auto-transplant were performed to standardize the technique. After complete uterine mobilization and isolation of the blood supply, the unilateral vascular pedicle was sectioned and anastomosed on the external iliac vessels. After standardization, the protocol was implemented. Procurement surgery was performed without complications or bleeding. After isolation of uterine arteries and veins as well as full mobilization of the uterus, ligation of the distal portion of the internal iliac vessels was performed with subsequent division and end-to-side anastomosis of the external iliac vessels. After vaginal anastomosis, the final case presented with arterial thrombosis in the left uterine artery. The left uterine artery anastomosis was re-opened and flushed with saline solution to remove the clot from the artery lumen. Anastomosis was repeated with restoration of blood flow for a few minutes before another uterine artery thrombosis appeared on the same side. All four animals were alive after the surgical procedure and were euthanized after the experimental period. CONCLUSION: We describe the success of four uterine auto-transplants in sheep models.
Palavras-chave
Uterine transplantation, Uterus, Transplantation, Sheep, Infertility
URI
https://observatorio.fm.usp.br/handle/OPI/20111
Referências
  1. Akar ME, 2015, J TURK-GER GYNECOL A, V16, P45, DOI 10.5152/jtgga.2015.15107
  2. Allahbadia GN, 2016, J OBSTET GYNECOL IND, V66, P1, DOI 10.1007/s13224-015-0805-7
  3. Avison DL, 2009, TRANSPLANTATION, V88, P465, DOI 10.1097/TP.0b013e3181b07666
  4. Brannstrom M, 2016, FERTIL STERIL, V106, P261, DOI 10.1016/j.fertnstert.2016.04.001
  5. Brannstrom M, 2015, CURR OPIN ORGAN TRAN, V20, P621, DOI 10.1097/MOT.0000000000000246
  6. Brannstrom M, 2015, LANCET, V385, P607, DOI 10.1016/S0140-6736(14)61728-1
  7. Brannstrom M, 2014, FERTIL STERIL, V101, P1228, DOI 10.1016/j.fertnstert.2014.02.024
  8. Brannstrom M, 2012, FERTIL STERIL, V97, P1269, DOI 10.1016/j.fertnstert.2012.04.001
  9. Dahm-Kahler P, 2008, J OBSTET GYNAECOL RE, V34, P784, DOI 10.1111/j.1447-0756.2008.00854.x
  10. Diaz-Garcia C, 2012, MOL HUM REPROD, V18, P68, DOI 10.1093/molehr/gar055
  11. Diaz-Garcia C, 2014, FERTIL STERIL, V102, P1545, DOI 10.1016/j.fertnstert.2014.09.010
  12. Diaz-Garcia C, 2010, ACTA OBSTET GYN SCAN, V89, P1491, DOI 10.3109/00016349.2010.520688
  13. El-Akouri RR, 2002, J ENDOCRINOL, V174, DOI 10.1677/joe.0.1740157
  14. Enskog A, 2010, HUM REPROD, V25, P1980, DOI 10.1093/humrep/deq109
  15. Gauthier T, 2011, HUM REPROD, V26, P3028, DOI 10.1093/humrep/der288
  16. Johannesson L, 2013, HUM REPROD, V28, P189, DOI 10.1093/humrep/des381
  17. Johannesson L, 2012, HUM REPROD, V27, P1640, DOI 10.1093/humrep/des093
  18. Johannesson L, 2015, FERTIL STERIL, V103, P199, DOI 10.1016/j.fertnstert.2014.09.024
  19. Kisu I, 2015, ACTA OBSTET GYN SCAN, V94, P942, DOI 10.1111/aogs.12701
  20. Milliez Jacques, 2009, Int J Gynaecol Obstet, V106, P270, DOI 10.1016/j.ijgo.2009.03.045
  21. PALERMO G, 1992, LANCET, V340, P17, DOI 10.1016/0140-6736(92)92425-F
  22. Racho El-Akouri R, 2003, HUM REPROD, V18, P2018
  23. Saso S, 2015, ACTA OBSTET GYN SCAN, V94, P245, DOI 10.1111/aogs.12550
  24. Sieunarine K, 2005, INT SURG, V90, P249
  25. STEPTOE PC, 1978, LANCET, V2, P366
  26. Wei L, 2013, PLOS ONE, V8, DOI 10.1371/journal.pone.0081300
  27. Wranning CA, 2010, HUM REPROD, V25, P1973, DOI 10.1093/humrep/deq130
  28. Wranning CA, 2006, J OBSTET GYNAECOL RE, V32, P358, DOI 10.1111/j.1447-0756.2006.00426.x

Coleções
Artigos e Materiais de Revistas Científicas - FM/MOG
Artigos e Materiais de Revistas Científicas - FM/MGT
Artigos e Materiais de Revistas Científicas - HC/ICHC
Artigos e Materiais de Revistas Científicas - LIM/37
Artigos e Materiais de Revistas Científicas - LIM/58