The interactions and essential effects of intrinsic insulin-like growth factor-I on Leishmania (Leishmania) major growth within macrophages

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dc.contributor Sistema FMUSP-HC: Faculdade de Medicina da Universidade de São Paulo (FMUSP) e Hospital das Clínicas da FMUSP
dc.contributor.author REIS, L. C. FMUSP-HC
RAMOS-SANCHEZ, E. M.
GOTO, H. FMUSP-HC
dc.date.issued 2013
dc.identifier.citation PARASITE IMMUNOLOGY, v.35, n.7-8, p.239-244, 2013
dc.identifier.issn 0141-9838
dc.identifier.uri http://observatorio.fm.usp.br/handle/OPI/2109
dc.description.abstract Previously, we showed in Leishmania infections that extrinsic insulin-like growth factor (IGF)-I favored Leishmania proliferation and leishmaniasis development. In this study, the interaction of intrinsically expressed IGF-I and Leishmania (Leishmania) major in macrophages was addressed, and a key finding was the observation, using confocal microscopy, of the co-localization of IGF-I and parasites within macrophages. Following stimulation with interferon-gamma (IFN-gamma), which is known to inhibit IGF-I production in macrophages, we observed a reduction in the expression of both IGF-I mRNA and protein. This reduced expression was accompanied by a reduction in the cellular parasite load that was completely recovered with the addition of extrinsic IGF-I, which suggests an essential role for IGF-I in Leishmania growth.
dc.description.sponsorship · Fundacao de Amparo a Pesquisa do Estado de Sao Paulo [FAPESP - 2008/04106-0]
· CNPq
· Hospital das Clinicas, Faculdade de Medicina, Universidade de Sao Paulo [FMUSP-HC/LIM-38]
dc.language.iso eng
dc.publisher WILEY-BLACKWELL
dc.relation.ispartof Parasite Immunology
dc.rights restrictedAccess
dc.subject confocal microscopy; insulin-like growth factor-I; Leishmania (Leishmania) major; macrophage; Interferon-gamma
dc.subject.other promoting factor; l-arginine; promastigotes; amastigotes; binding; activation; receptor
dc.title The interactions and essential effects of intrinsic insulin-like growth factor-I on Leishmania (Leishmania) major growth within macrophages
dc.type article
dc.rights.holder Copyright WILEY-BLACKWELL
dc.description.group LIM/38
dc.identifier.doi 10.1111/pim.12041
dc.identifier.pmid 23668415
dc.type.category original article
dc.type.version publishedVersion
hcfmusp.author REIS, L. C.:HC:INCOR
hcfmusp.author GOTO, H.:FM:MPR
hcfmusp.author.external · RAMOS-SANCHEZ, E. M.:Univ Sao Paulo, IMTSP, BR-05403000 Sao Paulo, Brazil
hcfmusp.origem.id 2-s2.0-84879806025
hcfmusp.origem.id WOS:000321188100006
hcfmusp.publisher.city HOBOKEN
hcfmusp.publisher.country USA
hcfmusp.relation.reference · Al-Salam S, 2009, ISLETS, V1, P102, DOI 10.4161/isl.1.2.9273
· ARKINS S, 1995, MOL ENDOCRINOL, V9, P350, DOI 10.1210/me.9.3.350
· ARKINS S, 1993, ENDOCRINOLOGY, V133, P2334, DOI 10.1210/en.133.5.2334
· Contreras I, 2010, PLOS PATHOG, V6
· Costa FAL, 2000, BRAZ J MED BIOL RES, V33, P1455, DOI 10.1590/S0100-879X2000001200009
· Desjeux P, 2004, COMP IMMUNOL MICROB, V27, P305, DOI 10.1016/j.cimid.2004.03.004
· Gomes CMC, 2001, EXP PARASITOL, V99, P190, DOI 10.1006/expr.2001.4650
· Gomes CMC, 1998, J EUKARYOT MICROBIOL, V45, P352, DOI 10.1111/j.1550-7408.1998.tb04548.x
· Gomes CMC, 1997, ACTA TROP, V64, P225, DOI 10.1016/S0001-706X(96)00633-X
· Gomes CMD, 2000, INT J EXP PATHOL, V81, P249, DOI 10.1046/j.1365-2613.2000.00157.x
· Goto H, 1998, P NATL ACAD SCI USA, V95, P13211, DOI 10.1073/pnas.95.22.13211
· GREEN LC, 1982, ANAL BIOCHEM, V126, P131, DOI 10.1016/0003-2697(82)90118-X
· GREEN SJ, 1990, J IMMUNOL, V144, P278
· Haider HK, 2008, CIRC RES, V103, P1300, DOI 10.1161/CIRCRESAHA.108.186742
· Hewitt SC, 2010, J BIOL CHEM, V285, P2676, DOI 10.1074/jbc.M109.043471
· JONES JI, 1995, ENDOCR REV, V16, P3, DOI 10.1210/er.16.1.3
· Kihira T, 2009, NEUROPATHOLOGY, V29, P548, DOI 10.1111/j.1440-1789.2009.01010.x
· LIEW FY, 1990, J IMMUNOL, V144, P4794
· Mougneau E, 2011, IMMUNOL REV, V240, P286, DOI 10.1111/j.1600-065X.2010.00983.x
· Pfaffl MW, 2001, NUCLEIC ACIDS RES, V29, P2002, DOI 10.1093/NAR/29.9.E45
· Roberts SC, 2004, J BIOL CHEM, V279, P23668, DOI 10.1074/jbc.M402042200
· Vendrame CMV, 2007, SCAND J IMMUNOL, V66, P287, DOI 10.1111/j.1365-3083.2007.01950.x
· Verschure PJ, 1996, HISTOCHEM J, V28, P13, DOI 10.1007/BF02331423
· Wynes MW, 2003, J IMMUNOL, V171, P3550
dc.description.index MEDLINE
hcfmusp.citation.scopus 10
hcfmusp.citation.wos 10
hcfmusp.affiliation.country Brasil


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