The interactions and essential effects of intrinsic insulin-like growth factor-I on Leishmania (Leishmania) major growth within macrophages

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dc.contributor Sistema FMUSP-HC: Faculdade de Medicina da Universidade de São Paulo (FMUSP) e Hospital das Clínicas da FMUSP REIS, L. C. FMUSP-HC
dc.identifier.citation PARASITE IMMUNOLOGY, v.35, n.7-8, p.239-244, 2013
dc.identifier.issn 0141-9838
dc.description.abstract Previously, we showed in Leishmania infections that extrinsic insulin-like growth factor (IGF)-I favored Leishmania proliferation and leishmaniasis development. In this study, the interaction of intrinsically expressed IGF-I and Leishmania (Leishmania) major in macrophages was addressed, and a key finding was the observation, using confocal microscopy, of the co-localization of IGF-I and parasites within macrophages. Following stimulation with interferon-gamma (IFN-gamma), which is known to inhibit IGF-I production in macrophages, we observed a reduction in the expression of both IGF-I mRNA and protein. This reduced expression was accompanied by a reduction in the cellular parasite load that was completely recovered with the addition of extrinsic IGF-I, which suggests an essential role for IGF-I in Leishmania growth.
dc.description.sponsorship · Fundacao de Amparo a Pesquisa do Estado de Sao Paulo [FAPESP - 2008/04106-0]
· CNPq
· Hospital das Clinicas, Faculdade de Medicina, Universidade de Sao Paulo [FMUSP-HC/LIM-38]
dc.language.iso eng
dc.publisher WILEY-BLACKWELL
dc.relation.ispartof Parasite Immunology
dc.rights restrictedAccess
dc.subject confocal microscopy; insulin-like growth factor-I; Leishmania (Leishmania) major; macrophage; Interferon-gamma
dc.subject.other promoting factor; l-arginine; promastigotes; amastigotes; binding; activation; receptor
dc.title The interactions and essential effects of intrinsic insulin-like growth factor-I on Leishmania (Leishmania) major growth within macrophages
dc.type article
dc.rights.holder Copyright WILEY-BLACKWELL LIM/38
dc.identifier.doi 10.1111/pim.12041
dc.identifier.pmid 23668415
dc.type.category original article
dc.type.version publishedVersion REIS, L. C.:HC:INCOR GOTO, H.:FM:MPR · RAMOS-SANCHEZ, E. M.:Univ Sao Paulo, IMTSP, BR-05403000 Sao Paulo, Brazil 2-s2.0-84879806025 WOS:000321188100006 HOBOKEN USA
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dc.description.index MEDLINE
hcfmusp.citation.scopus 10
hcfmusp.citation.wos 10 Brasil

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