New evidences on the regulation of SF-1 expression by POD1/TCF21 in adrenocortical tumor cells

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dc.contributor Sistema FMUSP-HC: Faculdade de Medicina da Universidade de São Paulo (FMUSP) e Hospital das Clínicas da FMUSP
dc.contributor.author FRANCA, Monica Malheiros
LERARIO, Antonio M. FMUSP-HC
FRAGOSO, Maria Candida B. V. FMUSP-HC
LOTFI, Claudimara Ferini Pacicco
dc.date.issued 2017
dc.identifier.citation CLINICS, v.72, n.6, p.391-394, 2017
dc.identifier.issn 1807-5932
dc.identifier.uri http://observatorio.fm.usp.br/handle/OPI/21472
dc.description.abstract OBJECTIVES: Transcription Factor 21 represses steroidogenic factor 1, a nuclear receptor required for gonadal development, sex determination and the regulation of adrenogonadal steroidogenesis. The aim of this study was to investigate whether silencing or overexpression of the gene Transcription Factor 21 could modulate the gene and protein expression of steroidogenic factor 1 in adrenocortical tumors. METHODS: We analyzed the gene expression of steroidogenic factor 1 using qPCR after silencing endogenous Transcription Factor 21 in pediatric adrenal adenoma-T7 cells through small interfering RNA. In addition, using overexpression of Transcription Factor 21 in human adrenocortical carcinoma cells, we analyzed the protein expression of steroidogenic factor 1 using Western blotting. RESULTS: Transcription Factor 21 knockdown increased the mRNA expression of steroidogenic factor 1 by 5.97-fold in pediatric adrenal adenoma-T7 cells. Additionally, Transcription Factor 21 overexpression inhibited the protein expression of steroidogenic factor 1 by 0.41-fold and 0.64-fold in two different adult adrenocortical carcinoma cell cultures, H295R and T36, respectively. CONCLUSIONS: Transcription Factor 21 is downregulated in adrenocortical carcinoma cells. Taken together, these findings support the hypothesis that Transcription Factor 21 is a regulator of steroidogenic factor 1 and is a tumor suppressor gene in pediatric and adult adrenocortical tumors.
dc.description.sponsorship · Sao Paulo Research Foundation (FAPESP) [2010/00771-9]
· FAPESP [2012/21839-6]
· National Council for Scientific and Technological Development (CNPq)
· Office of the Dean for Research at the University of Sao Paulo
dc.language.iso eng
dc.publisher HOSPITAL CLINICAS, UNIV SAO PAULO
dc.relation.ispartof Clinics
dc.rights openAccess
dc.subject TCF21; POD1; SF-1; siRNA-POD1; Adrenocortical Tumor Cells
dc.subject.other steroidogenic factor-i; star expression; gene; overexpression; mesenchyme; kidney; cancer; tcf21; pod-1; lung
dc.title New evidences on the regulation of SF-1 expression by POD1/TCF21 in adrenocortical tumor cells
dc.type article
dc.rights.holder Copyright HOSPITAL CLINICAS, UNIV SAO PAULO
dc.description.group LIM/42
dc.identifier.doi 10.6061/clinics/2017(06)10
dc.identifier.pmid 28658440
dc.type.category original article
dc.type.version publishedVersion
hcfmusp.author LERARIO, Antonio M.:HC:ICESP
hcfmusp.author FRAGOSO, Maria Candida B. V.:HC:ICHC
hcfmusp.author.external · FRANCA, Monica Malheiros:Univ Sao Paulo, Inst Ciencias Biomed, Dept Anat, Sao Paulo, SP, Brazil
· LOTFI, Claudimara Ferini Pacicco:Univ Sao Paulo, Inst Ciencias Biomed, Dept Anat, Sao Paulo, SP, Brazil
hcfmusp.origem.id 2-s2.0-85021230518
hcfmusp.origem.id WOS:000404479600010
hcfmusp.origem.id SCIELO:S1807-59322017000600391
hcfmusp.publisher.city SAO PAULO
hcfmusp.publisher.country BRAZIL
hcfmusp.relation.reference · Almeida MQ, 2008, J CLIN ENDOCR METAB, V93, P3524, DOI 10.1210/jc.2008-0065
· Arab K, 2011, CARCINOGENESIS, V32, P1467, DOI 10.1093/carcin/bgr138
· Beuschlein F, 2002, ENDOCRINOLOGY, V143, P3122, DOI 10.1210/en.143.8.3122
· Doghman M, 2007, MOL ENDOCRINOL, V21, P2968, DOI 10.1210/me.2007-0120
· Figueiredo BC, 2005, J CLIN ENDOCR METAB, V90, P615, DOI 10.1210/jc.2004-0942
· Franca MM, 2015, BRAZ J MED BIOL RES, V48, P1087, DOI 10.1590/1414-431X20154748
· Franca MM, 2015, BIOMED RES INT, DOI 10.1155/2015/841784
· Franca MM, 2013, MOL CELL ENDOCRINOL, V371, P140, DOI 10.1016/j.mce.2012.12.029
· GAZDAR AF, 1990, CANCER RES, V50, P5488
· Giordano TJ, 2009, CLIN CANCER RES, V15, P668, DOI 10.1158/1078-0432.CCR-08-1067
· Kim AC, 2009, ENDOCR REV, V30, P241, DOI 10.1210/er.2008-0039
· Livak KJ, 2001, METHODS, V25, P402, DOI 10.1006/meth.2001.1262
· Lu JR, 1998, MECH DEVELOP, V73, P23, DOI 10.1016/S0925-4773(98)00030-6
· Pianovski MAD, 2006, EUR J CANCER, V42, P1040, DOI 10.1016/j.ejca.2006.01.022
· Quaggin SE, 1998, MECH DEVELOP, V71, P37, DOI 10.1016/S0925-4773(97)00201-3
· Robb L, 1998, DEV DYNAM, V213, P105, DOI 10.1002/(SICI)1097-0177(199809)213:1<105::AID-AJA10>3.0.CO;2-1
· Sbiera S, 2010, J CLIN ENDOCR METAB, V95, pE161, DOI 10.1210/jc.2010-0653
· Smith LT, 2006, P NATL ACAD SCI USA, V103, P982, DOI 10.1073/pnas.0510171102
· Tamura M, 2001, MECH DEVELOP, V102, P135, DOI 10.1016/S0925-4773(01)00298-2
dc.description.index MEDLINE
dc.identifier.eissn 1980-5322
hcfmusp.citation.scopus 0
hcfmusp.citation.wos 1
hcfmusp.affiliation.country Brasil


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