New evidences on the regulation of SF-1 expression by POD1/TCF21 in adrenocortical tumor cells

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dc.contributor Sistema FMUSP-HC: Faculdade de Medicina da Universidade de São Paulo (FMUSP) e Hospital das Clínicas da FMUSP FRANCA, Monica Malheiros
FRAGOSO, Maria Candida B. V. FMUSP-HC
LOTFI, Claudimara Ferini Pacicco 2017
dc.identifier.citation CLINICS, v.72, n.6, p.391-394, 2017
dc.identifier.issn 1807-5932
dc.description.abstract OBJECTIVES: Transcription Factor 21 represses steroidogenic factor 1, a nuclear receptor required for gonadal development, sex determination and the regulation of adrenogonadal steroidogenesis. The aim of this study was to investigate whether silencing or overexpression of the gene Transcription Factor 21 could modulate the gene and protein expression of steroidogenic factor 1 in adrenocortical tumors. METHODS: We analyzed the gene expression of steroidogenic factor 1 using qPCR after silencing endogenous Transcription Factor 21 in pediatric adrenal adenoma-T7 cells through small interfering RNA. In addition, using overexpression of Transcription Factor 21 in human adrenocortical carcinoma cells, we analyzed the protein expression of steroidogenic factor 1 using Western blotting. RESULTS: Transcription Factor 21 knockdown increased the mRNA expression of steroidogenic factor 1 by 5.97-fold in pediatric adrenal adenoma-T7 cells. Additionally, Transcription Factor 21 overexpression inhibited the protein expression of steroidogenic factor 1 by 0.41-fold and 0.64-fold in two different adult adrenocortical carcinoma cell cultures, H295R and T36, respectively. CONCLUSIONS: Transcription Factor 21 is downregulated in adrenocortical carcinoma cells. Taken together, these findings support the hypothesis that Transcription Factor 21 is a regulator of steroidogenic factor 1 and is a tumor suppressor gene in pediatric and adult adrenocortical tumors.
dc.description.sponsorship · Sao Paulo Research Foundation (FAPESP) [2010/00771-9]
· FAPESP [2012/21839-6]
· National Council for Scientific and Technological Development (CNPq)
· Office of the Dean for Research at the University of Sao Paulo
dc.language.iso eng
dc.relation.ispartof Clinics
dc.rights openAccess
dc.subject TCF21; POD1; SF-1; siRNA-POD1; Adrenocortical Tumor Cells
dc.subject.other steroidogenic factor-i; star expression; gene; overexpression; mesenchyme; kidney; cancer; tcf21; pod-1; lung
dc.title New evidences on the regulation of SF-1 expression by POD1/TCF21 in adrenocortical tumor cells
dc.type article
dc.rights.holder Copyright HOSPITAL CLINICAS, UNIV SAO PAULO LIM/42
dc.identifier.doi 10.6061/clinics/2017(06)10
dc.identifier.pmid 28658440
dc.type.category original article
dc.type.version publishedVersion LERARIO, Antonio M.:HC:ICESP FRAGOSO, Maria Candida B. V.:HC:ICHC · FRANCA, Monica Malheiros:Univ Sao Paulo, Inst Ciencias Biomed, Dept Anat, Sao Paulo, SP, Brazil
· LOTFI, Claudimara Ferini Pacicco:Univ Sao Paulo, Inst Ciencias Biomed, Dept Anat, Sao Paulo, SP, Brazil 2-s2.0-85021230518 WOS:000404479600010 SCIELO:S1807-59322017000600391 SAO PAULO BRAZIL
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dc.description.index MEDLINE
dc.identifier.eissn 1980-5322
hcfmusp.citation.scopus 0
hcfmusp.citation.wos 1 Brasil

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