Different Assay Conditions for Detecting the Production and Release of Heat-Labile and Heat-Stable Toxins in Enterotoxigenic Escherichia coli Isolates

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dc.contributor Sistema FMUSP-HC: Faculdade de Medicina da Universidade de São Paulo (FMUSP) e Hospital das Clínicas da FMUSP
dc.contributor.author ROCHA, Leticia B.
OZAKI, Christiane Y. FMUSP-HC
HORTON, Denise S. P. Q.
MENEZES, Caroline A.
SILVA, Anderson
FERNANDES, Irene
MAGNOLI, Fabio C.
VAZ, Tania M. I.
GUTH, Beatriz E. C.
PIAZZA, Roxane M. F.
dc.date.issued 2013
dc.description.abstract Enterotoxigenic Escherichia coli (ETEC) produce heat-labile (LT) and/or heat-stable enterotoxins (ST). Despite that, the mechanism of action of both toxins are well known, there is great controversy in the literature concerning the in vitro production and release of LT and, for ST, no major concerns have been discussed. Furthermore, the majority of published papers describe the use of only one or a few ETEC isolates to define the production and release of these toxins, which hinders the detection of ETEC by phenotypic approaches. Thus, the present study was undertaken to obtain a better understanding of ST and LT toxin production and release under laboratory conditions. Accordingly, a collection of 90 LT-, ST-, and ST/LT-producing ETEC isolates was used to determine a protocol for toxin production and release aimed at ETEC detection. For this, we used previously raised anti-LT antibodies and the anti-ST monoclonal and polyclonal antibodies described herein. The presence of bile salts and the use of certain antibiotics improved ETEC toxin production/release. Triton X-100, as chemical treatment, proved to be an alternative method for toxin release. Consequently, a common protocol that can increase the production and release of LT and ST toxins could facilitate and enhance the sensitivity of diagnostic tests for ETEC using the raised and described antibodies in the present work.
dc.description.group LIM/38
dc.description.index MEDLINE
dc.description.sponsorship · Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP)
· Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq)
· FAPESP
dc.identifier.citation TOXINS, v.5, n.12, p.2384-2402, 2013
dc.identifier.doi 10.3390/toxins5122384
dc.identifier.issn 2072-6651
dc.identifier.pmid 24316604
dc.identifier.uri http://observatorio.fm.usp.br/handle/OPI/21621
dc.language.iso eng
dc.publisher MDPI AG
dc.relation.ispartof Toxins
dc.rights openAccess
dc.rights.holder Copyright MDPI AG
dc.subject ETEC; heat-labile toxin; heat-stable toxin; production; release; detection
dc.subject.other colonization factor antigens; entero-toxin; cholera-toxin; outer-membrane; immunosorbent-assay; vibrio-cholerae; sao-paulo; strains; protein; diarrhea
dc.title Different Assay Conditions for Detecting the Production and Release of Heat-Labile and Heat-Stable Toxins in Enterotoxigenic Escherichia coli Isolates
dc.type article
dc.type.category original article
dc.type.version publishedVersion
hcfmusp.author OZAKI, Christiane Y.:IMT:SCSORO-83
hcfmusp.author.external · ROCHA, Leticia B.:Butantan Inst, Bacteriol Lab, BR-05503900 Sao Paulo, Brazil
· HORTON, Denise S. P. Q.:Butantan Inst, Bacteriol Lab, BR-05503900 Sao Paulo, Brazil
· MENEZES, Caroline A.:Butantan Inst, Bacteriol Lab, BR-05503900 Sao Paulo, Brazil; Fleury Med & Hlth, BR-04344903 Sao Paulo, Brazil
· SILVA, Anderson:Butantan Inst, Bacteriol Lab, BR-05503900 Sao Paulo, Brazil
· FERNANDES, Irene:Butantan Inst, Immunopathol Lab, BR-05503900 Sao Paulo, Brazil
· MAGNOLI, Fabio C.:Butantan Inst, Immunochem Lab, BR-05503900 Sao Paulo, Brazil
· VAZ, Tania M. I.:Adolfo Lutz Inst, Bacteriol Sect, BR-01246000 Sao Paulo, Brazil
· GUTH, Beatriz E. C.:Univ Fed Sao Paulo, Escola Paulista Med, Dept Microbiol, BR-04923062 Sao Paulo, SP, Brazil
· PIAZZA, Roxane M. F.:Butantan Inst, Bacteriol Lab, BR-05503900 Sao Paulo, Brazil
hcfmusp.origem.id WOS:000332792300007
hcfmusp.publisher.city BASEL
hcfmusp.publisher.country SWITZERLAND
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