Transcriptomic analysis of purified human cortical microglia reveals age-associated changes

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art_GALATRO_Transcriptomic_analysis_of_purified_human_cortical_microglia_reveals_2017.PDF (4.72 MB)
Citações na Scopus
476
Tipo de produção
article
Data de publicação
2017
Editora
NATURE PUBLISHING GROUP
DOI
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Scopus
Web of Science
PubMed
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ISSN da Revista
Título do Volume
Autores
HOLTMAN, Inge R.
LERARIO, Antonio M.
VAINCHTEIN, Ilia D.
BROUWER, Nieske
PEREIRA, Tulio F.
MOLLER, Thomas
Autor de Grupo de pesquisa
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Citação
NATURE NEUROSCIENCE, v.20, n.8, p.1162-0, 2017
Projetos de Pesquisa
Unidades Organizacionais
Fascículo
Resumo
Microglia are essential for CNS homeostasis and innate neuroimmune function, and play important roles in neurodegeneration and brain aging. Here we present gene expression profiles of purified microglia isolated at autopsy from the parietal cortex of 39 human subjects with intact cognition. Overall, genes expressed by human microglia were similar to those in mouse, including established microglial genes CX3CR1, P2RY12 and ITGAM (CD11B). However, a number of immune genes, not identified as part of the mouse microglial signature, were abundantly expressed in human microglia, including TLR, F-c gamma and SIGLEC receptors, as well as TAL1 and IFI16, regulators of proliferation and cell cycle. Age-associated changes in human microglia were enriched for genes involved in cell adhesion, axonal guidance, cell surface receptor expression and actin (dis)assembly. Limited overlap was observed in microglial genes regulated during aging between mice and humans, indicating that human and mouse microglia age differently.
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URI
https://observatorio.fm.usp.br/handle/OPI/22014
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Coleções
Artigos e Materiais de Revistas Científicas - FM/MPT
Artigos e Materiais de Revistas Científicas - FM/MNE
Artigos e Materiais de Revistas Científicas - LIM/05
Artigos e Materiais de Revistas Científicas - LIM/15
Artigos e Materiais de Revistas Científicas - LIM/22
Artigos e Materiais de Revistas Científicas - ODS/03