Basal core promoter and precore mutations among hepatitis B virus circulating in Brazil and its association with severe forms of hepatic diseases
Carregando...
Citações na Scopus
10
Tipo de produção
article
Data de publicação
2017
Editora
FUNDACO OSWALDO CRUZ
Indexadores
Título da Revista
ISSN da Revista
Título do Volume
Autores
CHACHA, Silvana Gama Florencio
FERREIRA, Sandro da Costa
VILLANOVA, Mrcia Guimaraes
SOUZA, Fernanda Fernandes
TEIXEIRA, Andreza Correa
PASSOS, Afonso Dinis da Costa
MARTINELLI, Ana de Lourdes Candolo
Autor de Grupo de pesquisa
Editores
Coordenadores
Organizadores
Citação
MEMORIAS DO INSTITUTO OSWALDO CRUZ, v.112, n.9, p.626-631, 2017
Resumo
BACKGROUND In Brazil, few studies have investigated the prevalence of infection with the precore (PC) and basal core promoter (BCP) mutants of the hepatitis B virus (HBV). OBJECTIVES This study aimed to analyse the frequency of PC and BCP mutations among patients infected with HBV and to evaluate the association between the variants and advanced hepatic disease. METHODS A total of 161 patients infected with HBV were studied. To identify PC and BCP mutations, a 501-bp fragment of HBV DNA was amplified and sequenced. FINDINGS PC and BCP regions from HBV strains were successfully amplified and sequenced in 129 and 118 cases, respectively. PC and BCP mutations were detected in 61.0% and 80.6% of the cases, respectively. The A1762T/G1764A variant was identified in 36.7% of the patients with grade 1 and 2 liver fibrosis (29/79) and in 81.8% of the patients with grade 3 and 4 liver fibrosis (9/11) (p < 0.01); in 76.9% of the patients with cirrhosis (10/13) and in 38.1% of the patients without cirrhosis (40/105) (p = 0.01); and in 77.8% of the patients with hepatocellular carcinoma (HCC) (7/9) and in 39.4% of the patients without HCC (43/109) (p = 0.03). MAIN CONCLUSIONS A high prevalence of HBV PC and BCP mutants was found. The A1762T/G1764A variant was independently associated with advanced forms of liver fibrosis, hepatic cirrhosis, and HCC.
Palavras-chave
hepatitis B virus, basal core promoter mutant, precore mutant, Brazil
Referências
- Asim M, 2010, J MED VIROL, V82, P1115, DOI 10.1002/jmv.21774
- Bosch J, 2008, SEMIN LIVER DIS, V28, P3, DOI 10.1055/s-2008-1040318
- Bruix J, 2005, HEPATOLOGY, V42, P1208, DOI 10.1002/hep20933
- Chacha SGF, 2017, BRAZ J INFECT DIS
- Chang KM, 2010, CLIN LIVER DIS, V14, P409, DOI 10.1016/j.cld.2010.05.012
- Dandri M, 2012, GUT, V61, P6, DOI 10.1136/gutjnl-2012-302056
- DESMET VJ, 1994, HEPATOLOGY, V19, P1513, DOI 10.1016/0270-9139(94)90250-X
- Gomes-Gouvea MS, 2015, ANTIVIR THER, V20, P387, DOI 10.3851/IMP2938
- Hadziyannis SJ, 2001, HEPATOLOGY, V34, P617, DOI 10.1053/jhep.2001.27834
- Jammeh S, 2008, J GEN VIROL, V89, P901, DOI 10.1099/vir.0.83468-0
- Kao JH, 2003, GASTROENTEROLOGY, V124, P327, DOI 10.1053/gast.2003.50053
- KNODELL RG, 1981, HEPATOLOGY, V1, P431, DOI 10.1002/hep.1840010511
- Kramvis A, 1998, J HEPATOL, V28, P132, DOI 10.1016/S0168-8278(98)80212-2
- Laras A, 1998, J VIRAL HEPATITIS, V5, P241, DOI 10.1046/j.1365-2893.1998.00109.x
- LI JS, 1993, J VIROL, V67, P5402
- Liu CJ, 2011, J VIRAL HEPATITIS, V18, pE482, DOI 10.1111/j.1365-2893.2011.01480.x
- Llovet Josep M, 2004, Liver Transpl, V10, pS115, DOI 10.1002/lt.20034
- Look ASF, 2009, HEPATOLOGY, V50, P1
- McMahon BJ, 2010, CLIN LIVER DIS, V14, P381, DOI 10.1016/j.cld.2010.05.007
- Puche ML, 2016, INVEST CLIN, V57, P38
- Sitnik R, 2010, REV INST MED TROP SP, V52, P119, DOI [10.1590/50036-46652010000300001, 10.1590/S0036-46652010000300001]
- Yang HI, 2008, J NATL CANCER I, V100, P1134, DOI 10.1093/jnci/djn243
- Yang ZG, 2016, ONCOTARGET, V7, P12525, DOI 10.18632/oncotarget.7123
- Zheng JX, 2011, J VIRAL HEPATITIS, V18, pE423, DOI 10.1111/j.1365-2893.2011.01458.x