Detection of Leishmania (Leishmania) infantum RNA in fleas and ticks collected from naturally infected dogs
Carregando...
Citações na Scopus
53
Tipo de produção
article
Data de publicação
2011
Editora
SPRINGER
Indexadores
Título da Revista
ISSN da Revista
Título do Volume
Autores
COLOMBO, Fabio A.
ODORIZZI, Rosa M. F. N.
GALATI, Eunice A. B.
CANAVEZ, Flavio
PEREIRA-CHIOCCOLA, Vera L.
Autor de Grupo de pesquisa
Editores
Coordenadores
Organizadores
Citação
PARASITOLOGY RESEARCH, v.109, n.2, p.267-274, 2011
Resumo
The occurrence of the insect vector (sand flies) with low rates of Leishmania infection, as well as autochthonous transmission in the absence of the natural vector in dogs, have been reported. These unexpected data suggest a hypothesis of other arthropods as a possible way of Leishmania transmission. The prevalence of Leishmania (Leishmania) infantum in fleas and ticks collected from dogs with canine visceral leishmaniasis (CVL), as well as parasite viability, were evaluated herein. The presence of L. (L.) infantum was assayed by PCR and ELISA in ectoparasites and biological samples from 73 dogs living in a Brazilian endemic area. As the occurrence of Leishmania DNA in ticks and fleas is expected given their blood-feeding habits, we next investigated whether parasites can remain viable inside ticks. PCR and ELISA confirmed that 83% of the dogs had CVL. Fleas and ticks (nymphs, male and female adults) were collected in 55% and 63% of the 73 dogs, respectively. Out of the 60 dogs with CVL, 80% harbored ectoparasites infected with L. (L.) infantum. The infection rates of the ectoparasites were 23% and 50% for fleas and ticks, respectively. The RNA analysis of the extract from ticks left in laboratory conditions during 7 to 10 days after removal from CVL dogs showed that parasites were alive. In addition, live parasites were also detected inside adult ticks recently molted in laboratory conditions. These findings indicate a higher infection rate of L. (L.) infantum in ticks and fleas, but they do not conclusively demonstrate whether these ticks can act as vectors of CVL, despite the fact that their rates were higher than those previously described in Lutzomyia longipalpis. The presence of viable L. (L.) infantum in ticks suggests the possible importance of dog ectoparasites in CVL dissemination.
Palavras-chave
Referências
- Dantas-Torres F, 2006, REV SAUDE PUBL, V40, P537, DOI [10.1590/S0034-89102006000300024, 10.1590/s0034-89102006000300024]
- Rosypal AC, 2005, J PARASITOL, V91, P970, DOI 10.1645/GE-483R.1
- Michalsky EM, 2009, MEM I OSWALDO CRUZ, V104, P1191, DOI 10.1590/S0074-02762009000800020
- Duprey ZH, 2006, EMERG INFECT DIS, V12, P440
- Coutinho MTZ, 2005, VET PARASITOL, V128, P149, DOI 10.1016/j.vetpar.2004.11.011
- Ferreira MGPA, 2009, VET PARASITOL, V165, P150, DOI 10.1016/j.vetpar.2009.06.026
- Coutinho MTZ, 2007, VET PARASITOL, V147, P320, DOI 10.1016/j.vetpar.2007.04.008
- Dantas-Torres F, 2007, VET PARASITOL, V149, P139, DOI 10.1016/j.vetpar.2007.07.007
- Lainson R, 2005, MEM I OSWALDO CRUZ, V100, P811, DOI 10.1590/S0074-02762005000800001
- Savani ESMM, 2009, VET PARASITOL, V160, P18, DOI 10.1016/j.vetpar.2008.10.090
- Baneth G, 2008, TRENDS PARASITOL, V24, P324, DOI 10.1016/j.pt.2008.04.001
- Troughton DR, 2007, J MED ENTOMOL, V44, P732, DOI 10.1603/0022-2585(2007)44[732:LCOSIT]2.0.CO;2
- Gomes AHS, 2007, VET PARASITOL, V144, P234, DOI 10.1016/j.vetpar.2006.10.008
- Stuart K, 2008, J CLIN INVEST, V118, P1301, DOI 10.1172/JCI33945
- Schantz PM, 2005, JAVMA-J AM VET MED A, V226, P1316, DOI 10.2460/javma.2005.226.1316
- le Fichoux Y, 1999, J CLIN MICROBIOL, V37, P1953
- Kullberg M, 2006, MOL BIOL EVOL, V23, P1493, DOI 10.1093/molbev/msl027
- Aagaard-Hansen J, 2010, TROP MED INT HEALTH, V15, P1281, DOI 10.1111/j.1365-3156.2010.02629.x
- GRIMALDI G, 1993, CLIN MICROBIOL REV, V6, P230
- Missawa NA, 2007, MEM I OSWALDO CRUZ, V102, P913, DOI 10.1590/S0074-02762007000800004
- Dantas-Torres F, 2010, PARASITOL RES, V106, P857, DOI 10.1007/s00436-010-1722-4
- DEGRAVE W, 1994, MEM I OSWALDO CRUZ, V89, P463
- Lachaud L, 2002, J CLIN MICROBIOL, V40, P210, DOI 10.1128/JCM.40.1.210-215.2002
- Silva FL, 2009, VET PARASITOL, V160, P55, DOI 10.1016/j.vetpar.2008.10.079
- Dantas-Torres F, 2010, EXP PARASITOL, V125, P184, DOI 10.1016/j.exppara.2010.02.003
- Aragao H.de B., 1936, Memorias do Instituto Oswaldo Cruz, V31, P759
- CAMARGONEVES VLF, 2004, B EPID PAUL, V6
- Blanc G, 1930, CR HEBD ACAD SCI, V191, P1162
- Costa C H, 2001, Rev Soc Bras Med Trop, V34, P223
- Dantas-Torres F, 2005, REV SOC BRAS MED TRO, V38, P444, DOI 10.1590/S0037-86822005000500017
- DiazEspineira MM, 1997, VET QUART, V19, P69
- FREITAS E., 2006, VET PARASITOL, V137, P159
- Gaskin AA, 2002, J VET INTERN MED, V16, P34, DOI 10.1892/0891-6640(2002)016<0034:VLIANY>2.3.CO;2
- GIRAUD P, 1954, Arch Fr Pediatr, V11, P337
- LAINSON R, 1998, TOPLEY WILSONS MICRO, V5
- LINARI PM, 2000, SIFONAPTEROS BRASIL
- MSB-Ministerio da Saude do Brasil (Health Ministry of Brazil), 2010, MAN VIG CONTR LEISHM
- Odorizzi RMFN, 2007, REV SAUDE PUBL, V41, P645, DOI [10.1590/S0034-89102007000400020, 10.1590/s0034-89102007000400020]
- PAZ GF, 2010, PARASITOL RES, V6, P523
- Pereira-Chioccola V.L., 2009, BOL EPIDEMIOL PAUL, V6, P4
- RAVEL S, 1995, ACTA TROP, V59, P187, DOI 10.1016/0001-706X(95)00079-T
- SHERLOCK I A, 1964, Rev Bras Malariol Doencas Trop, V16, P19
- SILVA SM, 2009, VET PARASITOL, V3, P159