MicroRNAs 29 are involved in the improvement of ventricular compliance promoted by aerobic exercise training in rats

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art_SOCI_MicroRNAs_29_are_involved_in_the_improvement_of_2011.PDF (629.13 KB)
Citações na Scopus
156
Tipo de produção
article
Data de publicação
2011
Editora
AMER PHYSIOLOGICAL SOC
DOI
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Scopus
Web of Science
PubMed
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Título do Volume
Autores
SOCI, U. P. R.
FERNANDES, T.
HASHIMOTO, N. Y.
MOTA, G. F.
AMADEU, M. A.
ROSA, K. T.
PHILLIPS, M. I.
OLIVEIRA, E. M.
Autor de Grupo de pesquisa
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Coordenadores
Organizadores
Citação
PHYSIOLOGICAL GENOMICS, v.43, n.11, p.665-673, 2011
Projetos de Pesquisa
Unidades Organizacionais
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Resumo
Soci UPR, Fernandes T, Hashimoto NY, Mota GF, Amadeu MA, Rosa KT, Irigoyen MC, Phillips MI, Oliveira EM. MicroRNAs 29 are involved in the improvement of ventricular compliance promoted by aerobic exercise training in rats. Physiol Genomics 43: 665-673, 2011. First published March 29, 2011; doi:10.1152/physiolgenomics.00145.2010.-MiRNAs regulate cardiac development, hypertrophy, and angiogenesis, but their role in cardiac hypertrophy (CH) induced by aerobic training has not previously been studied. Aerobic training promotes physiological CH preserving cardiac function. This study assessed involvement of miRNAs-29 in CH of trained rats. Female Wistar rats (n = 7/group) were randomized into three groups: sedentary (S), training 1 (T1), training 2 (T2). T1: swimming sessions of 60 min/5 days/wk/10 wk. T2: similar to T1 until 8th wk. On the 9th wk rats swam 2x/day, and on the 10th wk 3x/day. MiRNAs analysis was performed by miRNA microarray and confirmed by real-time PCR. We assessed: markers of training, CH by ratio of left ventricle (LV) weight/body wt and cardiomyocytes diameter, pathological markers of CH (ANF, skeletal alpha-actin, alpha/beta-MHC), collagen I and III (COLIAI and COLIIIAI) by real-time PCR, protein collagen by hydroxyproline (OH-proline) concentration, CF and CH by echocardiography. Training improved aerobic capacity and induced CH. MiRNAs-1, 133a, and 133b were downregulated as observed in pathological CH, however, without pathological markers. MiRNA-29c expression increased in T1 (52%) and T2 (123%), correlated with a decrease in COLIAI and COLIIIAI expression in T1 (27%, 38%) and T2 (33%, 48%), respectively. MiRNA-29c was inversely correlated to OH-proline concentration (r = 0.61, P = 0.05). The E/A ratio increased in T2, indicating improved LV compliance. Thus, these results show that aerobic training increase miR-29 expression and decreased collagen gene expression and concentration in the heart, which is relevant to the improved LV compliance and beneficial cardiac effects, associated with aerobic high performance training.
Palavras-chave
cardiac hypertrophy, collagen, molecular markers, swimming training, physiological cardiac hypertrophy, diastolic function
URI
https://observatorio.fm.usp.br/handle/OPI/23946
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Coleções
Artigos e Materiais de Revistas Científicas - HC/InCor
Artigos e Materiais de Revistas Científicas - LIM/59