Photodynamic Therapy Using Methylene Blue to Treat Cutaneous Leishmaniasis

Nenhuma Miniatura disponível
Citações na Scopus
62
Tipo de produção
article
Data de publicação
2011
Editora
MARY ANN LIEBERT INC
DOI
Indexadores
Scopus
Web of Science
PubMed
Título da Revista
ISSN da Revista
Título do Volume
Autores
SONG, Dennis
OYAFUSO, Luiza Keiko
CARDOSO, Joao Luiz
UCHOA, Adjaci F.
TARDIVO, Joao Paulo
BAPTISTA, Mauricio S.
Autor de Grupo de pesquisa
Editores
Coordenadores
Organizadores
Citação
PHOTOMEDICINE AND LASER SURGERY, v.29, n.10, p.711-715, 2011
Projetos de Pesquisa
Unidades Organizacionais
Fascículo
Resumo
Objective: The purpose of this study was to show the efficiency and underlying mechanism of action of photodynamic therapy (PDT) using methylene blue (MB) and non-coherent light sources to treat cutaneous leishmaniasis (CL). Background data: Systemic treatment can cause severe side effects, and PDT using porphyrin precursors as sensitizers has been used as an alternative to treat CL. MB has been used under illumination or in the dark to treat a wide range of medical conditions, and it exhibits antimicrobial activity against protozoa and viruses. Methods: In in vitro tests, the cell viability (via a MTT colorimetric assay) of Leishmania amazonensis parasites was evaluated as a function of MB concentration. In in vivo experiments, we analyzed the treatment of two lesions from a patient with leishmaniasis. The patient received a low dose of pentavalent antimony (SbV), and one lesion was treated with PDT. Results: We observed IC(50) decreases from 100 to 20 mu M in response to PDT when MB was used in different concentrations in in vitro tests. Use of SbV in combination with the PDT protocol produced faster wound recovery when compared with the use of SbV alone. Conclusions: The in vitro experiments and the results from the clinical case suggest that the inexpensive PDT protocol that is based on MB and RL50 (R) may be used to treat CL caused by L. amazonensis.
Palavras-chave
URI
https://observatorio.fm.usp.br/handle/OPI/24113
Referências
  1. Tardivo JP, 2005, PHOTODIAGN PHOTODYN, V2, P175, DOI 10.1016/S1572-1000(05)00097-9
  2. Sohl Sirius, 2007, J Dtsch Dermatol Ges, V5, P128, DOI 10.1111/j.1610-0387.2007.06177.x
  3. Akilov OE, 2007, PHOTOCH PHOTOBIO SCI, V6, P1067, DOI 10.1039/b703521g
  4. Croft SL, 2003, TRENDS PARASITOL, V19, P502, DOI 10.1016/j.pt.2003.09.008
  5. Tardivo JP, 2004, PHOTODIAGN PHOTODYN, V1, P345, DOI 10.1016/S1572-1000(05)00005-0
  6. Asilian A, 2006, CLIN EXP DERMATOL, V31, P634, DOI 10.1111/j.1365-2230.2006.02182.x
  7. Ghaffarifar F., 2006, Eastern Mediterranean Health Journal, V12, P902
  8. Junqueira HC, 2002, PHYS CHEM CHEM PHYS, V4, P2320, DOI 10.1039/b109753a
  9. Kosaka S, 2007, J INVEST DERMATOL, V127, P1546, DOI 10.1038/sj.jid.5700719
  10. Akilov OE, 2007, EXP DERMATOL, V16, P651, DOI 10.1111/j.1600-0625.2007.00578.x
  11. JONES TC, 1987, J INFECT DIS, V156, P73
  12. Akilov OE, 2006, CHEM BIOL, V13, P839, DOI 10.1016/j.chembiol.2006.06.008
  13. Oliveira-Neto MP, 1997, AM J TROP MED HYG, V57, P651
  14. Gardlo K, 2003, J AM ACAD DERMATOL, V48, P893, DOI 10.1067/mjd.2003.218
  15. Murray HW, 2005, LANCET, V366, P1561, DOI 10.1016/S0140-6736(05)67629-5
  16. Reithinger R, 2007, LANCET INFECT DIS, V7, P581, DOI 10.1016/S1473-3099(07)70209-8
  17. Bristowa CA, 2006, PHOTODIAGN PHOTODYN, V3, P162, DOI 10.1016/j.pdpdt.2006.04.004
  18. Costa JM, 2009, GM BAHIA S3, V79, P70
  19. Enk CD, 2003, HAUTARZT, V54, P506, DOI 10.1007/s00105-003-0530-5
  20. Gardlo K, 2004, Hautarzt, V55, P381, DOI 10.1007/s00105-004-0710-y
  21. GUTMANN P, 1981, KLIN WOCHENSCHR, V39, P953
  22. KIDERLEN AF, 1990, J IMMUNOL METHODS, V127, P11, DOI 10.1016/0022-1759(90)90334-R
  23. Mahajan VK, 2007, J DERMATOL TREAT, V18, P97, DOI 10.1080/09546630601159474
  24. Modabber Farrokh, 2007, Kinetoplastid Biol Dis, V6, P3, DOI 10.1186/1475-9292-6-3
  25. MOREIRA ME, 2004, J CELL PHYSL, V167, P305
  26. Muller-Breitkreutz K, 1998, J MED VIROL, V56, P239, DOI 10.1002/(SICI)1096-9071(199811)56:3<239::AID-JMV11>3.0.CO;2-9
  27. OliveiraNeto MP, 1997, PATHOL BIOL, V45, P496
  28. OLIVEIRANETO MP, 2006, REV SOC BRAS MED TRO, V39, P376
  29. ORTH K, 1998, PHOTOCHEM PHOTOBIOL, V75, P392
  30. Shih MH, 2011, INVEST OPHTH VIS SCI, V52, P223, DOI 10.1167/iovs.10-5593
  31. Tardivo JP, 2009, PHOTOMED LASER SURG, V27, P145, DOI 10.1089/pho.2008.2252

Coleções
Artigos e Materiais de Revistas Científicas - LIM/38
Artigos e Materiais de Revistas Científicas - LIM/48