Pretreatment with fish oil attenuates heart ischaemia consequences in rats

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art_SOUZA JUNIOR_Pretreatment_with_fish_oil_attenuates_heart_ischaemia_consequences_2017.PDF (1.42 MB)
Citações na Scopus
6
Tipo de produção
article
Data de publicação
2017
Editora
WILEY
DOI
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Scopus
Web of Science
PubMed
Título da Revista
ISSN da Revista
Título do Volume
Autores
SOUZA JUNIOR, Alcione Lescano de
MANCINI FILHO, Jorge
TORRES, Rosangela Pavan
CURI, Rui
Autor de Grupo de pesquisa
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Coordenadores
Organizadores
Citação
EXPERIMENTAL PHYSIOLOGY, v.102, n.11, p.1459-1473, 2017
Projetos de Pesquisa
Unidades Organizacionais
Fascículo
Resumo
New Findings What is the central question of this study? We investigated whether pretreatment with fish oil could prevent the major consequences of ischaemic injury to the heart. What is the main finding and its importance? Fish oil pretreatment attenuated the consequences of ischaemic injury as indicated by the small infarction area and the preservation of systolic function and coronary blood flow. These findings support the use of fish oil in order to reduce the impact of heart ischaemia. -3 Polyunsaturated fatty acid (-3 PUFA)-rich fish oil supplementation has protective effects on heart ischaemic injury. Left ventricular (LV) ischaemia was induced in rats by permanent ligation of the left descending coronary artery. Saline, fish oil or soybean oil was administered daily by gavage [3g(kg body weight)(-1)] for 20days before inducing ischaemia. Outcomes were assessed 24h after left descending coronary artery ligation. Pretreatment with fish oil decreased the -6/-3 fatty acid ratio in the LV. A reduction in infarct size and in the intensity of ventricular systolic dysfunction was found in the fish oil group compared with the saline or soybean oil groups through echocardiographic evaluation. Before infarction, LV glycogen concentrations were decreased in the fish oil group compared with the saline group. Soybean oil pretreatment led to a further increase in the LV levels of CINC-2/, IL-1 and TNF- induced by the heart infarction. In heart-infarcted rats, fish oil pretreatment decreased creatine kinase and caspase-3 activities; prevented the decrease in the coronary blood flow; increased LV contents of ATP and lactate; increased the mRNA levels of iNOS, eNOS, HIF1, GLUT1, VEGF- and p53 in the LV as measured by RT-PCR; and did not change LV pro-inflammatory cytokine concentrations compared with the control group. Fish oil protected the heart from ischaemia, as indicated by the decrease in the heart infarction area and systolic dysfunction associated with increased LV ATP concentrations and maintenance of the coronary blood flow with no change in pro-inflammatory cytokine levels.
Palavras-chave
omega-3 fatty acids, metabolism, heart ischemia
URI
https://observatorio.fm.usp.br/handle/OPI/24471
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Coleções
Artigos e Materiais de Revistas Científicas - HC/InCor
Artigos e Materiais de Revistas Científicas - LIM/59
Artigos e Materiais de Revistas Científicas - ODS/03