Molecular and Immnune Diagnosis: Further Testing for Human Strongyloidiasis
Nenhuma Miniatura disponível
Citações na Scopus
6
Tipo de produção
article
Data de publicação
2018
Editora
ADIS INT LTD
Indexadores
Título da Revista
ISSN da Revista
Título do Volume
Autores
BOSQUI, Larissa R.
GONCALVES-PIRES, Maria do Rosario F.
PAVANELLI, Wander R.
CONCHON-COSTA, Ivete
COSTA-CRUZ, Julia M.
COSTA, Idessania N.
Autor de Grupo de pesquisa
Editores
Coordenadores
Organizadores
Citação
MOLECULAR DIAGNOSIS & THERAPY, v.22, n.4, p.485-491, 2018
Resumo
Detection of Strongyloides stercoralis larvae is particularly challenging because only a small number of larvae are released into the feces, regardless of infection stage. Our objective was to apply conventional polymerase chain reaction (PCR) to the detection of S. stercoralis DNA in feces samples to evaluate its performance in samples of patients with strongyloidiasis and compare results with those of immunodiagnosis. Stool, serum, and saliva samples were collected from each individual (n = 48) at the clinic hospital of the State University of Londrina, Brazil, for parasitological, immunological, and molecular tests. Stool samples were processed via parasitological methods. Serum samples were used for immunoglobulin G (IgG) detection and saliva samples for IgA detection by ELISA. For amplification by conventional PCR, two different primers were used: species specific (101 bp) and genus specific (392 bp). The results showed that 34 (97.1%) of the 35 copro-positive individuals for S. stercoralis were positive for serum IgG and 19 (54.3%) were positive for salivary IgA. Regarding molecular analysis, both primers (species and genus specific) demonstrated positivity in 100% of the samples, which was confirmed by sequencing the positive samples. Complementary examinations of the parasitological method demonstrated excellent results in the context of the diagnosis of strongyloidiasis, especially in asymptomatic patients with irregular larval release in the feces.
Palavras-chave
Referências
- Agrawal V, 2009, T ROY SOC TROP MED H, V103, P242, DOI 10.1016/j.trstmh.2008.08.009
- Ahmad AF, 2013, PARASITOL RES, V112, P2811, DOI 10.1007/s00436-013-3450-z
- Atkins NS, 1997, T ROY SOC TROP MED H, V91, P609, DOI 10.1016/S0035-9203(97)90049-3
- Basuni M, 2011, AM J TROP MED HYG, V84, P338, DOI 10.4269/ajtmh.2011.10-0499
- Bisoffi Z, 2013, PLOS NEGLECT TROP D, V7, DOI 10.1371/journal.pntd.0002214
- Boggild A K, 2016, Can Commun Dis Rep, V42, P12
- Bosqui LR, 2015, ACTA TROP, V150, P190, DOI 10.1016/j.actatropica.2015.07.026
- Buonfrate D, 2018, PLOS NEGLECT TROP D, V12, DOI 10.1371/journal.pntd.0006229
- Buonfrate D, 2015, PLOS NEGLECT TROP D, V9, DOI 10.1371/journal.pntd.0003491
- Costa IN, 2003, ACTA PARASITOL, V48, P306
- de Paula FM, 2016, REV INST MED TROP SP, V58, DOI 10.1590/S1678-9946201658063
- de Paula FM, 2015, MEM I OSWALDO CRUZ, V110, P272, DOI 10.1590/0074-02760140371
- Dong LH, 2016, SCI REP-UK, V6, DOI 10.1038/srep24230
- Dorris M, 2002, INT J PARASITOL, V32, P1507, DOI 10.1016/S0020-7519(02)00156-X
- Faust E.C., 1939, Journal of Parasitology Urbana, V25, P241
- Hoffman W. A., 1934, Puerto Rico Journal of Public Health & Tropical Medicine, V9, P283
- Ines ED, 2013, DIAGN MICR INFEC DIS, V76, P31, DOI 10.1016/j.diagmicrobio.2013.01.016
- Janwan P, 2011, PARASITOL RES, V109, P1593, DOI 10.1007/s00436-011-2419-z
- Johnston Fay H, 2005, Aust J Rural Health, V13, P247, DOI 10.1111/j.1440-1584.2005.00710.x
- KATO K, 1954, JAPAN J PARASITOL, V3, P35
- Katz N, 1972, Rev Inst Med Trop Sao Paulo, V14, P397
- Khare R, 2014, J CLIN MICROBIOL, V52, P3667, DOI 10.1128/JCM.01637-14
- Llewellyn S, 2016, PLOS NEGLECT TROP D, V10, DOI 10.1371/journal.pntd.0004380
- Lodh N, 2016, ACTA TROP, V163, P9, DOI 10.1016/j.actatropica.2016.07.014
- Machado ER, 2003, MEM I OSWALDO CRUZ, V98, P849, DOI 10.1590/S0074-02762003000600024
- Marcos Luis A, 2011, Curr Infect Dis Rep, V13, P35, DOI 10.1007/s11908-010-0150-z
- Mendes T, 2017, J PARASITOLOGY RES, V2017
- Moghaddassani H, 2011, IRAN J PARASITOL, V6, P23
- Niforoushan MR, 2007, IRAN J PUBLIC HEALTH, V36, P16
- Nutman TB, 2017, PARASITOLOGY, V144, P263, DOI 10.1017/S0031182016000834
- Olsen A, 2009, T ROY SOC TROP MED H, V103, P967, DOI 10.1016/j.trstmh.2009.02.013
- Repetto SA, 2016, ACTA TROP, V157, P169, DOI 10.1016/j.actatropica.2016.02.004
- Goncalves ALR, 2012, DIAGN MICR INFEC DIS, V72, P79, DOI 10.1016/j.diagmicrobio.2011.09.011
- Ribeiro VS, 2010, IMMUNOL LETT, V134, P69, DOI 10.1016/j.imlet.2010.08.011
- RUGAI E., 1954, REV INST ADOLFO LUTZ, V14, P5
- SANGER F, 1977, P NATL ACAD SCI USA, V74, P5463, DOI 10.1073/pnas.74.12.5463
- Siddiqui AA, 2001, CLIN INFECT DIS, V33, P1040, DOI 10.1086/322707
- Sitta RB, 2014, PARASITOLOGY, V141, P716, DOI 10.1017/S0031182013002035
- Taniuchi M, 2011, AM J TROP MED HYG, V84, P332, DOI 10.4269/ajtmh.2011.10-0461
- Uparanukraw P, 1999, AM J TROP MED HYG, V60, P967, DOI 10.4269/ajtmh.1999.60.967
- Verweij JJ, 2014, CLIN MICROBIOL REV, V27, P371, DOI 10.1128/CMR.00122-13
- Verweij JJ, 2009, T ROY SOC TROP MED H, V103, P342, DOI 10.1016/j.trstmh.2008.12.001
- Yap P, 2012, JOVE-J VIS EXP, DOI 10.3791/3966
- Zueter AM, 2014, SINGAP MED J, V55, P367, DOI 10.11622/smedj.2014088