Development of a Novel Large Animal Model to Evaluate Human Dental Pulp Stem Cells for Articular Cartilage Treatment
Carregando...
Citações na Scopus
34
Tipo de produção
article
Data de publicação
2018
Editora
HUMANA PRESS INC
Indexadores
Título da Revista
ISSN da Revista
Título do Volume
Autores
SHIMOMURA, Kazunori
PINHEIRO, Carla Cristina Gomes
CAETANO, Heloisa Vasconcellos Amaral
NAKAMURA, Norimasa
BUENO, Daniela Franco
Autor de Grupo de pesquisa
Editores
Coordenadores
Organizadores
Citação
STEM CELL REVIEWS AND REPORTS, v.14, n.5, p.734-743, 2018
Resumo
Chondral lesion is a pathology with high prevalence, reaching as much as 63% of general population and 36% among athletes. The ability of human Dental Pulp Stem Cells (DPSCs) to differentiate into chondroblasts in vitro suggests that this stem cell type may be useful for tissue bioengineering. However, we have yet to identify a study of large animal models in which DPSCs were used to repair articular cartilage. Therefore, this study aimed to describe a novel treatment for cartilage lesion with DPSCs on a large animal model. Mesenchymal stem cells (MSC) were obtained from deciduous teeth and characterized by flow cytometry. DPSCs were cultured and added to a collagen type I/III biomaterial composite scaffold. Brazilian miniature pig (BR-1) was used. A 6-mm diameter, full-thickness chondral defect was created in each posterior medial condyle. The defects were covered with scaffold alone or scaffold + DPSCs on the contralateral side. Animals were euthanized 6 weeks post-surgery. Cartilage defects were analyzed macroscopically and histology according to modified O'Driscoll scoring system. Flow cytometry confirmed characterization of DPSCs as MSCs. Macroscopic and histological findings suggested that this time period was reasonable for evaluating cartilage repair. To our knowledge, this study provides the first description of an animal model using DPSCs to study the differentiation of hyaline articular cartilage in vivo. The animals tolerated the procedure well and did not show clinical or histological rejection of the DPSCs, reinforcing the feasibility of this descriptive miniature pig model for pre-clinical studies.
Palavras-chave
Mesenchymal stem cells, Tissue engineering, Human dental pulp stem cells, Articular cartilage, Hyaline cartilage, pre-clinical study, large animal model, miniature pig
Referências
- Agay D, 2010, EXP HEMATOL, V38, P945, DOI 10.1016/j.exphem.2010.06.008
- Ando W, 2008, TISSUE ENG PT A, V14, P2041, DOI 10.1089/ten.tea.2008.0015
- Ankrum JA, 2014, NAT BIOTECHNOL, V32, P252, DOI 10.1038/nbt.2816
- Ariffin SHZ, 2012, SCI WORLD J, DOI 10.1100/2012/827149
- Bueno DF, 2009, TISSUE ENG PT A, V15, P427, DOI 10.1089/ten.tea.2007.0417
- Costa AD, 2008, J CRANIOFAC SURG, V19, P204, DOI 10.1097/scs.0b013e31815c8a54
- Curl WW, 1997, ARTHROSCOPY, V13, P456, DOI 10.1016/S0749-8063(97)90124-9
- Fisher MB, 2015, TISSUE ENG PT A, V21, P850, DOI [10.1089/ten.tea.2014.0384, 10.1089/ten.TEA.2014.0384]
- Flanigan DC, 2010, MED SCI SPORT EXER, V42, P1795, DOI 10.1249/MSS.0b013e3181d9eea0
- Gomoll AH, 2010, KNEE SURG SPORT TR A, V18, P434, DOI 10.1007/s00167-010-1072-x
- Hilkens P, 2013, CELL TISSUE RES, V353, P65, DOI 10.1007/s00441-013-1630-x
- Ishikawa K, 2012, GENE THER, V19, P670, DOI 10.1038/gt.2012.3
- Karbanova J, 2011, CELLS TISSUES ORGANS, V193, P344, DOI 10.1159/000321160
- Kerkis I, 2012, TISSUE ENG PART B-RE, V18, P129, DOI [10.1089/ten.teb.2011.0327, 10.1089/ten.TEB.2011.0327]
- Liu YC, 2002, TISSUE ENG, V8, P709, DOI 10.1089/107632702760240616
- Moroni M, 2011, PLOS ONE, V6, DOI 10.1371/journal.pone.0025210
- Murray MM, 2010, J BONE JOINT SURG AM, V92A, P2039, DOI 10.2106/JBJS.I.01368
- ODRISCOLL SW, 1988, J BONE JOINT SURG AM, V70A, P595, DOI 10.2106/00004623-198870040-00017
- Pelatti MV, 2016, STEM CELL REV REP, V12, P448, DOI 10.1007/s12015-016-9659-3
- Perera JR, 2012, ANN ROY COLL SURG, V94, P381, DOI 10.1308/003588412X13171221592573
- Pierdomenico L, 2005, TRANSPLANTATION, V80, P836, DOI 10.1097/01.tp.0000173794.72151.88
- Ringe J, 2002, CELL TISSUE RES, V307, P321, DOI 10.1007/s00441-002-0525-z
- Shen J, 2014, J AM ACAD ORTHOP SUR, V22, P467, DOI 10.5435/JAAOS-22-07-467
- Shimomura K, 2015, CARTILAGE, V6, p13S, DOI 10.1177/1947603515571002
- Shimomura K, 2010, BIOMATERIALS, V31, P8004, DOI 10.1016/j.biomaterials.2010.07.017
- Stramandinoli-Zanicotti RT, 2014, J APPL ORAL SCI, V22, P218, DOI 10.1590/1678-775720130526
- Tang R, 2011, TRANSPL P, V43, P3955, DOI 10.1016/j.transproceed.2011.08.102
- Tuan RS, 2013, J AM ACAD ORTHOP SUR, V21, P303, DOI 10.5435/JAAOS-21-05-303
- Van den Borne MPJ, 2007, OSTEOARTHR CARTILAGE, V15, P1397, DOI 10.1016/j.joca.2007.05.005
- Zhang WB, 2006, TISSUE ENG, V12, P2813, DOI 10.1089/ten.2006.12.2813